Dataset: DDA nutrient acquisition
View Data: Data not available yet
Data Citation:
Rynearson, T. A., Inomura, K. (2024) Hemiaulus-Richelia response to additions of ammonium and nitrate with measurements of growth, nitrogen fixation, and transcriptome sequencing. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-03-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/921924 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
Spatial Extent: N:32.42 E:-63.48 S:32.42 W:-63.48
Temporal Extent: 2018-05 - 2018-05
Project:
Principal Investigator:
Tatiana A. Rynearson (University of Rhode Island, URI-GSO)
Co-Principal Investigator:
Kei Inomura (University of Rhode Island, URI)
Student:
Samantha P. Setta (University of Rhode Island, URI-GSO)
BCO-DMO Data Manager:
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2024-03-11
Restricted:
Yes
Release Date:
2024-12-31
Validated:
No
Current State:
Data not available
Hemiaulus-Richelia response to additions of ammonium and nitrate with measurements of growth, nitrogen fixation, and transcriptome sequencing
Abstract:
Diatom-diazotroph associations (DDAs) play an important role at the base of the food web by fixing both carbon dioxide and nitrogen gas into organic matter. The ability of DDAs to fix nitrogen allows the relatively large diatom host to survive under nitrogen-deplete conditions and, thus, contribute significantly to organic carbon export in oligotrophic waters. Yet, relatively little is known about this symbiosis, in part because it has historically been difficult to maintain DDAs in culture. Here, we isolated the DDA Hemiaulus-Richelia from the Sargasso Sea and developed a protocol to maintain it in laboratory culture for long time periods (years). Experiments were conducted to determine how Hemiaulus-Richelia responds to additions of ammonium and nitrate with measurements of growth, nitrogen fixation, and transcriptome sequencing.