Dataset: Sample key: Dcyl_Symb_2014

Name: Sample key for Symbiodinium species and abundance in Dendrogyra cylindrus from 3 regions of the Florida Reef Tract April - Dec. 2014 (EMUCoReS project)
Published: 2017-05-17
Keywords: biota, oceans

Cite This Dataset

Spatial Extent: N:25 E:-80.4 S:24.47 W:-81.75

Temporal Extent: 2014-04-01 - 2014-12-01

Project:

RAPID: A hyper-thermal anomaly in the Florida Reef Tract: An opportunity to explore the mechanisms underpinning patterns of coral bleaching and disease (EMUCoReS)

Principal Investigator:

Dr Mauricio Rodriguez-Lanetty (Florida International University, FIU)

Co-Principal Investigator:

Dr Diego Lirman (University of Miami Rosenstiel School of Marine and Atmospheric Science, UM-RSMAS)

Dr Laurie Richardson (Florida International University, FIU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2017-05-17

Restricted:

Validated:

Current State:

Preliminary and in progress

Sample key for Symbiodinium species and abundance in Dendrogyra cylindrus from 3 regions of the Florida Reef Tract April - Dec. 2014 (EMUCoReS project)

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Description

This dataset includes a key to samples of Dendrogyra cylindrus which were analyzed for 23S on an Illumina platform from three sites in the Florida Reef Tract, one each in the upper, middle and lower Keys in 2014. Tube numbers are matched to sample name, status (pre-bleach, pale, bleached, recovery), region, locations, sampling time point, total number of colonies and number of colonies sampled.

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
sample_key_Dcyl_2014.csv (Comma Separated Values (.csv), 9.60 KB) MD5:b1b185dfd26c560003c12c9619b1608f Primary data file for dataset ID 700077

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1503430
NSF Division of Ocean Sciences	OCE-1503483

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Coral_Bleaching_FRRP		2014-01-01	shoreside Florida_Coral_Reefs	Dr Mauricio Rodriguez-Lanetty (Principal Investigator)	data info

Instruments

Automated DNA Sequencer

Supplied Name: Illumina platform

Supplied Description:

Instrument Type

Generic Name: Automated DNA Sequencer

Acronym: Automated Sequencer

Generic Description:

General term for a laboratory instrument used for deciphering the order of bases in a strand of DNA. Sanger sequencers detect fluorescence from different dyes that are used to identify the A, C, G, and T extension reactions. Contemporary or Pyrosequencer methods are based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
sample	sample tube	unitless	sample
sample_ID	sample identifier ((yyyymmdd.site.colony-num)	unitless	sample
region	region of Florida Reef Tract: upper/middle/lower	unitless	region
status	bleaching status: prebleach - April 2014; bleached - Sept 2014; Recovery – Oct 2014 and Dec 2014	unitless	status
date_sampled	date sampled	unitless	date
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is postivie	decimal degrees	lon
depth	sampling depth	meters	depth
total_num_colonies	total number of Dendrogyra cylindrus colonies	colonies	count
num_colonies_sampled	number of Dendrogyra cylindrus colonies sampled	colonies	count

Status message

Database

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Dataset: Sample key: Dcyl_Symb_2014