Dataset: recruit_seawater_chemistry

Name: Experimental results of seawater chemistry for coral recruits in variable CO2 collected from the Natl Museum Mar. Bio. and Aquar., Taiwan in 2010 (MCR LTER project, Climate_Coral_Larvae project)
Published: 2014-06-13
Keywords: oceans

Cite This Dataset

Spatial Extent: N:22.0497 E:120.6985 S:22.0497 W:120.6985

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Program:

Long Term Ecological Research network (LTER)

Lead Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Student:

Aaron M. Dufault (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Danie Kinkade (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2014-06-13

Version Date:

2014-06-13

Validated:

Current State:

Preliminary and in progress

Experimental results of seawater chemistry for coral recruits in variable CO2 collected from the Natl Museum Mar. Bio. and Aquar., Taiwan in 2010 (MCR LTER project, Climate_Coral_Larvae project)

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Description

Manipulative studies have demonstrated that ocean acidification (OA) is a threat to coral reefs, yet no experiments have employed diurnal variations in pCO2 that are ecologically relevant to many shallow reefs. Two experiments were conducted to test the response of coral recruits (less than 6 days old) to diurnally oscillating pCO2; one exposing recruits for 3 days to ambient (440 uatm), high (663 uatm) and diurnally oscillating pCO2 on a natural phase (420–596 uatm), and another exposing recruits for 6 days to ambient (456 uatm), high (837 uatm) and diurnally oscillating pCO2 on either a natural or a reverse phase (448–845 uatm).

These seawater chemistry data are published in Dufault et al. (2012), Proc. R. Soc. B. doi:10.1098/rspb.2011.2545, Table 1.

Related Datasets:

recruit_growth_weight
recruit_growth_area
recruit_suvival

Methods & Sampling

Larvae were obtained from brooding colonies coral S. caliendrum collected from 5 to 7 m deep on Hobihu Reef, Nanwan Bay, in March and June of 2010, placed into individual flow-through seawater tanks. Overflow water from each tank passed through mesh-lined (110 mm) cups that captured larvae. Following collection, larvae were settled onto clean pre-weighed glass microscope coverslips. Coverslips with coral recruits (n=18: experiment I; n=36: experiment II) were assigned randomly to the pCO2 treatments.

In experiment I, treatments consisted of steady ambient pCO2, steady high pCO2 and diurnally oscillating pCO2 on a natural phase; this design was augmented in experiment II by including a diurnally oscillating pCO2 on a reverse phase.

The DIC content of seawater was manipulated by bubbling premixed gas of a known pCO2 or by bubbling unmodified air for the ambient treatment. To mix the gas for the high pCO2 treatments, a system employing a variable-timed solenoid valve was used, which controlled the flow of air and CO2 into a mixing chamber to reach the target pCO2 of 650 matm in experiment I. This target value was selected to provide a conservative estimate for the atmospheric pCO2 by 2100 following the business as usual emission scenario A1 [30]. In experiment II, pCO2 levels were increased to 800 matm to test the effects of diurnally oscillating pCO2 levels that exceeded what is found at Hobihu reef. The solenoid valve was connected to an infrared gas analyser (S151, Qubit Systems, Ontario Canada), which monitored the output gas and provided dynamic control of the duty cycle of the solenoid, thereby providing a consistent concentration of mixed gas to the treatment tanks. Refer to the electronic supplementary material for seawater chemistry and field sampling methods.

For detailed description of methods, see Dufault et al. (2012), Proc. R. Soc. B. doi:10.1098/rspb.2011.2545

Data Processing Description

For detailed description of processing see Dufault et al. (2012), Proc. R. Soc. B. doi:10.1098/rspb.2011.2545

Data Files

File
CoralRecruit_seawater_chem.csv (Comma Separated Values (.csv), 7.36 KB) MD5:11da594cc1545f8c0a1baaaae489d8ff Primary data file for dataset ID 516783

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0844785

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
lab_Edmunds_NMMBA		2010-03-18	Natl Museum Mar. Bio. and Aquar. Taiwan	Peter J. Edmunds (Lead Principal Investigator)	data info

Instruments

Gas Analyzer

Supplied Name: infrared gas analyser

Supplied Description:

An infrared gas analyser (S151, Qubit Systems, Ontario Canada) was used to provide consistent gas mixture.

Instrument Type

Generic Name: Gas Analyzer

Generic Description:

Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
omega_X	Aragonite saturation state of expeimental tank on day of measurement, where X is equal to I for experiment I and II for experiment II.	dimensionless	OM_ar
date	Date of tank water sampling.	MMDDYYYY	date
experiment	Experiment identifier: Experiment I treatments consisted of steady ambient pCO2, steady high pCO2 and diurnally oscillating pCO2 on a natural phase. Experiment II treatments consisted of a diurnally oscillating pCO2 on a reverse phase.	dimensionless	exp_id
tank_XX	Experimental tank number, where XX is equivalent to 1, 3, 8, 10, 12, 13, 14, 15 for experiment I, and 1, 2, 7, 8, 10, 14, 15, 16 for experiment II.	dimensionless	tank
laboratory	Laboratory where experiments were conducted.	unitless	laboratory
lat	Latitude component of geographic position where experiments were conducted.	decimal degrees	lat
lon	Longitude component of geographic position where experiments were conducted.	decimal degrees	lon
pCO2_X	Partial pressure of CO2 for expeimental tank on day of measurement, where X is equal to I for experiment I and II for experiment II.	microatmospheres	pCO2
pH_X	pH (total scale) of expeimental tank on day of measurement, where X is equal to I for experiment I and II for experiment II.		pH
sal_X	Salinity of experimental tank on day of measurement, where X is equal to I for experiment I and II for experiment II.	PSU	sal
TA_X	Total alkalinity of experimental tank on day of measurement, where X is equal to I for experiment I and II for experiment II.	umol per kilogram	TALK
temp_X	Water temperature of experimental tank on day of measurement, where X is equivalent to I for experiment I or II for experiment II.	degrees Celsius	temp
yrday	Year day of tank water sampling.		yrday

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Dataset: recruit_seawater_chemistry