Dataset: Clownfish larval growth
Deployment: PredatorSuccess_2016

All experiments were approved by the University of Hawaii’s Institutional Animal Care & Use Committee (IACUC) under protocol #1045. For determination of growth, larval Amphiprion ocellaris were euthanized using a solution of 0.06 g/ml Ethyl 3-aminobenzoate methanesulfonate salt (MS222) (Sigma-Aldrich Inc., Saint Louis, MO, USA; catalog no. A5040-25G), preserved in a solution of 5% formalin in seawater, and measured for total length and jaw size within one week of fixation.

Total length, a metric commonly used for clownfish larvae, is the greatest straight-line distance measured between the most anterior and posterior points of the body.  Measurements of fish larvae were made using a reticle calibrated with a 2.0 mm microscope micrometer at 12x for total length and 100x for mouth gape (Wild model M5A dissecting microscope).  Upper jaw lengths were measured from the anterior-most point of the pre-maxilla to the posterior edge of the maxilla. Lower jaw lengths were measured from anterior-most part of the mandible to its posterior edge.  Mouth gape was determined using Pythagorean theorem, assuming that the jaws represent two sides of a right triangle and the hypotenuse is the expected mouth gape.