Dataset: Nematostella ocean acidification experiment: Planula development

Name: Larvae in the planula stage 3 days post-fertilization following parental ocean acidification during lab experiments conducted in spring 2022.
Published: 2024-03-26
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.923171.1

Laboratory at the University of Pennsylvania

Temporal Extent: 2022-01-26 - 2022-05-02

Project:

Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress (Coral Resilience)

Principal Investigator:

Katie Barott (University of Pennsylvania, Penn)

Scientist:

Kristen Brown (University of Pennsylvania, Penn)

Kelsey Speer (University of Pennsylvania, Penn)

Student:

Benjamin Glass (University of Pennsylvania, Penn)

Angela Schmitt (University of Pennsylvania, Penn)

Contact:

Benjamin Glass (University of Pennsylvania, Penn)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-03-26

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Larvae in the planula stage 3 days post-fertilization following parental ocean acidification during lab experiments conducted in spring 2022.

Abstract:

Ocean acidification (OA) resulting from anthropogenic CO2 emissions is impairing the reproduction of marine organisms. While parental exposure to OA can protect offspring via carryover effects, this phenomenon is poorly understood in many marine invertebrate taxa. We examined how parental exposure to acidified (pH 7.40) versus ambient (pH 7.72) seawater influenced reproduction and offspring performance across six gametogenic cycles (13 weeks) in the estuarine sea anemone Nematostella vectensis. This dataset pertains to the performance of larvae following parental exposure to ocean acidification, specifically larval development.

Expand/Collapse All

Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2024-03-26	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.923171.1	2024-03-26

Description

Data generated as part of a Nematostella ocean acidfication experiment published in Glass et al., 2023. (see Related Publications). Related Zenodo datasets provides further analysis and plotting of the BCO-DMO dataset here. (see Related Dataset).

Methods & Sampling

Nematostella vectensis (Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).

A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).

All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals^Ⓡ reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.

Data Processing Description

Following fertilization assays, the resulting embryos were held at room temperature (∼19–21°C) to allow them to develop into swimming (planula) larvae. Water changes were performed at 24 HPF by aspirating water from each well (12 per treatment) followed by addition of newly made 12 ppt ASW. At 3 days post-fertilization (DPF), wells were examined under a dissecting microscope and the number of swimming larvae were counted along with the number of surviving non-motile larvae; unfertilized eggs and dead larvae had begun to visibly disintegrate and were clearly distinguishable from live but non-motile larvae. The percentage of larvae in the planula stage at 3 DPF was calculated by dividing the number of swimming larvae by the total number of surviving larvae.

Data Files

File
923171_v1_planuladevelopment.csv (Comma Separated Values (.csv), 1.17 KB) MD5:7c47ccc9e501c3504fe80b3b4a84e28c Primary data file for dataset ID 923171, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1923743

Deployments

None available yet

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Treatment	Experimental treatment into which anemones were placed (ambient or acidic seawater pH)	unitless	treatment
Date	Sampling week (week 1 start = 2022-01-26)	unitless	time_point
Prct_planula	Percent of larvae in planula stage	Percentage (%)	pcent_composition

Database

Contribute Data

Dataset: Nematostella ocean acidification experiment: Planula development