http://lod.bco-dmo.org/id/dataset/997299
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-04-22
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Nutrients and dissolved organic carbon bottle data from R/V Rachel Carson RC0078 cruise in the Salish Sea in June 2022
2026-04-22
publication
2026-04-22
revision
BCO-DMO Linked Data URI
2026-04-22
creation
http://lod.bco-dmo.org/id/dataset/997299
Anitra E. Ingalls
University of Washington
principalInvestigator
Oscar A. Sosa
University of Puget Sound
principalInvestigator
Katherine Heal
Pacific Northwest National Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sosa, O. A., Ingalls, A. E., Heal, K. (2026) Nutrients and dissolved organic carbon bottle data from R/V Rachel Carson RC0078 cruise in the Salish Sea in June 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-04-22 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/997299 [access date]
Nutrients and DOC RC0078 Dataset Description: Methods and Sampling: <p>Seawater for nutrients and dissolved organic carbon (DOC) analyses were typically collected from the CTD rosette in triplicate from different rosette bottles from two or three depths per cast.</p>
<p>For dissolved organic carbon (DOC) concentration measurements, seawater was collected directly from CTD bottles into a filtration apparatus consisting of a glass syringe connected to a filter unit loaded with 25 mm carbon-cleaned GF/F filter &nbsp;(combusted at 450 °C for 4 hours.) 2–30 mL&nbsp;of seawater was filtered into a 40 mL glass TOC “EPA” pre-chilled glass vials (about ½ to 3/4 full). Vial moisture/condensation was wiped off of the outside of the vial and immediately placed in the freezer (upright). Samples were shipped frozen to the University of Washington Marine Chemistry Laboratory for analysis.</p>
<p>Seawater samples for nutrient concentration analysis (phosphate, silicate, nitrate, nitrite, and ammonium) were collected from CTD bottles into 60 ml plastic syringes loaded with a surfactant-free cellulose membrane (25 mm, 0.45 micron pore size, Nalgene). Syringes and 60 ml HDPE sample collection bottles were rinsed twice with sample water. About 45-50 ml of seawater was filtered into the collection bottles after rinsing again with 5 mL of filtered water. Samples were frozen for storage.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2125886 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2125886
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2124712 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2124712
onGoing
Anitra E. Ingalls
University of Washington
206-221-6748
School of Oceanography Box 355351 4333 Brooklyn Ave NE
Seattle
WA
98195
USA
aingalls@u.washington.edu
pointOfContact
Oscar A. Sosa
University of Puget Sound
1500 N. Warner St.
Tacoma
WA
98416
USA
ososa@pugetsound.edu
pointOfContact
Katherine Heal
Pacific Northwest National Laboratory
School of Oceanography Box 355351 4333 Brooklyn Ave NE
Seattle
WA
98195
USA
kheal@uw.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Cruise
Station
LAT
LON
Cast
DOC
PO4
SiO4
NO3
NO2
NH4
PRS
Depth
TMP
SAL
OXY
Beam_Attenuation
Beam_Transmission
Fluorescence
PAR
pH
CTD
Seal Analytical AA3
Shimadzu TOC-Vcsh DOC analyzer
theme
None, User defined
date
Cruise identfier
station
latitude
longitude
cast
dissolved organic Carbon
reactive phosphorus (PO4)
Silicate (reactive silica)
Nitrate
Nitrite
Ammonium
water pressure
depth
water temperature at measurement depth
salinity
dissolved Oxygen
beam attenuation
transmission
fluorescence
PAR
pH
featureType
BCO-DMO Standard Parameters
CTD - profiler
Nutrient Autoanalyzer
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
RC0078
service
Deployment Activity
Seattle, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Resolving the production and fate of nitrogenous metabolites in the surface ocean
https://osprey.bco-dmo.org/project/858734
Collaborative Research: Resolving the production and fate of nitrogenous metabolites in the surface ocean
<p><em>NSF Award Abstract:</em><br />
Photosynthetic microbes provide food for nearly all other life in the ocean. Their metabolism produces organic molecules called metabolites that can leak out of cells, be intentionally excreted into seawater, or be released during cell death. Once outside the cell, these metabolites are the basis for specific interactions among microbes and determine community structure and activity. Yet, current understanding of metabolites in the ocean is limited by a historical lack of ability to measure them. The work proposed here will expand current knowledge of metabolite structures, concentrations, and production rates using recently developed analytical methods. These methods have already led to the discovery that homarine, a substituted pyridine first found in lobster in 1933, is the most abundant detectable metabolite in microbial communities of the North Pacific Ocean. While homarine is known as a predator deterrent, osmoprotectant, methyl donor, and antibiofouling agent, studies of its role in microbial community dynamics are lacking. The work proposed will clarify the role of homarine in the ocean’s microbial communities. This work will create an open-source metabolite database that will serve the broader field of metabolomics, a growing area in environmental, engineering, and medical sciences. This collaboration will also promote the careers of a graduate student and a postdoctoral researcher as well as an early career professor from an underrepresented group at a primarily undergraduate institution (PUI). Undergraduates from both institutions will contribute to project development and implementation, local cruises on the R.V. Carson, lab work, and dissemination of results. This research will be integrated into a curriculum-based research experience for undergraduates in a 200-level genetics course at the PUI, University of Puget Sound.</p>
<p>The proposed work will carry out field studies and laboratory experiments to test the hypothesis that metabolites are quantitatively significant forms of carbon and nitrogen flowing through microbial communities. The identity, quantity, and production rates of metabolites will also be determined. For homarine, the enzymes and organisms responsible for its transformations will be determined. Specific proposed activities will 1) Quantify nitrogenous metabolite pools and their net production rates (particulate and dissolved) in phytoplankton cultures and in marine surface water communities; 2) Isolate homarine consuming heterotrophic bacteria and use mutagenesis techniques, transcriptomics, and stable isotope assisted metabolomics to annotate genes and characterize the biochemical reactions involved in the degradation of homarine; 3) Carry out incubations of stable isotope labeled homarine in phytoplankton cultures, heterotrophic bacterial cultures sensitive to homarine, and natural communities to quantitatively evaluate the effect of homarine on growth, track homarine through metabolic pathways, and determine the kinetics of homarine uptake; 4) Identify homarine consumers and biochemical pathways for homarine use in the environment by mining existing environmental metatranscriptomes for homarine catabolism genes. The combination of these approaches will provide better understanding of the flow of nitrogen containing metabolites through marine microbial ecosystems. Results from this work will be disseminated through peer reviewed open-source publications as well as presentations to the scientific community and the general public.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Nitrogenous Metabolites
largerWorkCitation
project
eng; USA
oceans
Seattle, WA
-123.179
-122.46633333333
47.818166666667
48.827166666667
2022-06-03
2022-06-08
0
BCO-DMO catalogue of parameters from Nutrients and dissolved organic carbon bottle data from R/V Rachel Carson RC0078 cruise in the Salish Sea in June 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/997337.rdf
Name: Date
Units: unitless
Description: <p>Date of CTD casts (UTC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/997338.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/997339.rdf
Name: Station
Units: unitless
Description: <p>Cruise station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/997340.rdf
Name: LAT
Units: decimal degrees
Description: <p>Latitude, positive is North</p>
http://lod.bco-dmo.org/id/dataset-parameter/997342.rdf
Name: LON
Units: decimal degrees
Description: <p>Longitude, negative is West</p>
http://lod.bco-dmo.org/id/dataset-parameter/997344.rdf
Name: Cast
Units: unitless
Description: <p>Station cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/997345.rdf
Name: DOC
Units: mg C/l
Description: <p>Dissolved organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/997346.rdf
Name: PO4
Units: umol/l
Description: <p>Phosphate</p>
http://lod.bco-dmo.org/id/dataset-parameter/997347.rdf
Name: SiO4
Units: umol/l
Description: <p>Silicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/997348.rdf
Name: NO3
Units: umol/l
Description: <p>Nitrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/997349.rdf
Name: NO2
Units: umol/l
Description: <p>Nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/997350.rdf
Name: NH4
Units: umol/l
Description: <p>Ammonium</p>
http://lod.bco-dmo.org/id/dataset-parameter/997351.rdf
Name: PRS
Units: db
Description: <p>Pressure</p>
http://lod.bco-dmo.org/id/dataset-parameter/997352.rdf
Name: Depth
Units: meters
Description: <p>Depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/997354.rdf
Name: TMP
Units: degrees Celsius
Description: <p>Temperature (ITS-90)</p>
http://lod.bco-dmo.org/id/dataset-parameter/997355.rdf
Name: SAL
Units: PSU
Description: <p>Salinity, Practical</p>
http://lod.bco-dmo.org/id/dataset-parameter/997356.rdf
Name: OXY
Units: milligrams per liter (mg/l)
Description: <p>Oxygen, SBE 43</p>
http://lod.bco-dmo.org/id/dataset-parameter/997357.rdf
Name: Beam_Attenuation
Units: per meter
Description: <p>Beam attenuation</p>
http://lod.bco-dmo.org/id/dataset-parameter/997358.rdf
Name: Beam_Transmission
Units: percent
Description: <p>Beam transmission</p>
http://lod.bco-dmo.org/id/dataset-parameter/997359.rdf
Name: Fluorescence
Units: milligrams per cubic meter (mg/m^3)
Description: <p>Fluorescence, WET Labs ECO-AFL/FL</p>
http://lod.bco-dmo.org/id/dataset-parameter/997360.rdf
Name: PAR
Units: micromoles photons per square meter per second (umol photons/m^2/s^1)
Description: <p>PAR/ Irradiance, Biospherical/Licor</p>
http://lod.bco-dmo.org/id/dataset-parameter/997361.rdf
Name: pH
Units: unitless
Description: <p>pH</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/997299/data/download
download
onLine
dataset
<p>Seawater for nutrients and dissolved organic carbon (DOC) analyses were typically collected from the CTD rosette in triplicate from different rosette bottles from two or three depths per cast.</p>
<p>For dissolved organic carbon (DOC) concentration measurements, seawater was collected directly from CTD bottles into a filtration apparatus consisting of a glass syringe connected to a filter unit loaded with 25 mm carbon-cleaned GF/F filter &nbsp;(combusted at 450 °C for 4 hours.) 2–30 mL&nbsp;of seawater was filtered into a 40 mL glass TOC “EPA” pre-chilled glass vials (about ½ to 3/4 full). Vial moisture/condensation was wiped off of the outside of the vial and immediately placed in the freezer (upright). Samples were shipped frozen to the University of Washington Marine Chemistry Laboratory for analysis.</p>
<p>Seawater samples for nutrient concentration analysis (phosphate, silicate, nitrate, nitrite, and ammonium) were collected from CTD bottles into 60 ml plastic syringes loaded with a surfactant-free cellulose membrane (25 mm, 0.45 micron pore size, Nalgene). Syringes and 60 ml HDPE sample collection bottles were rinsed twice with sample water. About 45-50 ml of seawater was filtered into the collection bottles after rinsing again with 5 mL of filtered water. Samples were frozen for storage.</p>
Specified by the Principal Investigator(s)
- Loaded "RC0078_bottle_nutrients_doc.csv" as resource "rc0078_bottle_nutrients_doc" (CSV format, row 1 headers), treating "", "nd", and "NA" as missing values
- Renamed fields: "year_month_day" to "Date", "Beam.Attenuation" to "Beam_Attenuation", "Beam.Transmission" to "Beam_Transmission"
- Reordered fields to: Date, Cruise, Station, LAT, LON, Cast, DOC, PO4, SiO4, NO3, NO2, NH4, PRS, Depth, TMP, SAL, OXY, Beam_Attenuation, Beam_Transmission, Fluorescence, PAR, pH
- Output to "997299_v1_rc0078_bottle_nutrients_doc.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD
CTD
PI Supplied Instrument Name: CTD PI Supplied Instrument Description:Methods Description: Seawater for nutrients and dissolved organic carbon (DOC) analyses were typically collected from the CTD in triplicate from different rosette bottles from two or three depths per cast. Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Seal Analytical AA3
Seal Analytical AA3
PI Supplied Instrument Name: Seal Analytical AA3 PI Supplied Instrument Description:Instrument Description: Nutrients (phosphate, silicate, nitrate, nitrite, & ammonia) analyses and calibration follow the protocols of the WOCE Hydrographic Program using a Seal Analytical AA3. Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Shimadzu TOC-Vcsh DOC analyzer
Shimadzu TOC-Vcsh DOC analyzer
PI Supplied Instrument Name: Shimadzu TOC-Vcsh DOC analyzer PI Supplied Instrument Description:Instrument Description: Dissolved organic carbon aqueous concentration was measured on a Shimadzu TOC-Vcsh DOC analyzer. Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Cruise: RC0078
RC0078
R/V Rachel Carson (UW)
Community Standard Description
International Council for the Exploration of the Sea
R/V Rachel Carson (UW)
vessel
RC0078
Anitra E. Ingalls
University of Washington
R/V Rachel Carson (UW)
Community Standard Description
International Council for the Exploration of the Sea
R/V Rachel Carson (UW)
vessel