|Goetze, Erica||University of Hawaii at Manoa (SOEST)||Principal Investigator|
|Lenz, Petra||University of Hawaii at Manoa (SOEST)||Co-Principal Investigator|
|Selph, Karen E.||University of Hawaii at Manoa (SOEST)||Co-Principal Investigator|
|Copley, Nancy||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
These data report initial and final measurements of prey abundances in bottle incubation experiments to measure naupliar grazing.
All samples were collected from surface water in Southern Kaneohe Bay, Oahu, Hawaii (21°25’56.7”N, 157°46’47.1”W).
Pro, Syn, Peuk, PE+Peuk, HBact data: These data were collected and analyzed by flow cytometry as described in Selph et al. (2011). Briefly, samples were preserved (0.05% paraformaldehyde, final concentration), and frozen to -80°C within 1 h. Batches of samples were thawed, stained with Hoechst 34442 (1 µg/mL final concentration) and analyzed on a flow cytometer (Beckman Coulter Altra) with dual laser excitation (UV range and 488 nm, 200 mW and 1 W, respectively), mated to a Harvard Apparatus syringe pump for quantitative delivery (100 µl at 50 µl min-1). Resulting listmode files were analyzed off-line using FlowJo software (Treestar). Populations determined, based on red fluorescence (chlorophyll, 680±20 nm), orange fluorescence (phycoerythrin, 575±20 nm), blue fluorescence (DNA, 450±40 nm), and light scatter signals (forward and 90°). Distinguishable populations were Prochlorococcus, Synechococcus, Photosynthetic Eukaryotes (PEUK), High phycoerythrin-containing PEUK (PE+Peuk), and non-pigmented prokaryotes (a.k.a. heterotrophic bacteria, HBACT).
- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
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|abund_PE_Peuk||high PhycoErythrin-containing Photosynthetic eukaryotes||cells/mL|
|abund_Hbact||non-pigmented prokaryotes (Heterotrophic bacteria)||cells/mL|
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Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
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Description from NSF Award Abstract:
The most abundant metazoans in the open sea are often the earliest developmental stages of copepods, their nauplii. Nauplii remain under-studied due to the limitations of conventional techniques and an historical emphasis on studying the larger mesozooplankton. However, there is increasing recognition that nauplii play important roles in food web dynamics, and considerable evidence that nauplii may be important trophic intermediaries between microbial and classical food webs due to their high abundance, high weight-specific ingestion rates, and ability to feed on relatively small particles. This team of investigators is developing a novel molecular approach to studying diverse populations of nauplii in mixed field samples based on quantitative Polymerase Chain Reaction (qPCR). They propose to complete development and validation of this qPCR-based technique for enumeration of nauplii, and evaluate its utility in the field. The specific objectives of this research are to identify and reduce technical and biological sources of error in the methodology, determine the accuracy of the method across a range of environmental conditions, and complete one paired field experiment that compares the grazing impact of naupliar and protozoan micro-grazers in a model subtropical coastal ecosystem.
Note: This project is funded by an NSF EAGER award.
Jungbluth, M.J., Goetze, E., and Lenz, P.H. 2013. Measuring copepod naupliar abundance in a subtropical bay using quantitative PCR. Marine Biology, 160: 3125-3141. doi: 10.1007/s00227-013-2300-y
Jungbluth, M.J., and Lenz, P.H. 2013. Copepod diversity in a subtropical bay based on a fragment of the mitochondrial COI gene. Journal of Plankton Research, 35(3): 630-643. doi: 10.1093/plankt/fbt015