Dataset: Sulfide production rates Desulfovibrio hydrothermalis AM13

Name: Effects of temperature on biomass and sulfide production in Desulfovibrio hydrothermals (INSPIRE_Pyrite project)
Published: 2016-02-09
Keywords: biota, oceans

Cite This Dataset

Project:

INSPIRE Track 1: Microbial Sulfur Metabolism and its Potential for Transforming the Growth of Epitaxial Solar Cell Absorbers (INSPIRE_Pyrite)

Principal Investigator:

Peter Girguis (Harvard University)

Co-Principal Investigator:

David Clarke (Harvard University)

BCO-DMO Data Manager:

Ms Dicky Allison (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

working

Version Date:

2016-02-09

Restricted:

Validated:

Current State:

Final with updates expected

Effects of temperature on biomass and sulfide production in Desulfovibrio hydrothermals (INSPIRE_Pyrite project)

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Description

Effects of temperature on biomass and sulfide production in Desulfovibrio hydrothermalis strain AM13 across a range of biologically relevant temperatures. Measured biomass production (cell concentration in stationary phase) and maximum sulfide concentration produced and maximum sulfide production rate in the exponential phase.

Figures included with the data:

Methods & Sampling

Inoculum: 200 µl stock culture (stationary phase) into 50 ml medium (expected at t0: 8.0 E+5 cells/ml).

"The time between t0 and tend is variable. We wait to reach the stationary phase. The rate is the maximum rate (exponential phase of sulfide production)." A. Picard, personal communication.

Data Processing Description

Method for cell counting: Cell cultures were fixed with 3.7% formaldehyde for cell counts. Between 100 and 1,000 microliters of fixed sample were filtered onto a 0.2 µm GTBP polycarbonate filter (Millipore), stained with DAPI (4’,6’-diamidino-2-phenylindole; Sigma), and counted via epiflourescent microscopy, using a Zeiss Axiophot scope equipped with objectives capable of 20x, 60x and 100x magnification.

Method for sulfide concentration: Spectrophotometric quantification using standard curve after Cline, 1969.

adapted to the range of concentrations in the cultures.

Data Files

File
DhydroBioProd.csv (Comma Separated Values (.csv), 1.26 KB) MD5:6025ae334770865c9c6c7b2e019b4960 Primary data file for dataset ID 638091

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1344241

Deployments

None available yet

Instruments

Fluorescence Microscope

Supplied Name: Epifluorescence Microscope - Zeiss Axiophot

Supplied Description:

Light microscope equipped for bright field (both transmitted and reflected), phase contrast, transmitted and reflected polarization and Nomarski differential interference contrast microscopy, as well as epifluorescence.

Instrument Type

Generic Name: Fluorescence Microscope

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.

Spectrophotometer

Supplied Name: spectrophotometer

Supplied Description:

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
phase	growth phase - exponential or stationary	text	brief_desc
max_sulfide_prod	maximum sulfide concentration produced	milliMoles	sulfide
max_sulfide_prod_rate	maximum sulfide production rate	milliMoles per hour	no_bcodmo_term
numb_cells_t0	number of cells at time = 0 (beginning)	cells per milliliter	count
numb_cells_tend	number of cells at time = end (of experiment)	cells per milliliter	count
std_error_prod	standard error of maximum sulfide concentration	milliMoles	std_err
std_error_rate	standard error of maximum sulfide production rate	milliMoles per hour	std_err
temp	temperature	degrees Celsius	temp

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Dataset: Sulfide production rates Desulfovibrio hydrothermalis AM13