http://lod.bco-dmo.org/id/dataset/997842
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-04-30
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Particulate carbohydrate data from cruises EN667 in the Gulf of Maine in June 2021, SR2310 along the Oregon Coast in May 2023, and AE2320 in the Sargasso Sea in September 2023 (RIPPLE1, RIPPLE2, and RIPPLE3)
2026-05-08
publication
2026-05-08
revision
BCO-DMO Linked Data URI
2026-05-08
creation
http://lod.bco-dmo.org/id/dataset/997842
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lowenstein, D., Van Mooy, B. A. (2026) Particulate carbohydrate data from cruises EN667 in the Gulf of Maine in June 2021, SR2310 along the Oregon Coast in May 2023, and AE2320 in the Sargasso Sea in September 2023 (RIPPLE1, RIPPLE2, and RIPPLE3). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-05-08 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/997842 [access date]
Particulate carbohydrates from RIPPLE cruises Dataset Description: Methods and Sampling: <p><strong>Methods on EN667:</strong><br />
One liter (L) samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47-millimeter (mm) 0.2-micrometer (um) Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.</p>
<p>Carbohydrate quantiifcation was performed according to a "standard addition" protocol as follows: particulate carbohydrates were filtered onto 47 mm 0.2 um pore-size Durapore filters, flash frozen at sea, and returned to lab. In the lab, filter was cut in half, one half was "spiked" with a known-concentration monosaccharide standard mix, and both halves were then analyzed separately using the following extraction and analysis procedure: sample filter halves were extracted in 1.8 milliliter (mL) 0.4 molar (M) hydrochloric acid for 20 hours at 100 degrees Celsius (C), dried down at 60 degrees C in a vacuum centrifuge, resuspended in milliQ water, and analyzed on a Thermo Dionex ICS 6000 anion exchange chromatography tandem pulsed amperometric detector. The instrumental ionization response factor was calculated separately for each sample to account for matrix effects, and was calculated using the difference in instrument response peak area between the non-"spiked" and "spiked" filter halves for each analyte peak (i.e. via "standard addition" protocol). The final concentration of the non-"spiked" filter half (i.e. half of the filtered sample volume) was then calculated using that response factor. Samples were then blank corrected using the average of three 2-L filtered seawater blanks collected from the surface of the Sargasso Sea in September 2023. Both raw and blank-corrected per-liter carbohydrate (galactose and glucose) values are reported in molarity (M).</p>
<p><strong>Methods on SR2320:</strong><br />
One liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.</p>
<p>Carbohydrate quantiifcation was performed according to a "standard addition" protocol as follows: particulate carbohydrates were filtered onto 47 mm 0.2 um pore-size Durapore filters, flash frozen at sea, and returned to lab. In the lab, filter was cut in half, one half was "spiked" with a known-concentration monosaccharide standard mix, and both halves were then analyzed separately using the following extraction and analysis procedure: sample filter halves were extracted in 1.8 mL 0.4 M hydrochloric acid for 20 hours at 100 degrees C, dried down at 60 degrees C in a vacuum centrifuge, resuspended in milliQ water, and analyzed on a Thermo Dionex ICS 6000 anion exchange chromatography tandem pulsed amperometric detector. The instrumental ionization response factor was calculated separately for each sample to account for matrix effects, and was calculated using the difference in instrument response peak area between the non-"spiked" and "spiked" filter halves for each analyte peak (i.e. via "standard addition" protocol). The final concentration of the non-"spiked" filter half (i.e. half of the filtered sample volume) was then calculated using that response factor. Samples were then blank corrected using the average of three 2-L filtered seawater blanks collected from the surface of the Sargasso Sea in September 2023. Both raw and blank-corrected per-liter particulate carbohydrate (galactose and glucose) values are reported in molarity (M).</p>
<p><strong>Methods on AE2320:</strong><br />
Two liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.</p>
<p>Carbohydrate quantiifcation was performed according to a "standard addition" protocol as follows: particulate carbohydrates were filtered onto 47 mm 0.2 um pore-size Durapore filters, flash frozen at sea, and returned to lab. In the lab, filter was cut in half, one half was "spiked" with a known-concentration monosaccharide standard mix, and both halves were then analyzed separately using the following extraction and analysis procedure: sample filter halves were extracted in 1.8 mL 0.4 M hydrochloric acid for 20 hours at 100 degrees C, dried down at 60 degrees C in a vacuum centrifuge, resuspended in milliQ water, and analyzed on a Thermo Dionex ICS 6000 anion exchange chromatography tandem pulsed amperometric detector. The instrumental ionization response factor was calculated separately for each sample to account for matrix effects, and was calculated using the difference in instrument response peak area between the non-"spiked" and "spiked" filter halves for each analyte peak (i.e. via "standard addition" protocol). The final concentration of the non-"spiked" filter half (i.e. half of the filtered sample volume) was then calculated using that response factor. Samples were then blank corrected using the average of three 2-L filtered seawater blanks collected from the surface of the Sargasso Sea in September 2023. Both raw and blank-corrected per-liter particulate carbohydrate (galactose and glucose) values are reported in molarity (M).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2022597 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2022597
onGoing
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
(508) 289 2322
Department of Marine Chemistry and Geochemistry, MS #4, Fye 117 Woods Hole Oceanographic Institution
Woods Hole
MA
02543
USA
bvanmooy@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise_ID
Cruise_Name
Lat
Long
ISO_DateTime_UTC
Cast
Depth
Gal_M
Glu_M
Gal_M_Blank_Corr
Glu_M_Blank_Corr
Thermo Dionex ICS 6000 High Performance Anion Exchange Chromatography Pulsed Amperometric Detector
theme
None, User defined
Cruise identifier
cruise name
latitude
longitude
ISO_DateTime_UTC
cast
depth
carbohydrates
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Ion Chromatograph
instrument
BCO-DMO Standard Instruments
AE2320
EN667
SR2310
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Production and Fate of Fats in the Upper Ocean
https://osprey.bco-dmo.org/project/915486
Production and Fate of Fats in the Upper Ocean
<p>The Production and Fate of Fats in the Upper Ocean Phytoplankton, microscopic photosynthetic organisms in the ocean, produce a type of fat called triaclyglycerols (TAGs). A recent discovery in the North Pacific Ocean showed that a significant percentage of primary production from phytoplankton is devoted to producing TAGs. This suggests that TAGs may serve as a source of energy for phytoplankton at night, when they are unable to generate energy from photosynthesis. Phytoplankton are the base of the food web in the ocean. Therefore, it is important to understand how they create and store energy. This project will investigate the role of fats in the ocean. Specifically, this research will look at: 1) factors that affect the production and use of TAGs by phytoplankton and 2) how TAGs contribute to the global carbon cycle. This project will support the training and education of graduate students. This project will also provide resources for mentoring high school students, support summer research experiences for high school and undergraduate students, and offer field trips for 7th grade students.</p>
<p>Triacylglycerols (TAGs) are one of the most abundant classes of lipids in the ocean. A new discovery suggests that TAGs are also a very dynamic class of biochemicals. A recent study in the surface waters of the North Pacific subtropical gyre (NPSG) showed that TAGs doubled in concentration between sunrise and sunset daily, accounting for 16 to 42% of net primary production by eukaryotic nanophytoplankton (Becker et al., 2018). These results show that TAGs are an vital component of the physiology of eukaryotic phytoplankton and that TAGs contribute significantly to the carbon cycle of the NPSG. Based on estimates from this study, daytime production of TAGs in the subtropical gyres accounted for 4 to 6 percent of total global primary production. Outside of subtropical gyres, the production rates of TAGs are entirely unknown, particularly in regions where primary production rates are higher and eukaryotic phytoplankton are more dominant. Thus, the contribution of TAGs to the global ocean carbon cycle is almost certainly underestimated. There are major outstanding questions about TAGs. What environmental factors affect rates of net TAG production? What fraction of net TAG production is exported in sinking particles? Do TAGs play a role in the food web of the euphotic zone? How much of the TAGs produced during the day do phytoplankton themselves consume at night? These questions will be answered using state-of-the-art lipidomics, in situ observations, isotope-tracing techniques, incubations, and on-deck experiments. This study will provide significant advances in our understanding of TAG metabolism in phytoplankton, elucidate the roles that TAGs play in the marine carbon cycle, constrain their global importance by studying TAGs in multiple disparate environments, and set the groundwork for future research on these fascinating and vital molecules.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
RIPPLE
largerWorkCitation
project
eng; USA
oceans
-125.276
-64.12633333
31.66283333
44.70316667
2021-06-14
2023-09-11
Gulf of Maine, Oregon coast, Sargasso Sea
0
BCO-DMO catalogue of parameters from Particulate carbohydrate data from cruises EN667 in the Gulf of Maine in June 2021, SR2310 along the Oregon Coast in May 2023, and AE2320 in the Sargasso Sea in September 2023 (RIPPLE1, RIPPLE2, and RIPPLE3)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/998273.rdf
Name: Cruise_ID
Units: unitless
Description: <p>Cruise ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/998274.rdf
Name: Cruise_Name
Units: units
Description: <p>Cruise name or nickname</p>
http://lod.bco-dmo.org/id/dataset-parameter/998275.rdf
Name: Lat
Units: decimal degrees
Description: <p>Sampling Site Latitude (North is positive)</p>
http://lod.bco-dmo.org/id/dataset-parameter/998277.rdf
Name: Long
Units: decimal degrees
Description: <p>Sampling Site Longitude (West is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/998279.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Sampling date and time (UTC) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/998281.rdf
Name: Cast
Units: unitless
Description: <p>CTD Cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/998282.rdf
Name: Depth
Units: meters
Description: <p>Depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/998283.rdf
Name: Gal_M
Units: uncorrected moles per liter
Description: <p>uncorrected particulate galactose</p>
http://lod.bco-dmo.org/id/dataset-parameter/998284.rdf
Name: Glu_M
Units: uncorrected moles per liter
Description: <p>uncorrected particulate glucose</p>
http://lod.bco-dmo.org/id/dataset-parameter/998285.rdf
Name: Gal_M_Blank_Corr
Units: blank-corrected moles per liter
Description: <p>blank-corrected particulate galactose</p>
http://lod.bco-dmo.org/id/dataset-parameter/998286.rdf
Name: Glu_M_Blank_Corr
Units: blank-corrected moles per liter
Description: <p>blank-corrected particulate glucose</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/997842/data/download
download
onLine
dataset
<p><strong>Methods on EN667:</strong><br />
One liter (L) samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47-millimeter (mm) 0.2-micrometer (um) Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.</p>
<p>Carbohydrate quantiifcation was performed according to a "standard addition" protocol as follows: particulate carbohydrates were filtered onto 47 mm 0.2 um pore-size Durapore filters, flash frozen at sea, and returned to lab. In the lab, filter was cut in half, one half was "spiked" with a known-concentration monosaccharide standard mix, and both halves were then analyzed separately using the following extraction and analysis procedure: sample filter halves were extracted in 1.8 milliliter (mL) 0.4 molar (M) hydrochloric acid for 20 hours at 100 degrees Celsius (C), dried down at 60 degrees C in a vacuum centrifuge, resuspended in milliQ water, and analyzed on a Thermo Dionex ICS 6000 anion exchange chromatography tandem pulsed amperometric detector. The instrumental ionization response factor was calculated separately for each sample to account for matrix effects, and was calculated using the difference in instrument response peak area between the non-"spiked" and "spiked" filter halves for each analyte peak (i.e. via "standard addition" protocol). The final concentration of the non-"spiked" filter half (i.e. half of the filtered sample volume) was then calculated using that response factor. Samples were then blank corrected using the average of three 2-L filtered seawater blanks collected from the surface of the Sargasso Sea in September 2023. Both raw and blank-corrected per-liter carbohydrate (galactose and glucose) values are reported in molarity (M).</p>
<p><strong>Methods on SR2320:</strong><br />
One liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.</p>
<p>Carbohydrate quantiifcation was performed according to a "standard addition" protocol as follows: particulate carbohydrates were filtered onto 47 mm 0.2 um pore-size Durapore filters, flash frozen at sea, and returned to lab. In the lab, filter was cut in half, one half was "spiked" with a known-concentration monosaccharide standard mix, and both halves were then analyzed separately using the following extraction and analysis procedure: sample filter halves were extracted in 1.8 mL 0.4 M hydrochloric acid for 20 hours at 100 degrees C, dried down at 60 degrees C in a vacuum centrifuge, resuspended in milliQ water, and analyzed on a Thermo Dionex ICS 6000 anion exchange chromatography tandem pulsed amperometric detector. The instrumental ionization response factor was calculated separately for each sample to account for matrix effects, and was calculated using the difference in instrument response peak area between the non-"spiked" and "spiked" filter halves for each analyte peak (i.e. via "standard addition" protocol). The final concentration of the non-"spiked" filter half (i.e. half of the filtered sample volume) was then calculated using that response factor. Samples were then blank corrected using the average of three 2-L filtered seawater blanks collected from the surface of the Sargasso Sea in September 2023. Both raw and blank-corrected per-liter particulate carbohydrate (galactose and glucose) values are reported in molarity (M).</p>
<p><strong>Methods on AE2320:</strong><br />
Two liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.</p>
<p>Carbohydrate quantiifcation was performed according to a "standard addition" protocol as follows: particulate carbohydrates were filtered onto 47 mm 0.2 um pore-size Durapore filters, flash frozen at sea, and returned to lab. In the lab, filter was cut in half, one half was "spiked" with a known-concentration monosaccharide standard mix, and both halves were then analyzed separately using the following extraction and analysis procedure: sample filter halves were extracted in 1.8 mL 0.4 M hydrochloric acid for 20 hours at 100 degrees C, dried down at 60 degrees C in a vacuum centrifuge, resuspended in milliQ water, and analyzed on a Thermo Dionex ICS 6000 anion exchange chromatography tandem pulsed amperometric detector. The instrumental ionization response factor was calculated separately for each sample to account for matrix effects, and was calculated using the difference in instrument response peak area between the non-"spiked" and "spiked" filter halves for each analyte peak (i.e. via "standard addition" protocol). The final concentration of the non-"spiked" filter half (i.e. half of the filtered sample volume) was then calculated using that response factor. Samples were then blank corrected using the average of three 2-L filtered seawater blanks collected from the surface of the Sargasso Sea in September 2023. Both raw and blank-corrected per-liter particulate carbohydrate (galactose and glucose) values are reported in molarity (M).</p>
Specified by the Principal Investigator(s)
- Imported the three original CSV files (SR2310_RIPPLE_2_GalGlu_data.csv, EN667_RIPPLE_1_GalGlu_data.csv, AE2320_RIPPLE_3_GalGlu_data.csv) into the BCO-DMO system.
- Treated "NA" as a missing value (missing values are empty/blank in the final CSV file).
- Concatenated the three files into a single file, adding a column for Cruise_Name.
- Converted the original "Time_UTC" column (format %m/%d/%Y %H:%M, UTC) into ISO 8601 format and renamed it to "ISO_DateTime_UTC".
- Renamed fields to comply with BCO-DMO naming conventions.
- Converted Lat from degrees-decimal_minutes to decimal degrees, applied only to rows where Cruise_ID == EN667.
- Converted Long from degrees-decimal_minutes to decimal degrees, applied only to rows where Cruise_ID == EN667.
- Rounded Lat and Long to maximum 8 decimal places (only values exceeding that precision affected).
- Saved the final file as "997842_v1_carbohydrates_ripple_cruises.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Thermo Dionex ICS 6000 High Performance Anion Exchange Chromatography Pulsed Amperometric Detector
Thermo Dionex ICS 6000 High Performance Anion Exchange Chromatography Pulsed Amperometric Detector
PI Supplied Instrument Name: Thermo Dionex ICS 6000 High Performance Anion Exchange Chromatography Pulsed Amperometric Detector PI Supplied Instrument Description:Used in analysis of carbohydrates Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)
Cruise: AE2320
AE2320
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE2320
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
Cruise: EN667
EN667
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN667
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
Cruise: SR2310
SR2310
R/V Sally Ride
Community Standard Description
International Council for the Exploration of the Sea
R/V Sally Ride
vessel
SR2310
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
R/V Sally Ride
Community Standard Description
International Council for the Exploration of the Sea
R/V Sally Ride
vessel