Dataset: chlorophyll

Name: Extracted chlorophyll concentrations from Niskin bottle samples from the PICO time-series station (34.7181 deg N, 76.6707 deg W) from 2010-2012 (PICO project)
Published: 2013-08-30
Keywords: oceans

Cite This Dataset

Spatial Extent: N:34.7181 E:-76.6707 S:34.7181 W:-76.6707

Project:

Pivers Island Coastal Observatory (PICO)

Principal Investigator:

Zackary I. Johnson (Duke University)

Co-Principal Investigator:

Dana Hunt (Duke University)

Contact:

Zackary I. Johnson (Duke University)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

30 Aug 2013

Version Date:

2013-08-30

Validated:

Yes

Current State:

Final with updates expected

Data URL:

https://www.bco-dmo.org/dataset/4030/data

Extracted chlorophyll concentrations from Niskin bottle samples from the PICO time-series station (34.7181 deg N, 76.6707 deg W) from 2010-2012 (PICO project)

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Description

Total extracted chlorophyll concentrations (>0.22 µm) from the Pivers Island Coastal Observatory (PICO) from 2010 to 2012.

Note: Chlorophyll was not measured at all time points, thus, some dates have no data ('nd') in the 'chl_extracted' column.

Acquisition Description

Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Methods described in Johnson et al. 2010: Chlorophyll concentrations were measured by filtering 20 mL of seawater sample onto a 0.22 micrometer (um) pore size polycarbonate filter using gentle vacuum (<100 mm Hg) and extracting in 100% MeOH at -20 degrees C in the dark for >24 h following Holm-Hansen and Riemann (1978). Fluorescence was measured using a Turner Designs 10-AU fluorometer following Welschmeyer (1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2006).

References:
Johnson, Z.I., Shyam, R., Ritchie, A.E., Lin, Y., Mioni, C., Lance, V.P. et al. 2010. The effects of iron- and light-limitation on phytoplankton communities of deep chlorophyll maxima of the Western Pacific Ocean. Journal of Marine Research 68: 1-26. doi: 10.1357/002224010793721433

Holm-Hansen, O., and Riemann, B. 1978. Chlorophyll a determination: Improvements in methodology. Oikos 30: 438-447.

Welschmeyer, N.A. 1994. Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology and Oceanography 39: 1985-1992.

Ritchie, R. 2008. Universal chlorophyll equations for estimating chlorophylls a, b, c , and d and total chlorophylls in natural assemblages of photosynthetic organisms using acetone, methanol, or ethanol solvents. Photosynthetica 46: 115-126. doi: 10.1007/s11099-008-0019-7

Processing Description

Note: >0.22 um referred to as "total" or simply "chlorophyll".

Quality Scores (qflag) as follows:
1 = excellent (no known issues),
2 = suspect,
3 = poor (known reason to suspect data).

BCO-DMO Processing Notes:
- Created 'replicate' column and re-arranged data so that replicates are in rows, not columns.
- Modified parameter names to conform with BCO-DMO naming conventions.
- Replaced blanks with 'nd' to indicate 'no data'.
- Separated date into month, day, and year columns.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1031064
NSF Ocean Sciences Research Initiation Grants	OCE-RIG-1322950

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
PICO_1-301	PICO	2010-06-28	Duke University Marine Lab	Zackary I. Johnson (Chief Scientist) Dana Hunt (Co-Chief Scientist) Zackary I. Johnson (Contact)	data info

Instruments

Niskin bottle

Supplied Description:

Water was sampled using a 5 Liter niskin bottle.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Turner Designs Fluorometer 10-AU

Supplied Name: Turner Designs Fluorometer -10-AU

Supplied Description:

Instrument Type

Generic Name: Turner Designs Fluorometer 10-AU

Acronym: Turner Fluorometer 10-AU

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/

Generic Description:

The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
deployment	Deployment name/id number.	text	deployno
lat	Latitude of sampling location. Positive = North.	decimal degrees	lat
lon	Longitude of sampling location. Positive = East.	decimal degrees	lon
year	Year (local time) of the sampling event.	YYYY	year
month_local	Month (local time) when the sampling event occurred.	mm (01 to 12)	month_local
PID_num	Unique, sequential "occupation" number for sampling. (The unique time/day when sampling occurred.)	dimensionless	unknown
day_local	Day of month (local time) when the sampling event occurred.	dd (01 to 31)	day_local
time_local	Time (local) when the sampling event occurred; 24-hour clock.	HHMM.mm	time_local
time_qflag	Quality score for time_local: 1 = excellent (no known issues); 2 = suspect; 3 = poor (known reason to suspect data).	dimensionless	q_flag
replicate	Replicate identifier. (All of the "A" chlorophyll samples are from the same bottle, however "A" replicates for chlorophyll are unrelated to "A" replicates in the other PICO datasets.)	text	replicate
chl_extracted	Extracted chlorophyll concentration (>0.22 um).	milligrams Chl-a per cubic meter (mg Chl-a m-3)	chl_tot
chl_qflag	Quality score for chl_extracted: 1 = excellent (no known issues); 2 = suspect; 3 = poor (known reason to suspect data).	dimensionless	qflag
depth	Depth of water sampling.	meters	depth
yrday	Consecutive day of year for a specified year, as a decimal. The fraction of the value represents the time within the day (e.g. a value of 1.5 means January 1 at 1200 hours).	dimensionless	yrday
ISO_DateTime_Local	Date-time (local) formatted to ISO 8601 standard.	YYYY-MM-DDTHH:MM:SS.ss	ISO_DateTime_Local

Database

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Dataset: chlorophyll