Dataset: Turbulence settlement: fig.3 Royal Soc. Open Sci. (2015)

Name: Experimental results on turbulence exposure in sand dollars from Bodega Marine Lab in 2014 (Turbulence-spurred settlement project)
Published: 2016-03-14
Keywords: biota, oceans

Cite This Dataset

Project:

Turbulence-spurred settlement: Deciphering a newly recognized class of larval response (Turbulence-spurred settlement)

Principal Investigator:

Brian Gaylord (University of California-Davis, UC Davis-BML)

Co-Principal Investigator:

Dr Matthew Ferner (San Francisco State University, SFSU)

Dr Christopher Lowe (Stanford University - Hopkins, Stanford-HMS)

Contact:

Dr Matthew Ferner (San Francisco State University, SFSU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2016-03-14

Version Date:

2016-03-14

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Experimental results on turbulence exposure in sand dollars from Bodega Marine Lab in 2014 (Turbulence-spurred settlement project)

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Description

Figure 3. Turbulence exposure in sand dollars shortened the time to competence by approximately 20%. On each day indicated, three replicates of 20–25 larvae each were subjected to 3 min of turbulence exposure at approximately 6 W kg−1 (500 r.p.m.), and then immediately transferred them into 40 mM excess KCl in MFSW; we also transferred three replicates of 20–25 larvae directly into 40 mM excess KCl in Millipore-filtered natural seawater (i.e. with no turbulence exposure; unmanipulated controls). All larvae in this analysiswere fromthe same batch (larval batch A, fertilized 27 May 2014).

See Hodin et al (2015) for full details.

Related Reference:
Hodin J, Ferner MC, Ng G, Lowe CJ, Gaylord B. 2015. Rethinking competence in marine life cycles: ontogenetic changes in the settlement response of sand dollar larvae exposed to turbulence. Royal Society Open Science. 2: 150114. doi: 10.1098/rsos.150114.

Data Processing Description

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- reformatted date from m/d/yyyy to yyyy-mm-dd
- reduced number of digits right of decimal
- replaced blank cells with NA ('not applicable')

Data Files

File
fig3.csv (Comma Separated Values (.csv), 1.15 KB) MD5:c9df217f4679a2a8afdd5bd59b06f5f3 Primary data file for dataset ID 640389

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1356966
NSF Division of Ocean Sciences	OCE-1357077
NSF Division of Ocean Sciences	OCE-1357033

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Gaylord_Turb-Settlement		2014-06-01	lab Bodega Marine Laboratory		data info

Instruments

Taylor-Couette system

Supplied Name:

Supplied Description:

To generate turbulence intensities (quantified in terms of the energy dissipation rate, in units ofWkg−1) ranging from those found in open ocean waters to those arising on wave-battered coasts, we employed a Taylor–Couette cell [29], an apparatus composed of two vertically oriented, coaxial cylinders separated by a 3.5mm gap that contains seawater (described in greater detail in [1]). We held the stationary inner cylinder, and thus the water in the gap, at 19–21◦C by means of a circulating water stream from a temperature-controlled water bath passing through the cylinder’s interior. During operation, the outer cylinder rotated at a prescribed speed causing relative motion between the cylinders and thereby shearing the seawater between them. At rotation speeds employed for testing sand dollar larvae, the
sheared flow was turbulent [1].

[1]Gaylord B, Hodin J, Ferner MC. 2013 Turbulent shear spurs settlement in larval sea urchins. Proc. Natl Acad. Sci. USA 110, 6901–6906. (doi:10.1073/pnas.
1220680110)

Instrument Type

Generic Name: Taylor-Couette system

Generic Description:

An apparatus composed of two vertically oriented, coaxial cylinders separated by a gap that contains seawater. During operation, the outer cylinder rotates at a prescribed speed causing relative motion between the cylinders and thereby shearing the seawater between them.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
date_expt	experiment date	yyyy-mm-dd	date
batch	larval batch identification	unitless	sample
rpm	speed of rotation creating turbulent flow; unmanip means unmanipulated/no rpm	rotations per minute	no_bcodmo_term
day_develop	age of larvae post-fertilization	days	age
settled_control	proportion of settled larvae in control replicates	larvae	count
settled_500rpm	proportion of settled larvae in treatment replicates	larvae	count
mean_settled	mean proportion of settled larvae by age	larvae	count

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Database

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Dataset: Turbulence settlement: fig.3 Royal Soc. Open Sci. (2015)