Data Set: 2B
5/13/2013
Mini Remin Exp
OA6 Bioavailability Exp 2013

Is there a siginificant difference in the bioavailability of organic matter produced by D. frag as a result of varied temperature and pCO2 regimes?

Does this organic matter change become less available (higher C:N) as the phytos become more nutrient stressed?

Treatments:
1. 20deg 400ppm 2 days post nutrient depletion
2. 20deg 400ppm 4 days post nutrient depletion
3. 20deg 400ppm 6 days post nutrient depletion
4. 20deg 1000ppm 2 days post nutrient depletion
5. 20deg 1000ppm 4 days post nutrient depletion
6. 20deg 1000ppm 6 days post nutrient depletion
7. 15deg 400ppm 2 days post nutrient depletion
8. 15deg 400ppm 4 days post nutrient depletion
9. 15deg 400ppm 6 days post nutrient depletion
10. 15deg 1000ppm 2 days post nutrient depletion
11. 15deg 1000ppm 4 days post nutrient depletion
12. 15deg 1000ppm 6 days post nutrient depletion

Set-up:
Sample:
These bottles were then placed in the small incubator in the Chapman lab at 12 deg (same temperature of the innoclum during time of collection)

210ml: 20% 1.22um filtered campus point SW (collected 1000 on 03/16/2013)
Total volume per 2L PC bottle (treatment) = 1.06L

The total volume was combined at 1030 and then each 2L PC bottle (with 1.5L total volume) was split between duplicate 500ml PC bottles (the remaining volume was used for T0 sampling)

These bottles were then placed in the small incubator in the Chapman lab at 12 deg (same temperature of the innoclum during time of collection)

Sampling Schedule:
Time point Time (hrs) Date Time Samples Taken From what T0 0 5/14/2013 930 DOC, FCM From 2L PC bottles T1 5/15/2013 1230 FCM all 24 T2 5/16/2013 1210 FCM all 24 T3 3.5 5/17/2013 1100 DOC, FCM all 24 T4 5/18/2013 1400 FCM all 24 T5 5/19/2013 1030 FCM all 24 T6 148 5/20/2013 1330 FCM all 24 T7 160.75 5/21/2013 945 FCM all 24 T8 191 5/22/2013 1600 DOC, FCM all 24 TF ERH?? 6/6/2013 ERH?? DOC all 24