Dataset: Coral reef CO2 bleaching recovery - sample metadata

Name: Experimental results on coral reef CO2 bleaching recovery in Florida during 2015 (Reef coral symbioses project)
Published: 2015-10-15
Keywords: biota, oceans

Cite This Dataset

Project:

New insights into the response of reef corals to climate change using the symbiont to host cell ratio as a metric of bleaching susceptibility (Reef coral symbioses)

Principal Investigator:

Andrew Baker (University of Miami Rosenstiel School of Marine and Atmospheric Science, UM-RSMAS)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2015-10-15

Version Date:

2015-10-15

Validated:

Yes

Current State:

Final no updates expected

Experimental results on coral reef CO2 bleaching recovery in Florida during 2015 (Reef coral symbioses project)

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Description

Tissue biopsies were taken from control and bleached corals of Montastraea cavernosa during ~5 months of bleaching and recovery under two pCO2 levels. This experimental study was undertaken in tanks at the University of Miami.

Sample collection metadata is reported here. However, access to the samples themselves will be restricted until analysis is completed, typically within 2 years of collection.

Methods & Sampling

Coral tissue biopsies were taken from replicate, one-inch cores of Montastraea cavernosa as small scrapings along a polyp’s calyx wall using a razor blade. The samples were immediately placed into 300uL of 1% SDS in DNA Buffer in 1.5mL Eppendorf tubes and heated to 65°C for one hour, where they are now stable at room temperature. Small aliquots of this lysate are then removed for DNA extraction on as as-needed basis.

We retain archived lysates (consisting of SDS-lysed tissue and a small piece of skeleton) that are stable long-term (>18 years) at room temperature.

Data for this project will ultimately include symbiont:host cell ratios for these samples using a variety of Symbiodinium clade-specific probes. In addition, data may also include the results of ITS2 genotyping using Denaturing Gradient Gel Electrophoresis (DGGE). Additional data, such as environmental data collected by dataloggers during experiments or field deployments, may also be contributed.

Data Processing Description

These samples are currently being extracted and analyzed.

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date
- renamed parameters to BCO-DMO standard
- reformatted dates from m/d/y to yyyy-mm-dd
- changed NA to nd

Data Files

File
CO2_meta.csv (Comma Separated Values (.csv), 14.38 KB) MD5:8dc930466ece1e584922e691dc0dea4a Primary data file for dataset ID 616009

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1358699

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Baker_lab_2015		2015-04-04	UM-RSMAS		data info

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
treatment	either control or bleached coral tissue	unitless	treatment
sample	sample identification	unitless	sample
CO2_level	pCO2 level: control of	unitless	treatment
tank	tank number	unitless	tank
start_date	start date	yyyy-mm-dd	date_start
t_1	time 1 date post-bleaching	yyyy-mm-dd	date
t_2	time 2 date post-bleaching	yyyy-mm-dd	date
t_3	time 3 date post-bleaching	yyyy-mm-dd	date
t_4	time 4 date post-bleaching	yyyy-mm-dd	date

Status message

Database

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Dataset: Coral reef CO2 bleaching recovery - sample metadata