Dataset: Turbulence settlement: fig.7 - Royal Soc. Open Sci. (2015)

Name: Experimental results on sand dollar larvae settlement following turbulence exposure from Bodega Marine Lab in 2014 (Turbulence-spurred settlement project)
Published: 2016-03-14
Keywords: biota, oceans

Cite This Dataset

Project:

Turbulence-spurred settlement: Deciphering a newly recognized class of larval response (Turbulence-spurred settlement)

Principal Investigator:

Brian Gaylord (University of California-Davis, UC Davis-BML)

Co-Principal Investigator:

Dr Matthew Ferner (San Francisco State University, SFSU)

Dr Christopher Lowe (Stanford University - Hopkins, Stanford-HMS)

Contact:

Dr Matthew Ferner (San Francisco State University, SFSU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2016-03-14

Version Date:

2016-03-14

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Experimental results on sand dollar larvae settlement following turbulence exposure from Bodega Marine Lab in 2014 (Turbulence-spurred settlement project)

Expand/Collapse All

Description

Figure 7. Precompetent sand dollar larvae will settle following turbulence exposure in the presence of a natural inducer. (a) Twice as many larvae settled in sand extract following a 3 min exposure to intense turbulence (approx. 6 W kg−1; unpaired t-test, t4=−3.72, p=0.02). (b) Settlement in 40 mM excess KCl with this same batch of larvae also showed approximately 20% settlement following the same level of turbulence exposure, and was elevated relative to a no-turbulence, unmanipulated control treatment (Kruskal–Wallis, H2=−9.39, p<0.01), as well as an accompanying handling control (not shown). Note that we conducted this experiment on day 12 from our 25 August 2014 fertilization, a batch of larvae that was delayed relative to our other batches due, we suspect, to colder room temperatures and slightly more crowded conditions. These day 12 larvae were approximately the same stage (using our staging scheme modified from [20]) as the day 9–10 larvae from our other fertilizations (data not shown). Error bars are s.e.m.

See Hodin et al (2015) for full details.

Related Reference:
Hodin J, Ferner MC, Ng G, Lowe CJ, Gaylord B. 2015. Rethinking competence in marine life cycles: ontogenetic changes in the settlement response of sand dollar larvae exposed to turbulence. Royal Society Open Science. 2: 150114. doi: 10.1098/rsos.150114.

Data Processing Description

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- replaced blank cells in replicate column and hypens in other columns with NA ('not applicable')

Data Files

File
fig7.csv (Comma Separated Values (.csv), 703 bytes) MD5:9d291b556e603425050359d7f34400b7 Primary data file for dataset ID 640481

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1356966
NSF Division of Ocean Sciences	OCE-1357077
NSF Division of Ocean Sciences	OCE-1357033

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Gaylord_Turb-Settlement		2014-06-01	lab Bodega Marine Laboratory		data info

Instruments

Taylor-Couette system

Supplied Name:

Supplied Description:

To generate turbulence intensities (quantified in terms of the energy dissipation rate, in units of Wkg−1) ranging from those found in open ocean waters to those arising on wave-battered coasts, we employed a Taylor–Couette cell [29], an apparatus composed of two vertically oriented, coaxial cylinders separated by a 3.5mm gap that contains seawater (described in greater detail in [1]). We held the stationary inner cylinder, and thus the water in the gap, at 19–21◦C by means of a circulating water stream from a temperature-controlled water bath passing through the cylinder’s interior. During operation, the outer cylinder rotated at a prescribed speed causing relative motion between the cylinders and thereby shearing the seawater between them. At rotation speeds employed for testing sand dollar larvae, the sheared flow was turbulent [1].

[1]Gaylord B, Hodin J, Ferner MC. 2013 Turbulent shear spurs settlement in larval sea urchins. Proc. Natl Acad. Sci. USA 110, 6901–6906. (doi:10.1073/pnas.
1220680110)

Instrument Type

Generic Name: Taylor-Couette system

Generic Description:

An apparatus composed of two vertically oriented, coaxial cylinders separated by a gap that contains seawater. During operation, the outer cylinder rotates at a prescribed speed causing relative motion between the cylinders and thereby shearing the seawater between them.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
spin_time_sec	time the larvae were subjected to turbulence in the Taylor–Couette cell	seconds	duration
replicate	replicate number	unitless	replicate
cue	type of settlement cue: KCl= chemical settlement inducer (potassium chloride); NI = natural inducer (sand)	unitless	treatment
num_in_couette	number of larvae in Taylor–Couette cell	larvae	count
time_out_of_couette	time larvae were removed from Taylor–Couette cell	HHMM	time
time_in_cue	time larvae were placed in well with settlement cue	HHMM	time
target_score_time	target time for larvae to be scored	HHMM	time
time_scored	actual time larvae were scored	HHMM	time
hrs_in_cue	time the larvae were left in settlement wells with cue material	hours	duration
num_transferred	number of larvae transferred to wells	larvae	count
num_juvs	number to larvae that metamorphosed to juveniles	larvae	count
num_recovered_in_well	the number of larvae successfully retrieved from the couette device after each treatment	larvae	count
num_settled	number of larvae that settled	larvae	count
proprtn_settled	proportion of larvae that settled	unitless	no_bcodmo_term
comment	comment	unitless	comment

Status message

Database

Contribute Data

Dataset: Turbulence settlement: fig.7 - Royal Soc. Open Sci. (2015)