Dataset: Diatom cultures used to generate DNA reference library
Data Citation:
Hamsher, S., Casamatta, D., Biddanda, B. (2023) Diatom cultures used to generate DNA reference library from samples collected from sites in Alpena, Michigan and Palm Coast, Florida between July 2021 & 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-10-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.911008.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
DOI:10.26008/1912/bco-dmo.911008.1
Spatial Extent: N:45.1984 E:-83.3245 S:41.7679 W:-83.456
Temporal Extent: 2021-07-20 - 2022-07-03
Principal Investigator:
Bopaiah Biddanda (Grand Valley State University, GVSU)
Co-Principal Investigator:
Dale Casamatta (University of North Florida, UNF)
Sarah Hamsher (Grand Valley State University, GVSU)
Student:
Davis Fray (Grand Valley State University, GVSU)
Callahan McGovern (University of North Florida, UNF)
BCO-DMO Data Manager:
Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2023-10-17
Restricted:
No
Validated:
Yes
Current State:
Final no updates expected
Diatom cultures used to generate DNA reference library from samples collected from sites in Alpena, Michigan and Palm Coast, Florida between July 2021 & 2022.
Abstract:
These data are the information for each of the cultures generated from samples collected from three sites in Alpena, Michigan, one site in Monroe, Michigan, and one site in Palm Coast, Florida. Data are for cultures sequenced using Sanger sequencing and include taxonomic identification, location and water parameter information from samples used to develop the cultures, and growth medium.
Each of these cultures was developed from high-sulfur, low-oxygen environments formed by underwater sinkholes and springs that create extreme habitats populated by microbial mat communities. Our study investigated previously undescribed diatom diversity in these habitats. Sequences from these cultures contribute to tying molecular data to morphologically identified isolates, providing a bridge between these two data types that can be used to improve metabarcoding analyses.