Dataset: Narragansett Bay RWU Time-series

Name: Water characteristics measured in Narragansett Bay over two years, from March 2021 to March 2023
Published: 2023-08-21
Keywords: phytoplankton, phage, Cyanobacteria, Estuary, nutrients, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.906562.1

Spatial Extent: N:41.649737 E:-71.256378 S:41.649737 W:-71.256378

Temporal Extent: 2021-03-10 - 2023-03-29

Project:

Collaborative research: Characterization of Synechococcus-cyanophage interactions across phylogenetic and temporal scales (NB_Syn_cyanophage)

Co-Principal Investigator:

Nathan A. Ahlgren (Clark University)

Marcia Marston (Roger Williams University, RWU)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-08-21

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Water characteristics measured in Narragansett Bay over two years, from March 2021 to March 2023

Abstract:

This dataset includes basic water characteristics measured in Narragansett Bay (Rhode Island, USA) over two years as part of a study of the dynamics of marine cyanobacteria (Synechococcus) and viruses infecting cyanobacteria (cyanophage) in this estuarine ecosystem. The project principal investigators include Nathan Ahlgren (Clark University) and Marcie Marston (Roger Williams University), and the latter collected the data and water with the assistance of students and/or technicians. Water was sampled and measured once per week for a span of two years in total. Water was sampled from a dock located on the campus of Roger Williams University (RWU) at an approximate latitude and longitude coordinates of 41.649737, -71.256378. Water was collected on an incoming tide in the morning and the following parameters were measured: water temperature, salinity, in situ chlorophyll a, nitrate + nitrite, phosphate, and silicate. Water samples were collected and preserved for measurement of Synechococcus abundance by flow cytometry at a later date.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2023-08-21	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.906562.1	2024-02-06

Methods & Sampling

Water was collected at the reported time, and water collection was in nearly all cases taken on the incoming tide. Water was collected from a dock in a 1 liter (L) bottle, from which water temperature, salinity, and chlorophyll a was measured. Water samples for macronutrients were also taken from the 1 L sample bottle and filtered through a 0.45-micrometer (µm) filter and collected in duplicate and stored in 60-milliliter (mL) HDPE bottles at -20 degrees Celsius before being sent for analysis. Macronutrients (silicate, orthophosphate, ammonium, nitrite, and nitrate + nitrite) were measured from duplicate samples by the Center for Coastal Studies using a nutrient autosampler system. Duplicate 1 mL water samples were preserved with 0.125% EM grade glutaldehyde and frozen and stored at -80 degrees Celsius for flow cyometry analysis at a later time. Water temperature and salinity were measured with certified instruments. In situ chlorophyll a was measured by reading fluorescence of a whole water sample on a calibrated instrument.

Know Issues or Problems:
Sampling was done weekly in nearly all cases, and in most cases sampling was done on Wednesdays. There are a few exceptions and an additional sample on 2021-07-30 was taken two days after the 'normal' weekly sampling.

Data Processing Description

Duplicate samples for macronutrient measurements were made. This dataset reports the mean value of duplicate measurements when both measurements were above the detection limit (flag=0). In rare cases where one duplicate was measured as below detection limit and the other duplicate value was above the detection limit, the latter was reported and a flag value for that measurement is provided (flag=1).

BCO-DMO Processing

- Imported original file named "RWU_cyanophage_timeseries_env_data.csv" into the BCO-DMO system.
- Renamed fields to comply with BCO-DMO naming conventions (replaced "." with underscore).
- Converted the original date/time field to ISO 8601 format (local time zone of EST/EDT).
- Created a date/time field in ISO 8601 format in UTC time zone.
- Removed the original date/time field.
- Added columns for the sampling site latitude and longitude.
- Saved the final file as "906562_v1_narragansett_bay_time-series.csv".

Data Files

File
906562_v1_narragansett_bay_time-series.csv (Comma Separated Values (.csv), 9.83 KB) MD5:f861e10be10f01ffa222b81cc107b377 Primary data file for dataset ID 906562, version 1.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-2023175

Deployments

None available yet

Instruments

Bottle

Supplied Name: bottle

Supplied Description:

Water was collected from a dock in a 1 L bottle.

Instrument Type

Generic Name: Bottle

Generic Description:

A container, typically made of glass or plastic and with a narrow neck, used for storing drinks or other liquids.

Fluorometer

Supplied Name: Turner Designs C-FLUOR Probe (Model: 2120-000)

Supplied Description:

Used to measure in situ chlorophyll a; Fluorometer model: Turner Designs C-FLUOR Probe (Model: 2120-000) coupled with a Turner Designs DataBank Datalogger.

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Nutrient Autoanalyzer

Supplied Name: nutrient autosampler

Supplied Description:

Macronutrients (silicate, orthophosphate, ammonium, nitrite, and nitrate + nitrite) were measured from duplicate samples by the Center for Coastal Studies using a nutrient autosampler system.

Instrument Type

Generic Name: Nutrient Autoanalyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/

Generic Description:

Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples.

Refractometer

Supplied Name: Portable Refractometer

Supplied Description:

Used to measure salinity; Portable Refractometer: Model RHS-10ATC.

Instrument Type

Generic Name: Refractometer

Generic Description:

A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from Snell's law and can be calculated from the composition of the material using the Gladstone-Dale relation.

In optics the refractive index (or index of refraction) n of a substance (optical medium) is a dimensionless number that describes how light, or any other radiation, propagates through that medium.

Water Temperature Sensor

Supplied Name: VEEGEE Scientific encapsulated thermometer model #80704E

Supplied Description:

Used to measure water temperature; Thermometer model: VEEGEE Scientific encapsulated thermometer model #80704E.

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
ISO_DateTime_Local	Date and approximate time of water sampling in the local time zone (EST/EDT) in ISO 8601 format Format: %Y-%m-%dT%H:%M	unitless	ISO_DateTime_Local
ISO_DateTime_UTC	Date and approximate time of water sampling in UTC in ISO 8601 format Format: %Y-%m-%dT%H:%MZ	unitless	ISO_DateTime_UTC
WaterTemp	Water temperature	degrees Celsius	temp
AirTemp	Air temperature	degrees Celsius	no_bcodmo_term
Salinity	Water salinity	practical salinity units (PSU)	sal
ChlA	Chlorophyll a	micrograms per liter (ug/L)	chl_a
NO2NO3	Water nitrate plus nitrite; BDL = below detection limit	micromolar (uM)	temp_air
PO4	Water orthophosphate; BDL = below detection limit	micromolar (uM)	PO4
SiO4	Water silicate; BDL = below detection limit	micromolar (uM)	SiO4
NH4	Water ammonium; BDL = below detection limit	micromolar (uM)	NH4
NO2	Water nitrite; BDL = below detection limit	micromolar (uM)	NO2
NH4_flag	Flag for ammonium data. 0 = mean of duplicate measurements; 1 = value of one of the duplicates, the other duplicate was BDL.	unitless	flag
Latitude	Approximate latitude where sample was collected	decimal degrees	lat
Longitude	Approximate longitude where sample was collected	decimal degrees	lon

Database

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Dataset: Narragansett Bay RWU Time-series