Dataset: DNA Extracts
Deployment: HOT_cruises

DNA Extracts
Principal Investigator: 
Edward DeLong (Massachusetts Institute of Technology, MIT-Dept CEE)
Data Manager: 
Jasmine Nahorniak (Oregon State University, OSU-CEOAS)
BCO-DMO Data Manager: 
Stephen R. Gegg (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Project: 
Current State: 
Final no updates expected
Version: 
02 June 2014
Deployment Synonyms:
 Hawaii Ocean Time-series cruises
Version Date: 
2014-06-02
Data URL:
 https://www.bco-dmo.org/dataset-deployment/517409/data

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Description

DNA extracts from the vicinity of Station ALOHA (22.75 N, 158.0 W) just north of Hawaii.

CMORE RNA and DNA Archive

Goal: The goal of this effort is to provide a time series collection of planktonic microbial DNA and RNA depth profiles from the HOT station, for CMORE research. DNA and RNA samples will be available for researchers to conduct and coordinate taxonomic and functional gene studies, for example using PCR and RT-PCR methods. Where possible, metagenomic datasets will be generated by pyrosequencing for selected profiles, and posted for downloading the blast searching on the MIT-CMORE server (http://genesis2.mit.edu/).

Activity: Collect microbial cell fraction (1.6um prefiltered, >0.22um) from HOT hydrocasts, and extract nucleic acids, for RNA and DNA for downstream analyses by CMORE investigators. Collection depths are 25m, 45m, 75m, 125m, 200m, 500m, 770m and 1000m. Extracted DNA samples will be available for distribution.

More information about this dataset deployment