Metadata for field dilution experiments to measure community microzooplankton grazing rates in Kaneohe Bay, HI from 2012-2013 (EAGER: Copepod nauplii project)
Project
| Contributors | Affiliation | Role |
|---|---|---|
| Goetze, Erica | University of Hawaii at Manoa (SOEST) | Principal Investigator |
| Lenz, Petra H. | University of Hawaii at Manoa (SOEST) | Co-Principal Investigator |
| Selph, Karen E. | University of Hawaii at Manoa (SOEST) | Co-Principal Investigator |
| Copley, Nancy | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
Abstract
Phytoplankton Rates: µ0, µN, m (gross growth rate, nutrient-amended gross growth rate, and mortality rate, respectively): Phytoplankton growth and mortality rates were determined with seawater dilution experimental manipulations of freshly collected surface seawater as originally described in Landry & Hassett (1982), with modifications as described by Selph et al. (2005).
BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- reformatted date from d-Mon-yy to yyyy-mm-dd
- replaced spaces with underscores
- added lat/lon columns
| File |
|---|
growth_KB.csv (Comma Separated Values (.csv), 734 bytes) MD5:6a687ea6221c5e37a0a4f5f9b80c08ba Primary data file for dataset ID 637670 |
| Parameter | Description | Units |
| KBG_expt | experiment id | unitless |
| date_local | local date | yyyy-mm-dd |
| lat | latitude; north is positive | decimal degrees |
| lon | longitude; east is positive | decimal degrees |
| chla_init | initial chlorophyll-a concentration | micrograms/liter |
| growth_init | gross growth rate | per day |
| mortality | mortality rate | per day |
| r_squared | the coefficient of determination | unitless |
| growth_nuts | nutrient-amended gross growth rate | per day |
| comment | comments | unitless |
| Dataset-specific Instrument Name | |
| Generic Instrument Name | Fluorometer |
| Generic Instrument Description | A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. |
| Dataset-specific Instrument Name | |
| Generic Instrument Name | Flow Cytometer |
| Generic Instrument Description | Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) |
Goetze_2012-2013
| Website | |
| Platform | lab UHawaii_SOEST |
| Start Date | 2012-03-16 |
| End Date | 2013-06-05 |
| Description | microzooplankton studies |
EAGER: New molecular methods for studying copepod nauplii in the field (EAGER: Copepod nauplii)
Description from NSF Award Abstract:
The most abundant metazoans in the open sea are often the earliest developmental stages of copepods, their nauplii. Nauplii remain under-studied due to the limitations of conventional techniques and an historical emphasis on studying the larger mesozooplankton. However, there is increasing recognition that nauplii play important roles in food web dynamics, and considerable evidence that nauplii may be important trophic intermediaries between microbial and classical food webs due to their high abundance, high weight-specific ingestion rates, and ability to feed on relatively small particles. This team of investigators is developing a novel molecular approach to studying diverse populations of nauplii in mixed field samples based on quantitative Polymerase Chain Reaction (qPCR). They propose to complete development and validation of this qPCR-based technique for enumeration of nauplii, and evaluate its utility in the field. The specific objectives of this research are to identify and reduce technical and biological sources of error in the methodology, determine the accuracy of the method across a range of environmental conditions, and complete one paired field experiment that compares the grazing impact of naupliar and protozoan micro-grazers in a model subtropical coastal ecosystem.
Note: This project is funded by an NSF EAGER award.
Related publications:
Jungbluth, M.J., Goetze, E., and Lenz, P.H. 2013. Measuring copepod naupliar abundance in a subtropical bay using quantitative PCR. Marine Biology, 160: 3125-3141. doi: 10.1007/s00227-013-2300-y
Jungbluth, M.J., and Lenz, P.H. 2013. Copepod diversity in a subtropical bay based on a fragment of the mitochondrial COI gene. Journal of Plankton Research, 35(3): 630-643. doi: 10.1093/plankt/fbt015
| Funding Source | Award |
|---|---|
| NSF Division of Ocean Sciences (NSF OCE) |
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