Table of Contents

• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

Total extracted chlorophyll concentrations (>0.22 µm) from the Pivers Island Coastal Observatory (PICO) from 2010 to 2012.

Note: Chlorophyll was not measured at all time points, thus, some dates have no data ('nd') in the 'chl_extracted' column.

Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Methods described in Johnson et al. 2010: Chlorophyll concentrations were measured by filtering 20 mL of seawater sample onto a 0.22 micrometer (um) pore size polycarbonate filter using gentle vacuum (<100 mm Hg) and extracting in 100% MeOH at -20 degrees C in the dark for >24 h following Holm-Hansen and Riemann (1978). Fluorescence was measured using a Turner Designs 10-AU fluorometer following Welschmeyer (1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2006).

References:
Johnson, Z.I., Shyam, R., Ritchie, A.E., Lin, Y., Mioni, C., Lance, V.P. et al. 2010. The effects of iron- and light-limitation on phytoplankton communities of deep chlorophyll maxima of the Western Pacific Ocean. Journal of Marine Research 68: 1-26. doi: 10.1357/002224010793721433

Holm-Hansen, O., and Riemann, B. 1978. Chlorophyll a determination: Improvements in methodology. Oikos 30: 438-447.

Welschmeyer, N.A. 1994. Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology and Oceanography 39: 1985-1992.

Ritchie, R. 2008. Universal chlorophyll equations for estimating chlorophylls a, b, c , and d and total chlorophylls in natural assemblages of photosynthetic organisms using acetone, methanol, or ethanol solvents. Photosynthetica 46: 115-126. doi: 10.1007/s11099-008-0019-7

Note: >0.22 um referred to as "total" or simply "chlorophyll".

Quality Scores (qflag) as follows:
1 = excellent (no known issues),
2 = suspect,
3 = poor (known reason to suspect data).

BCO-DMO Processing Notes:
- Created 'replicate' column and re-arranged data so that replicates are in rows, not columns.
- Modified parameter names to conform with BCO-DMO naming conventions.
- Replaced blanks with 'nd' to indicate 'no data'.
- Separated date into month, day, and year columns.

From the project website:
Carbon dioxide is rising at ~3% per year in the atmosphere and oceans leading to increases in dissolved inorganic carbon and a reduction in pH. This trend is expected to continue for the foreseeable future and ocean pH is predicted to decrease substantially making the ocean more acidic, potentially affecting the marine ecosystem. However, coastal estuaries are highly dynamic systems that often experience dramatic changes in environmental variables over short periods of times. In this study, the investigators are measuring key variables of the marine carbon system along with other potential forcing variables and characteristics of the ecosystem that may be affected by these pH changes. The goal of this project is to determine the time-scales and magnitude of natural variability that will be superimposed on any long term trends in ocean chemistry.

This project is associated with Ocean Acidification: microbes as sentinels of adaptive responses to multiple stressors: contrasting estuarine and open ocean environments.