http://lod.bco-dmo.org/id/dataset/997809
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-04-30
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Depth profiles of N2O concentrations and site preference from R/V Kilo Moana KM1314 in the The North Pacific Ocean (Seattle to Honolulu, including Line P, Station ALOHA), Aug - Sept 2013
2026-04-30
publication
2026-04-30
revision
BCO-DMO Linked Data URI
2026-04-30
creation
http://lod.bco-dmo.org/id/dataset/997809
Wei Qin
University of Washington
principalInvestigator
Hui Shen
University of Washington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Shen, H., Qin, W. (2026) Depth profiles of N2O concentrations and site preference from R/V Kilo Moana KM1314 in the The North Pacific Ocean (Seattle to Honolulu, including Line P, Station ALOHA), Aug - Sept 2013. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-04-30 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/997809 [access date]
Depth profiles of N2O concentrations and site preference KM1314 Dataset Description: Methods and Sampling: <p>N<sub>2</sub>O samples were collected using Niskin bottles attached at CTD, through silicone tubing directly into Wheaton 160&nbsp;mL glass serum bottles and overflowed twice before filling. The tubing was removed, and a 1&nbsp;mL headspace was introduced into each bottle before capping with a gray butyl septum. After collection, 100&nbsp;μL of saturated mercuric chloride was added to each bottle and then resealed with the gray butyl septum and an aluminum crimp seal. Samples were stored in the dark at room temperature until analysis.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1046017 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1046017
onGoing
Wei Qin
University of Washington
601 S Goodwin Ave Room C205 CLSL
Urbana
IL
61801
USA
weiqin@illinois.edu
pointOfContact
Hui Shen
University of Washington
hui666@illinois.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Datetime_of_sampling
Latitude
Longitude
Station_ID
Depth
N2O_Concentration
d15Na
d15Nb
Sp
d15Nbulk
d17O
d18O
Thermo Fisher Scientific DeltaPLUS XP isotope ratio mass spectrometer
theme
None, User defined
ISO_DateTime_UTC
latitude
longitude
station
depth
Nitrous Oxide
d15N
stable oxygen isotope 17
stable oxygen ration 18
featureType
BCO-DMO Standard Parameters
CTD - profiler
Isotope-ratio Mass Spectrometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
KM1314
service
Deployment Activity
The North Pacific Ocean (Cruise from Seattle to Honolulu, including Line P, Station ALOHA)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dimensions of Biodiversity
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446
Dimensions of Biodiversity
(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program. [MORE from NSF]
The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.
Dimensions of Biodiversity
largerWorkCitation
program
Significance of nitrification in shaping planktonic biodiversity in the ocean
https://osprey.bco-dmo.org/project/2228
Significance of nitrification in shaping planktonic biodiversity in the ocean
<p>Microorganisms sustain the biogeochemical cycling of nitrogen, one of the most important nutrient cycles on earth. A key step in this cycle, the oxidation of ammonia to nitrite by autotrophic microorganisms, was for a century thought mediated by a few restricted bacterial genera. Significant ammonia oxidation, perhaps most, is now attributed to a previously enigmatic group of Archaea - the ammonia-oxidizing archaea (AOA) - of high abundance in both marine and terrestrial environments. The investigators prior physiological and environmental analyses, the foundation for this proposal, have shown that AOA are active within the marine photic zone and that their competitive fitness in the marine environment is at least in part attributable to an extremely high affinity for ammonia, growing at near maximum growth rates at concentrations of ammonia that would not sustain known bacterial ammonia oxidizers, and an unusual copper-based respiratory system that may render them more competitive in iron limited environments. The compelling inference from these prior analyses is that AOA alter and possibly control the forms of fixed nitrogen available to other microbial assemblages within the photic zone by converting ammonia, a nearly universally available form of nitrogen, into nitrite, a form only available to nitrite oxidizing bacteria and some phytoplankton. If correct, this has a significant impact on biodiversity.</p>
<p>The PIs will use the most recent technological advances in protein and high throughput sequencing to evaluate the significance of nitrification in shaping biodiversity (genomic and metagenomics), activity (transcriptome, proteome and stable isotope probing), and in controlling availability of an important trace element (copper). In turn, by resolving the environmental and biotic variables that influence the diversity, distribution and activity of AOA, they will advance general understanding of their taxonomy. More directly, functional knowledge of the contribution of AOA to regenerated nitrate will improve estimates of new ocean production ("biological pump") based on nitrate assimilation, which in the past has mostly neglected the importance of nitrification as a major source of nitrate. Together these studies will transform understanding of the marine nitrogen cycle, estimates of new production, and will ultimately provide a better understanding of the impact of human activity on this critical nutrient cycle.</p>
<p>The nitrogen cycle has been profoundly affected by anthropogenic inputs of reactive nitrogen into terrestrial, marine, and atmospheric systems having, or predicted to have, major impacts on marine biological production, increased N20 emissions, nitrogen pollution, and eutrophication. Likewise, there is a poor understanding of the relationship between nitrogen cycling and productivity in marine ecosystems. Marine systems are increasingly affected by ocean acidification and by atmospheric inputs of reactive nitrogen. Since both changes greatly alter nitrogen available to microorganisms, the characterization of the response of these environmentally relevant AOA is of tremendous relevance to understanding the affect of acidification and anthropogenic nitrogen inputs on major ocean processes.</p>
<p>The proposed project encompasses and integrates the three dimensions (functional genetic, and taxonomic) of biodiversity. First, the project is framed by function: microbial control of one of the most important nutrient cycles on earth, the nitrogen-cycle. Second, it is motivated by recent genetic analyses that associate activities of a novel clade of Archaea (provisionally assigned to a new kingdom within the Archaea, the Thaumarchaeota) with control of ammonia oxidation in the ocean. Third, it is built upon a compelling synthesis of physiological and environmental data that lead to its central hypothesis that by altering and possibly controlling the form of nitrogen, the AOA also alter biodiversity and ecological function in one of the most productive environments on earth. It identifies a specific taxonomic imperative. The tremendous genetic diversity among the globally abundant AOA catalogued almost exclusively by gene sequencing surveys and therefore lacking formal description makes it essential to resolve membership into ecologically relevant groups or clades as a prelude to developing a formal taxonomy. The investigators have assembled a group of researchers with specific expertise in each of dimension and uniquely qualified to address the research objectives outlined in an integrative way.</p>
Nitrification and Marine Planktonic Biodiversity
largerWorkCitation
project
eng; USA
oceans
The North Pacific Ocean (Cruise from Seattle to Honolulu, including Line P, Station ALOHA)
-150
-126
30
50
2013-08-08
2013-08-29
0
BCO-DMO catalogue of parameters from Depth profiles of N2O concentrations and site preference from R/V Kilo Moana KM1314 in the The North Pacific Ocean (Seattle to Honolulu, including Line P, Station ALOHA), Aug - Sept 2013
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1000128.rdf
Name: Datetime_of_sampling
Units: unitless
Description: <p>Date and time of sampling in UTC time zone</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000130.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sampling location</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000132.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of sampling location</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000134.rdf
Name: Station_ID
Units: unitless
Description: <p>Station identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000135.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth in meters of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000136.rdf
Name: N2O_Concentration
Units: nmol per milliliter (nmol/L)
Description: <p>N2O gas concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000137.rdf
Name: d15Na
Units: permille (‰)
Description: <p>Enrichment of the central 15N atom in N₂O relative to the natural abundance of nitrogen isotopes.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000138.rdf
Name: d15Nb
Units: permille (‰)
Description: <p>Enrichment of the outer 15N atom in N₂O relative to the natural abundance of nitrogen isotopes.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000139.rdf
Name: Sp
Units: permille (‰)
Description: <p>Difference in δ15N between the central (a) and outer (b) N atoms in the linear N2O molecule (NNO).Sp (‰)=d15Na (‰) - d15 Nb(‰)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000140.rdf
Name: d15Nbulk
Units: permille (‰)
Description: <p>Enrichment of the 15N atoms in N₂O relative to the natural abundance of nitrogen isotopes.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000141.rdf
Name: d17O
Units: permille (‰)
Description: <p>Enrichment of the 17O atoms in N₂O relative to the natural abundance of oxygen isotopes.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1000142.rdf
Name: d18O
Units: permille (‰)
Description: <p>Enrichment of the 18O atoms in N₂O relative to the natural abundance of oxygen isotopes.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/997809/data/download
download
onLine
dataset
<p>N<sub>2</sub>O samples were collected using Niskin bottles attached at CTD, through silicone tubing directly into Wheaton 160&nbsp;mL glass serum bottles and overflowed twice before filling. The tubing was removed, and a 1&nbsp;mL headspace was introduced into each bottle before capping with a gray butyl septum. After collection, 100&nbsp;μL of saturated mercuric chloride was added to each bottle and then resealed with the gray butyl septum and an aluminum crimp seal. Samples were stored in the dark at room temperature until analysis.</p>
Specified by the Principal Investigator(s)
<p>Raw isotope measurements of N2O were obtained using an automated purge-and-trap inlet coupled to isotope ratio mass spectrometry and normalized to injections of calibrated N2O reference gas. Measured ion ratios (31R, 45R, 46R relative to reference gas) were first converted to ratio-of-ratios values and corrected for peak size using a calibrated 20 V-second peak area at m/z 44. Isotopomer scrambling corrections were applied to obtain corrected 15Ra and 15Rb values following published procedures. Nitrogen and oxygen isotope ratios were then reported in delta notation (δ15Na, δ15Nb, δ15Nbulk, δ17O, δ18O, and site preference) relative to atmospheric N2 and VSMOW standards.</p>
<p>Dissolved N2O concentrations were calculated from the m/z 44 peak area using a calibrated conversion factor and a fixed extracted seawater volume of 153.8 mL. Analytical precision was determined from replicate measurements.&nbsp;</p>
Specified by the Principal Investigator(s)
* converted latitude and longitude notation from decimal, minutes, seconds to decimal degrees
* Adjusted parameter names to comply with database requirements
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Thermo Fisher Scientific DeltaPLUS XP isotope ratio mass spectrometer
Thermo Fisher Scientific DeltaPLUS XP isotope ratio mass spectrometer
PI Supplied Instrument Name: Thermo Fisher Scientific DeltaPLUS XP isotope ratio mass spectrometer Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: KM1314
KM1314
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1314
Anitra E. Ingalls
University of Washington
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel