Dataset: Symbiodinium genotype responses to Nitrogen
Deployment: Coffroth_2016

Symbiodinium genotype responses to nitrogen (Expt 2)

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/738228

Principal Investigator:

Casey terHorst (California State University Northridge, CSUN)

Co-Principal Investigator:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs (Host Symbiont Temp Response)

Version:

Expand/Collapse All

Description

Replicates of each Symbiodinium genotype were grown in low (25mg/L), medium (75mg/L), or high (150mg/L) nitrogen environments, after which were measured physiological parameters, including the number of cells, quantum yield, variable fluorescence, and chlorophyll content.

Methods & Sampling

Dataset acquisition description

Cultures initially isolated from Antillogorgia bipinnata coral colonies collected at Looe Key ( N 24° 32.973' W 81° 22.849; cultures 08-0689.4, 08-0689.6, 08-0691.1) in 2008 and the middle keys new Tennessee Reef (N 24° 45.150' W 81° 45.275'; cultures 13-121, 13-126) in 2013. Culture isolations were performed in the Coffroth lab, University at Buffalo. Physiological measurements were measured in the terHorst lab, California State University, Northridge. Quantum yield and fluorescence yield were measured in an AquaPen-C (Photon Systems Instruments). Chlorophyll concentration was quantified in a Triology Fluorometer. Culture growth rates were quantified by visual counts under a microscope at the beginning and end of the experiment. See Bayliss et al (2019) for further details and results.

Data Processing Description

Dataset Processing Description

BCO-DMO Processing notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1559105	NSF Division of Ocean Sciences

Instruments

Fluorescence Microscope

Supplied Name: microscope

Supplied Description:

Used to count cells for growth rate calculations.

Instrument Type

Generic Name: Fluorescence Microscope

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.

Fluorometer

Supplied Name: Trilogy Laboratory Fluorometer

Supplied Description:

Used to measure chlorophyll concentrations.

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Fluorometer

Supplied Name: AquaPen-C (Photon Systems Instruments)

Supplied Description:

Used to measure quantum yield and fluorescence yield.

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Genotype	Genetic idenetifier	unitless	sample
Ntreatment	Nitrogen level	unitless	treatment
Growthrate	Population growth rate	cells/day	growth
QY	Quantum Yield	unitless ratio	Fv_Fm
Vfl	Variable Fluorescence	unitless	Fv_Fm
Chla	Total Chlorophyll per sample	Relative Fluorescence Units	chl_a
Chlapercell	Chlorophll per cell	Relative Fluorescence Units	chl_a
lncells	Log of Cell Count	Ln(#cells)	cell_concentration

Database

Contribute Data

Dataset: Symbiodinium genotype responses to Nitrogen
Deployment: Coffroth_2016

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Symbiodinium genotype responses to NitrogenDeployment: Coffroth_2016

Dataset acquisition description

Dataset Processing Description

Dataset: Symbiodinium genotype responses to Nitrogen
Deployment: Coffroth_2016