Detailed protocols, including suggestions from the scientific community, are published on the lab website at https://u.osu.edu/viruslab/protocols/ and maintained on protocols.io at https://www.protocols.io/workspaces/sullivan-lab.
Samples were collected from the Eastern Tropical North Pacific oxygen minimum zone region (ETNP OMZ) during the OMZ Microbial Biogeochemistry Expedition cruise (R/V NewHorizon,13-28 June 2013). Seawater was collected from 16 depths spanning the mixed layer, oxycline, OMZ core, and below the OMZ. Collections were made using Niskin bottles on a rosette. Samples were preserved with EM-grade glutaraldehyde (2% final concentration), flash-frozen in liquid nitrogen and stored between -72 °C and -80 °C until analysis.
Viral and bacterial concentrations were determined based on a previously described method (Noble and Fuhrman, 1998) in which thawed samples were filtered onto 0.02-μm- pore-size filters (Anodisc, Whatman, GE Healthcare Life Sciences, Piscataway, NJ, USA), stained with SYBR Gold nucleic acid stain (Invitrogen, Life Technologies, Carlsbad, CA, USA) and enumerated using an epifluorescence microscope (Axio Imager. D2, Zeiss, Jena, Germany).
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