http://lod.bco-dmo.org/id/dataset/997032
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-04-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Stress responses of cryptic corals from the Palau coral selection plasticity dispersal project
2026-04-23
publication
2026-04-23
revision
BCO-DMO Linked Data URI
2026-04-23
creation
http://lod.bco-dmo.org/id/dataset/997032
Carsten G.B. Grupstra
Boston University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grupstra, C. G. (2026) Stress responses of cryptic corals. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-04-23 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/997032 [access date]
Stress responses of cryptic corals Dataset Description: Methods and Sampling: <p>Colonies resembling the gross morphology of <em>Porites lobata</em> Dana, 1846 were tagged at six sites, at the Rock Islands of Palau, in November 2021 in a transect along the shoreline (N=15 per site, 90 colonies total). All colonies were sampled using a hammer and chisel between 1 and 6 meters (m) depth, with the majority between 3 and 4 m. All selected colonies were at least 1-5 m apart to reduce the risk of sampling clone mates while maximizing the probability that the colonies were exposed to similar conditions within a site. Targeted colonies were also relatively small in size (30-50 centimeters (cm)) to facilitate transportation to aquarium facilities for further analyses and experiments. The total area over which corals were collected was 250-500 square meters (m²) per site. Tissue samples were taken from the center of each colony, immediately fixed in ethanol, and stored at -20 degrees Celsius (°C) (2 × 2 cm samples). Samples were transported to Boston University where 2bRAD sequencing was conducted to resolve host genetic lineage.<br />
<br />
In April 2022, fragments from massive Porites colonies representing three cryptic lineages were collected from the tagged coral colonies described above and transported to the Palau International Coral Reef Center for a controlled thermal stress experiment. The 25-day thermal challenge consisted of a control treatment held at ~29.5 °C and a heat-stress treatment in which temperatures were raised ~3 °C over seven days, held for 12 days, and then increased by an additional ~1 °C to simulate an acute heatwave. Each colony contributed replicate fragments to both treatments. Maximum photochemical efficiency of photosystem II (Fv/Fm) was measured daily or semi-daily following &gt;90 minutes dark acclimation using pulse-amplitude modulated (PAM) fluorometry to assess symbiont photophysiological stress. Coral fragments were photographed with a color standard at six timepoints, and bleaching severity was quantified as changes in grayscale intensity (paling) using ImageJ. Fragment survival was monitored throughout the experiment and analyzed using Kaplan-Meier survival estimates. Lineage- and treatment-specific responses in Fv/Fm, bleaching, and mortality were evaluated using linear mixed-effects models with colony identity as a random effect to account for repeated measurements.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2048589 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2048589
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2048678 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2048678
onGoing
Carsten G.B. Grupstra
Boston University
777 Glades Road
Boca Raton
FL
33431
USA
Carsten Grupstra
pointOfContact
asNeeded
Dataset Version: 1
Unknown
pulse-amplitude modulated (PAM) fluorometer (Junior PAM (Heinz Walz GmbH))
Hammer and chisel
Onset HOBO Pendant® Temperature Data Logger (UA-001-64)
Olympus Tough TG-6 DSLR with standardized underwater color calibration card
theme
None, User defined
Fluorometer
Manual Biota Sampler
Onset HOBO Pendant MX2201 temperature logger
Underwater Camera
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: How do selection, plasticity, and dispersal interact to determine coral success in warmer and more variable environments?
https://osprey.bco-dmo.org/project/884397
Collaborative Research: How do selection, plasticity, and dispersal interact to determine coral success in warmer and more variable environments?
<p><strong>NSF Award Abstract:</strong><br />
Coral reefs host thousands of marine species, help protect coastlines from storm damage, generate tourism, and house fish used for human consumption. However, corals are vulnerable to increasing water temperatures, which can lead to coral death. One way for reefs to survive in warming oceans is for corals that are well-suited to warmer waters to repopulate reefs that have less temperature-tolerant individuals. For this strategy to succeed, however, the more temperature-tolerant corals need to be able to disperse to and survive in these different environments. This project takes advantage of reef systems in the Pacific nation of Palau that naturally experience a wide range in temperatures across short geographic distances. Using cutting-edge ecological and genomic techniques, the team of investigators is directly testing whether young corals from Palau’s warmest reefs can successfully be carried by ocean currents to Palau’s currently cooler reefs and subsequently survive and thrive in these habitats. Given the relevance of this research for the local ecology, the team is disseminating results to the Palauan government through a written report in conjunction with Palauan scientists who are interning with the team, and to the Palauan people through public presentations. As part of this work, the investigators are maintaining a blog and are organizing a music-lecture series combining dance, music, and science to promote awareness of the coral reef crisis across English and Spanish-speaking communities in the US. Results from this project are informing restoration and conservation practices of the Coral Conservation Consortium as well as other efforts worldwide.</p>
<p>A major question in evolutionary biology is how plasticity and adaptation interact to influence survival under novel environments. Understanding these processes is increasingly important as rising temperatures associated with climate change influence species globally. For marine organisms with pelagic larval phases, including reef-building corals, the post-settlement period constitutes a critical bottleneck for adaptation and plasticity, with the added complexity that the conditions experienced and time spent as larvae can incur carryover effects. This project leverages reefs in Palau that span a steep environmental gradient to study how environmental variation drives selection and plasticity and to examine if dispersal between reefs limits success across habitats due to carryover effects. The investigators are testing the overarching hypothesis that corals from warmer and more variable environments are adapted to warmer temperatures and exhibit increased plasticity, but that dispersal between reefs incurs a fitness cost. The team integrates field and molecular techniques to: 1) investigate the degree of selection occurring on warmer and more variable reefs, 2) test whether corals transplanted to more variable environments improve their thermal tolerance through developmental plasticity, and 3) examine whether delays in metamorphosis required for dispersal across reefs comes at a fitness cost due to carryover effects.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Palau coral selection plasticity dispersal
largerWorkCitation
project
eng; USA
oceans
134.34922
134.4766
7.16162
7.36722
2022-04-26
2022-05-21
Palauan coral reefs
0
BCO-DMO catalogue of parameters from Stress responses of cryptic corals from the Palau coral selection plasticity dispersal project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/997032/data/download
download
onLine
dataset
<p>Colonies resembling the gross morphology of <em>Porites lobata</em> Dana, 1846 were tagged at six sites, at the Rock Islands of Palau, in November 2021 in a transect along the shoreline (N=15 per site, 90 colonies total). All colonies were sampled using a hammer and chisel between 1 and 6 meters (m) depth, with the majority between 3 and 4 m. All selected colonies were at least 1-5 m apart to reduce the risk of sampling clone mates while maximizing the probability that the colonies were exposed to similar conditions within a site. Targeted colonies were also relatively small in size (30-50 centimeters (cm)) to facilitate transportation to aquarium facilities for further analyses and experiments. The total area over which corals were collected was 250-500 square meters (m²) per site. Tissue samples were taken from the center of each colony, immediately fixed in ethanol, and stored at -20 degrees Celsius (°C) (2 × 2 cm samples). Samples were transported to Boston University where 2bRAD sequencing was conducted to resolve host genetic lineage.<br />
<br />
In April 2022, fragments from massive Porites colonies representing three cryptic lineages were collected from the tagged coral colonies described above and transported to the Palau International Coral Reef Center for a controlled thermal stress experiment. The 25-day thermal challenge consisted of a control treatment held at ~29.5 °C and a heat-stress treatment in which temperatures were raised ~3 °C over seven days, held for 12 days, and then increased by an additional ~1 °C to simulate an acute heatwave. Each colony contributed replicate fragments to both treatments. Maximum photochemical efficiency of photosystem II (Fv/Fm) was measured daily or semi-daily following &gt;90 minutes dark acclimation using pulse-amplitude modulated (PAM) fluorometry to assess symbiont photophysiological stress. Coral fragments were photographed with a color standard at six timepoints, and bleaching severity was quantified as changes in grayscale intensity (paling) using ImageJ. Fragment survival was monitored throughout the experiment and analyzed using Kaplan-Meier survival estimates. Lineage- and treatment-specific responses in Fv/Fm, bleaching, and mortality were evaluated using linear mixed-effects models with colony identity as a random effect to account for repeated measurements.</p>
Specified by the Principal Investigator(s)
<p>Time-series data for Fv/Fm, bleaching (grayscale intensity), and fragment survival were compiled from daily or semi-daily measurements recorded during the 25-day experiment. Fv/Fm values were extracted directly from PAM fluorometer output files following dark-acclimation. Coral photographs were standardized using a color reference card and analyzed in ImageJ to quantify bleaching as changes in mean grayscale intensity over time. Mortality was recorded as a binary state at each timepoint. All data were quality-checked for entry accuracy, consistency across timepoints, and completeness; fragments lost were removed. Final datasets were organized by fragment ID, lineage, treatment, and timepoint.</p>
Specified by the Principal Investigator(s)
currently being processed
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
pulse-amplitude modulated (PAM) fluorometer (Junior PAM (Heinz Walz GmbH))
pulse-amplitude modulated (PAM) fluorometer (Junior PAM (Heinz Walz GmbH))
PI Supplied Instrument Name: pulse-amplitude modulated (PAM) fluorometer (Junior PAM (Heinz Walz GmbH)) PI Supplied Instrument Description:Measured maximum photochemical efficiency (Fv/Fm) of photosystem II after dark acclimation. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Hammer and chisel
Hammer and chisel
PI Supplied Instrument Name: Hammer and chisel PI Supplied Instrument Description:Colonies were sampled using a hammer and chisel. Instrument Name: Manual Biota Sampler Instrument Short Name:Manual Biota Sampler Instrument Description: "Manual Biota Sampler" indicates that a sample was collected in situ by a person, possibly using a hand-held collection device such as a jar, a net, or their hands. This term could also refer to a simple tool like a hammer, saw, or other hand-held tool. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/90/
Onset HOBO Pendant® Temperature Data Logger (UA-001-64)
Onset HOBO Pendant® Temperature Data Logger (UA-001-64)
PI Supplied Instrument Name: Onset HOBO Pendant® Temperature Data Logger (UA-001-64) PI Supplied Instrument Description:Recorded tank temperatures throughout the experiment to verify treatment stability and precision. Factory-calibrated; accuracy ±0.53 °C. Instrument Name: Onset HOBO Pendant MX2201 temperature logger Instrument Short Name:Onset HOBO Pendant MX2201 Instrument Description: The Onset HOBO MX2201 is an in-situ instrument for wet or underwater applications. It supports soil temperature, temperature, and water temperature. A one-channel logger that records up to approximately 96,000 measurements or internal logger events with 8K bytes memory. It has a polypropylene housing case. Uses Bluetooth to transmit data. Can be used with a solar radiation shield. Measurement range: -20 deg C to 70 deg C. Accuracy: +/- 0.50 deg C from 0 deg C to 50 deg C. Water depth rating: 30.5 m
Olympus Tough TG-6 DSLR with standardized underwater color calibration card
Olympus Tough TG-6 DSLR with standardized underwater color calibration card
PI Supplied Instrument Name: Olympus Tough TG-6 DSLR with standardized underwater color calibration card PI Supplied Instrument Description:Used to document coral fragments at six timepoints. Color standard enabled quantitative analysis of bleaching via grayscale intensity in ImageJ. Instrument Name: Underwater Camera Instrument Short Name: Instrument Description: All types of photographic equipment that may be deployed underwater including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/180/