Dataset: Cyanomyovirus genome sequences
Deployment: lab_UCIrvine_Martiny

Cyanomyoviruses were isolated on Synechococcus sp. WH7803 or WH8101 from surface seawater samples collected from Southern California (n=19) between 2008 and 2010; Padilla Bay, Washington (n=3) in August 2010; and Narragansett Bay, Rhode Island (n=108) from 1999 to 2015 as described in Clasen et al. (2013) and Marston et al. (2013).

Plaque-purified isolates were removed from 4°C storage and regrown on Synechococcus sp. WH7803 liquid culture. Phage genomic DNA was extracted as described in Henn et al. (2010). A genomic DNA library was prepared for Illumina sequencing as described in the “Low Sample (LS) Protocol” of the Illumina TruSeq DNA sample prep kit. Samples were sequenced on an Illumina HiSeq2000 sequencer (single read, paired-end with 100 cycles) at the UCI Genomics High-throughput Facility or on a Illumina MiSeq at the Rhode Island Genomics and Sequencing Center at the University of Rhode Island.

Related references: 

Clasen, J., Hanson, C., Ibrahim, Y., Weihe, C., Marston, M., Martiny, J., 2013. Diversity and temporal dynamics of Southern California coastal marine cyanophage isolates. Aquat Microb Ecol 69, 17-31.

Henn, M.R., Sullivan, M.B., Stange-Thomann, N., Osburne, M.S., Berlin, A.M., Kelly, L., Yandava, C., Kodira, C., Zeng, Q., Weiand, M., Sparrow, T., Saif, S., Giannoukos, G., Young, S.K., Nusbaum, C., Birren, B.W., Chisholm, S.W., 2010. Analysis of high-throughput sequencing and annotation strategies for phage genomes. PLoS ONE 5, e9083.

Marston, M. F., Taylor, S., Sme, N., Parsons, R., Noyes, T. J. E., and Martiny, J. B. H. (2013). Marine cyanophages exhibit local and regional biogeography. Environ. Microbiol. 15, 452–1463. doi: 10.1111/1462-2920.12062.