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            <gco:CharacterString>Cite this dataset as: Meyer-Kaiser, K., Grupstra, C. G., Bennett, M., Andres, M., Davies, S. W. (2026) Results of a field transplant experiment using newly-settled Porites corals in Palau in 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-04-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.997286.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Transplant experiment Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;We conducted an experiment to determine whether post-settlement survival differs between corals settling on their natal (home) extreme reef or at a classic (away) reef. &amp;amp;nbsp;Adult massive &amp;lt;em&amp;gt;Porites&amp;lt;/em&amp;gt; corals were collected from six sites in Palau immediately prior to their spawning season in April 2023. Corals were either whole colonies or fragments of large colonies removed with a hammer and chisel (15 – 30 cm diameter). Collected corals were held in individual plastic containers (4.2 L; 17 x 13 x 19 cm) in ambient unfiltered flow-through seawater tanks at the Palau International Coral Reef Center (PICRC). At sunset, water levels in flow-through tanks were lowered to ~15 cm so that each colony was isolated in its individual container, maintaining gametes separate until fertilization. Sperm was collected directly from male colonies using a large plastic pipette during release, and eggs were collected by pipette or by scooping from the surface with a clean petri dish. &amp;lt;em&amp;gt;Porites &amp;lt;/em&amp;gt;gametes from 3 males and 2 females were used in a bulk cross in April 2023. Two different crosses were conducted, with one male differing between the two crosses, but larvae were pooled for the experiment. One hour after fertilization began, embryos underwent a series of dilutions with 5 µm filtered seawater (FSW) and gently split into multiple containers filled with 5 µm FSW to dilute any remaining sperm and prevent polyspermy. Two days post fertilization (dpf), larvae were settled on limestone tiles (15 x 15 cm) with crushed crustose coralline algae (CCA) used as a cue. Tiles were maintained in individual plastic containers in flow-through seawater tanks at PICRC. Additional CCA was added to each tile on the fourth dpf to encourage additional settlement. Immediately prior to transplantation, the number of settled corals on 7 randomly-selected tiles was counted using a dissecting microscope to estimate the mean number of individuals settled on each tile (272 ± 36, mean ± SE). Nine days post fertilization, tiles were deployed to Taoch (home) and Outer Taoch (away) (n=10 tiles per site). Tiles were randomly distributed between sites and deployed in a horizontal orientation on stainless steel all-thread driven into the reef, with ~10 cm distance between the seafloor and the tile. The most densely populated side of each tile was deployed facing downward to mimic the cryptic spaces where most corals settle. Sixteen days after deployment (25 dpf), tiles were recovered and examined under a dissecting microscope to identify and count surviving individuals. The number of corals was counted on all tiles post transplantation. Light and temperature were quantified at transplant locations at each site using Hobo Pendants (Onset, Wareham, USA).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/884401.rdf" xlink:title="OCE-2048678" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2048678 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2048678</gmx:Anchor>
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Column descriptions:
Parameter,Description,Units
Date,Date of measurement,dimensionless
Time,Time of measurement,dimensionless
AM_PM,Time of day,unitless
Temp_OT,Water temperature at site Outer Taoch,degrees Celsius
Light_OT,Light intensity at site Outer Taoch,Lux
Temp_T,Water temperature at site Taoch,degrees Celsius
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Parameter,Description,Units
Date,Date of measurement,dimensionless
Time,Time of measurement,dimensionless
AM_PM,Time of day,unitless
Temp_OT,Water temperature at site Outer Taoch,degrees Celsius
Light_OT,Light intensity at site Outer Taoch,Lux
Temp_T,Water temperature at site Taoch,degrees Celsius
Light_T,Light intensity at site Taoch,Lux</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;p&amp;gt;We conducted an experiment to determine whether post-settlement survival differs between corals settling on their natal (home) extreme reef or at a classic (away) reef. &amp;amp;nbsp;Adult massive &amp;lt;em&amp;gt;Porites&amp;lt;/em&amp;gt; corals were collected from six sites in Palau immediately prior to their spawning season in April 2023. Corals were either whole colonies or fragments of large colonies removed with a hammer and chisel (15 – 30 cm diameter). Collected corals were held in individual plastic containers (4.2 L; 17 x 13 x 19 cm) in ambient unfiltered flow-through seawater tanks at the Palau International Coral Reef Center (PICRC). At sunset, water levels in flow-through tanks were lowered to ~15 cm so that each colony was isolated in its individual container, maintaining gametes separate until fertilization. Sperm was collected directly from male colonies using a large plastic pipette during release, and eggs were collected by pipette or by scooping from the surface with a clean petri dish. &amp;lt;em&amp;gt;Porites &amp;lt;/em&amp;gt;gametes from 3 males and 2 females were used in a bulk cross in April 2023. Two different crosses were conducted, with one male differing between the two crosses, but larvae were pooled for the experiment. One hour after fertilization began, embryos underwent a series of dilutions with 5 µm filtered seawater (FSW) and gently split into multiple containers filled with 5 µm FSW to dilute any remaining sperm and prevent polyspermy. Two days post fertilization (dpf), larvae were settled on limestone tiles (15 x 15 cm) with crushed crustose coralline algae (CCA) used as a cue. Tiles were maintained in individual plastic containers in flow-through seawater tanks at PICRC. Additional CCA was added to each tile on the fourth dpf to encourage additional settlement. Immediately prior to transplantation, the number of settled corals on 7 randomly-selected tiles was counted using a dissecting microscope to estimate the mean number of individuals settled on each tile (272 ± 36, mean ± SE). Nine days post fertilization, tiles were deployed to Taoch (home) and Outer Taoch (away) (n=10 tiles per site). Tiles were randomly distributed between sites and deployed in a horizontal orientation on stainless steel all-thread driven into the reef, with ~10 cm distance between the seafloor and the tile. The most densely populated side of each tile was deployed facing downward to mimic the cryptic spaces where most corals settle. Sixteen days after deployment (25 dpf), tiles were recovered and examined under a dissecting microscope to identify and count surviving individuals. The number of corals was counted on all tiles post transplantation. Light and temperature were quantified at transplant locations at each site using Hobo Pendants (Onset, Wareham, USA).&amp;lt;/p&amp;gt;</gco:CharacterString>
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            <gco:CharacterString>PI Supplied Instrument Name:  PI Supplied Instrument Description:Light and 233 temperature were quantified at transplant locations at each site using Hobo Pendants (Onset, 234 Wareham, USA). Instrument Name: Onset HOBO Pendant Temperature/Light Data Logger Instrument Short Name:Onset HOBO Pendant Temp/Light   Instrument Description: The Onset HOBO (model numbers UA-002-64 or UA-001-64) is an in-situ instrument for wet or underwater applications. It supports light intensity, soil temperature, temperature, and water temperature. A two-channel logger with 10-bit resolution can record up to approximately 28,000 combined temperature and light measurements with 64K bytes memory. It has a polypropylene housing case. Uses an optical USB to transmit data. A solar radiation shield is used for measurement in sunlight. Temperature measurement range: -20 deg C to 70 deg C (temperature). Light measurement range: 0 to 320,000 lux. Temperature accuracy: +/- 0.53 deg C from 0 deg C to 50 deg C. Light accuracy: Designed for measurement of relative light levels. Water depth rating: 30 m.</gco:CharacterString>
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