Scientific sampling event log from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from Nov. 2011 (Salp_Antarctic project)

Website: https://www.bco-dmo.org/dataset/3565
Version: final
Version Date: 2011-12-28

Project
» Population ecology of Salpa thompsoni based on molecular indicators (Salp_Antarctic)
ContributorsAffiliationRole
Bucklin, AnnUniversity of Connecticut (UConn - Avery Point)Chief Scientist
Copley, NancyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager


Coverage

Spatial Extent: N:-53.1695 E:-53.55507 S:-65.08178 W:-70.907
Temporal Extent: 2011-11-02 - 2011-12-01

Dataset Description

A digital event log to record all instrument deployments during the cruise.


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Parameters

ParameterDescriptionUnits
eventunique sampling event number derived from local YYYYMMDD.HHMM dimensionless
time_locallocal time (close to EDT) as 2400 clock HHMM format dimensionless
time_utctime (UTC) using 24 hour clock HHMMSS format dimensionless
latlatitude (North is positive; South is negative) decimal degrees
lonlongitude (East is positive; West is negative) decimal degrees
instname of sampling device dimensionless
se_flags=start of activity; e=end of activity; s/e=point activity dimensionless
stationstation number dimensionless
castcast number dimensionless
depth_wdepth of water; seafloor depth from the shipboard 12 kHz Knudsen echosounder meters
commentsfree text comment dimensionless
siname of investigator responsible for the data from a sampling device dimensionless
day_localday of month, local time
month_localmonth, local time 1-12
day_utcday of month, utc time 1-31
depthdepth of cast or tow meters
month_utcmonth of year, UTC time 1-12
station_stdstandard station number
date_utcdate, UTC in yyyyMMdd format unitless

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Deployments

LMG1110

Website
Platform
ARSV Laurence M. Gould
Report
Start Date
2011-11-02
End Date
2011-12-01
Description
UNOLS STRS record: http://strs.unols.org/Public/diu_cruise_view.aspx?cruise_id=127242 The primary science objectives of the cruise are to examine genome-wide patterns of gene expression, target gene expression levels, and patterns of population genetic diversity and structure of the Antarctic salp, Salpa thompsoni in relation to biological and physical environmental parameters in the Western Antarctic Peninsula region. High-frequency acoustics data will be used to provide information about the distribution of salps, krill, and other zooplankton. Sampling from shelf and oceanic waters between 0 and 2,000 meters will take place at selected stations using a 1-meter^2 MOCNESS to characterize the planktonic assemblage, and a Reeve net to collect live material for molecular and biochemical analysis. Environmental parameters to be measured include standard hydrographic variables (temperature, salinity, and depth), as well as fluorescence and turbidity. Water samples will be collected using a CTD rosette to determine chlorophyll concentration. An additional science objective is to develop a method of using acoustics to assess the abundance and distribution of salps in the Southern Ocean. Cruise Data Report


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Project Information

Population ecology of Salpa thompsoni based on molecular indicators (Salp_Antarctic)

Coverage: Southern Ocean


The Antarctic salp, Salpa thompsoni, is an increasingly important player in the vulnerable Antarctic Peninsula pelagic ecosystem. Observations of high abundance of Salpa thompsoni during the summer in the Southern Ocean suggest that this species is capable of rapid somatic and population growth, and frequently forms dense blooms under favorable environmental conditions. The proposed research will examine genome-wide patterns of gene expression, target gene expression levels, and patterns of population genetic diversity and structure of the target salp species. Our preliminary results and data analysis have provided a promising basis for transcriptomic studies of S. thompsoni in the Southern Ocean. The proposed next steps in our genomic/transcriptomic analysis of Salpa thompsoni are: 1) completion of a reference transcriptome as a basis for genome-wide analysis of gene expression; 2) whole transcriptome shotgun sequencing (RNA-Seq) analysis to characterize gene expression in relation to individual characteristics and environmental conditions; 3) quantitative real-time PCR (qRT-PCR) characterization and validation of gene expression for 10-20 top differentially-expressed genes; and 4) detection of strand-specific allelic variation at SNP (Single Nucleotide Polymorphic) sites to analyze clonal diversity and population genetic diversity and structure. We hypothesize that: 1) deep analysis of the Salpa thompsoni transcriptome will reveal significant associations among selected set of differentially-expressed genes and critical life history stages and events (e.g., ontogenetic maturation, sexual reproduction, senescence) of the salp; and 2) the species will show variable levels of clonal diversity and significant genetic differentiation among salp populations in different regions of the Southern Ocean. Samples will be obtained from research cruises during 2011-2013 in diverse regions of the Southern Ocean; dedicated sample and data collection will be carried out during a cruise of the R/V LM GOULD (LMG11-10) to the Western Antarctic Peninsula region in November, 2011. The significance of this effort lies in new understanding of the molecular processes underlying the complex life history and population dynamics of S. thompsoni in relation to the Antarctic pelagic ecosystem and extreme and variable environmental conditions of the Southern Ocean.

Most of the data from this project are available from the Marine Geoscience Data System (MGDS), part of IEDA and is available at http://www.marine-geo.org/tools/search/Files.php?data_set_uid=18148.



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Funding

Funding SourceAward
NSF Antarctic Sciences (NSF ANT)

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