Dataset: microcosm_summary

Name: Experimental results: summary of microcosm data from Dauphin Island Cubitainer Experiment (DICE) from samples collected by R/V E.O. Wilson in the Gulf of Mexico, Alabama (En-Gen DMSP Cycling project)
Published: 2012-11-19
Keywords: oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.3857.1

Spatial Extent: N:30.05068 E:-87.99513 S:30.05068 W:-87.99513

Temporal Extent: 2006-10 - 2006-10

Project:

En-Gen: A Functional Genomics Approach to Organic Sulfur Cycling in the Ocean (En-Gen DMSP Cycling )

Principal Investigator:

Mary Ann Moran (University of Georgia, UGA)

Co-Principal Investigator:

Ronald P. Kiene (Dauphin Island Sea Lab, DISL)

William Whitman (University of Georgia, UGA)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2012-11-19

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Experimental results: summary of microcosm data from Dauphin Island Cubitainer Experiment (DICE) from samples collected by R/V E.O. Wilson in the Gulf of Mexico, Alabama (En-Gen DMSP Cycling project)

Abstract:

Summary of parameters measured in experimental and control microcosms as part of the Dauphin Island Cubitainer Experiment (DICE).

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2012-11-19	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.3857.1	2019-11-01

Description

Summary of parameters measured in experimental and control microcosms as part of the Dauphin Island Cubitainer Experiment (DICE).

Experimental design, methods, and results are further described in Howard et al. (2010) and Rinta-Kanto et al. (2011).

Methods & Sampling

See Howard et al. 2011 and Rinta-Kanto et al. 2011 for detailed methods, summarized below:

"In October 2006, seawater was collected from surface waters (<1 m deep) in the Gulf of Mexico off the coast of Dauphin Island, AL (lat: 30 03.041N; lon: 87 59.708W). Water was filtered through a 200-um mesh into six 20-liter polyethylene Cubitainers with minimal headspace.

Three microcosms were amended with 10 um sodium nitrate (NaNO3) and 0.6 um potassium phosphate (K2HPO4) to serve as the experimental microcosms. Three microcosms were left untreated to serve as the control. The Cubitainers were maintained at 27 degrees C on a 12-hour light/dark cycle for the duration of the experiment.

Chemical and activity measurements were collected from the microcosms at the beginning of the experiment (Day 0) and every day for the duration of the experiment at the same time. Chlorophyll-a samples were collected by filtration on Whatman GF/F fiters. The filters were extracted in 90% acetone for 24 hours at -20 degrees C. The extracts were quantified by fluorometry on a Turner Designs TD-700.

Samples for dissolved dimethylsulfoniopropionate (DMSPd) were collected by filtration through GF/F filters. Total dimethylsulfoniopropionate (DMSP), dissolved + particulate, was measured by acidifying whole seawater with 5 ul per ml of 50% H2SO4. Dissolved and total DMSP were quantified as dimethylsulfide (DMS) after alkaline hydrolysis. Bacterial production was measured by [3H]leucine incorporation.

Rates of DMSPd consumption were determined by multiplying the DMSPd concentrations by their respective DMSPd loss rate constant from parallel samples. DMSPd consumption rate constants were determined by measuring the loss of tracer levels of [35S]-DMSP from the dissolved (< 0.2 um) pool over time. DMS production and DMS yield (ratio of DMS production to DMSPd consumption) were measured as described in Vila-Costa et al. (2008)"

References:
Vila-Costa, M., Kiene, R.P., and Simó, R. (2008) Seasonal variability of the dynamics of dimethylated sulfur compounds in a coastal northwest mediterranean site. Limnol Oceanogr 53: 198–211.

Data Processing Description

BCO-DMO made the following changes:
- Modified parameter names to conform with BCO-DMO naming conventions.
- Replaced blanks with 'nd' to indicate 'no data'.
- Added the site coordinates provided in the publications above.

Data Files

File
microcosm_summary.csv (Comma Separated Values (.csv), 6.85 KB) MD5:fbaa2dcd9995fc247d21a55304c233e9 Primary data file for dataset ID 3857

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0724017

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
DMSP_Dauphin_Island			R/V E.O. Wilson	Mary Ann Moran (Principal Investigator) Ronald P. Kiene (Co-Principal Investigator) William Whitman (Co-Principal Investigator)	data info

Instruments

bucket

Supplied Description:

Water was collected in the field using a clean bucket.

Instrument Type

Generic Name: bucket

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0536/

Generic Description:

A bucket used to collect surface sea water samples.

Turner Designs 700 Laboratory Fluorometer

Supplied Description:

Instrument Type

Generic Name: Turner Designs 700 Laboratory Fluorometer

Acronym: TD-700

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/

Generic Description:

The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
exp_id	Name of the experiment. DICE = Dauphin Island Cubitainer Experiment.	text	exp_id
lat	Latitude of the sample collection site. North = Positive.	decimal degree	lat
lon	Longitude of the sample collection. West = Negative.	decimal degree	lon
site_desc	Description of the sample collection site.	text	site_descrip
microcosm	Identifier for the microcosm experiment. C1 and C2 were control microcosms containing Gulf of Mexico seawater. E1 and E2 were experimental microcosms containing Gulf of Mexico seawater amended with inorganic N and P.	text	sample
microcosm_type	Type of microcosm. Experimental or Control.	text	sample_descrip
exp_day	Sequential day of the experiment. Day 0 = start of the experiment.	integer	no_bcodmo_term
leu_inc_rate	Amount of leucine incorporated into bacterial protein per day.	nM/d	no_bcodmo_term
leu_inc_rate_sd	Standard deviation of leu_inc_rate.	nM/d	no_bcodmo_term
chl_a	Concentration of chlorophyll a.	ug/L	chl_a_fluor
chl_a_sd	Standard deviation of chl_a.	ug/L	no_bcodmo_term
Fv_to_Fm	Ratio of Fv to Fm; chlorophyll fluorescence.	dimensionless	no_bcodmo_term
Fv_to_Fm_sd	Standard deviation of Fv_to_Fm.	dimensionless	no_bcodmo_term
sulfate_yield	DMS consumption - sulfate = percent of radiolabeled DMS released into the medium as sulfate.	%	no_bcodmo_term
sulfate_yield_sd	Standard deviation of sulfate_yield.	%	no_bcodmo_term
DMSP_tot	Concentration of total dimethylsulfoniopropionate (DMSP). This is the sum of particulate and dissolved DMSP (DMSPp + DMSPd).	nM	no_bcodmo_term
DMSP_tot_sd	Standard deviation of DMSP_tot.	nM	no_bcodmo_term
DMSO	Dimethylsulfoxide (DMSO) concentration.	nM	no_bcodmo_term
DMSO_sd	Standard deviation of DMSO.	nM	no_bcodmo_term
DMSO_yield	A measure of dimethylsulfide (DMS) consumption. DMSO yield = percent of radiolabeled DMS released into the medium as dimethylsulfoxide (DMSO).	%	no_bcodmo_term
DMSO_yield_sd	Standard deviation of DMSO_yield.	%	no_bcodmo_term
DMSPd	Dissolved dimethylsulfoniopropionate (DMSPd) concentration.	nM	no_bcodmo_term
DMSPd_sd	Standard deviation of DMSPd.	nM	no_bcodmo_term
DMSPd_rate_const	Dissolved dimethylsulfoniopropionate (DMSPd) rate constant; the fraction of DMSPd removed per day.	1/d	no_bcodmo_term
DMSPd_rate_const_sd	Standard deviation of DMSPd_rate_const.	1/d	no_bcodmo_term
DMSPd_consump	Dissolved dimethylsulfoniopropionate (DMSPd) consumption rate; amount of DMSPd removed per day.	nM/d	no_bcodmo_term
DMSPd_consump_sd	Standard deviation of DMSPd_consump.	nM/d	no_bcodmo_term
DMSPd_assim	A measure of dissolved dimethylsulfoniopropionate (DMSPd) consumption. assimilation = percent of radiolabeled DMSPd assimilated into bacterial biomass.	%	no_bcodmo_term
DMSPd_assim_sd	Standard deviation of DMSPd_assim.	%	no_bcodmo_term
DMSPd_uptake	A measure of dissolved dimethylsulfoniopropionate (DMSPd) consumption. uptake = percent of radiolabeled DMSPd transported into the cell.	%	no_bcodmo_term
DMSPd_uptake_sd	Standard deviation of DMSPd_uptake.	%	no_bcodmo_term
DMS	Concentration of dimethylsulfide (DMS).	nM	no_bcodmo_term
DMS_sd	Standard deviation of DMS.	nM	no_bcodmo_term
DMS_rate_const	Dimethylsulfide (DMS) rate constant; fraction of DMS removed per day.	1/d	no_bcodmo_term
DMS_rate_const_sd	Standard deviation of DMS_rate_const.	1/d	no_bcodmo_term
DMS_consump	Dimethylsulfide (DMS) consumption rate; amount of DMS removed per day.	nM/d	no_bcodmo_term
DMS_consump_sd	Standard deviation of DMS_rate.	nM/d	no_bcodmo_term
DMS_assim	A measure of dimethylsulfide (DMS) consumption. assimilation = percent of radiolabeled DMS transported into the cell and assimilated into bacterial biomass.	%	no_bcodmo_term
DMS_assim_sd	Standard deviation of DMS_assim.	%	no_bcodmo_term
DMS_yield	A measure of dissolved dimethylsulfoniopropionate (DMSPd) consumption. DMS yield = percent of radiolabeled DMSP released as dimethylsulfide (DMS).	%	no_bcodmo_term
DMS_yield_sd	Standard deviation of DMS_yield.	%	no_bcodmo_term

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