BCO-DMO BlogDataset: larval responses to pH variability - staining
Data Citation:
Kapsenberg, L. (2021) Biological data of mussel larvae treated with fluorescent dyes and grown in two pH treatments. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-12-20 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.751258.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
DOI:10.26008/1912/bco-dmo.751258.1
Principal Investigator:
Lydia Kapsenberg (Université Pierre et Marie Curie (Paris 6), UPMC)
Contact:
Lydia Kapsenberg (Université Pierre et Marie Curie (Paris 6), UPMC)
BCO-DMO Data Manager:
Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2018-12-20
Restricted:
No
Validated:
Yes
Current State:
Final no updates expected
Biological data of mussel larvae treated with fluorescent dyes and grown in two pH treatments.
Abstract:
Mussel larvae of Mytilus galloprovincialis were grown in two pH treatments (pH 8.1 and 7.4). Larvae were collected for biological measurements of shell field development and calcification at 35 hours post-fertilization (hpf, trochophore stage). Calcein dye was added to the cultures prior to the start of calcification. Calcofluor is live dye and so was added to sampled larvae at 35 hpf for immediate imaging. Confocal microscopy was used for 3D imaging of larvae. Images were processed in ImageJ. Shell field area was determined as the area stained by calcofluor, on one valve. Calcification area was determined as the area stained by calcein, on one valve.
©2024 Biological and Chemical Oceanography Data Management Office.
