Dataset: respiration and photosynthesis II

Name: Biomass-normalized dark respiration and net photosynthesis rates in coral and algae as a function of pCO2 where LEDR (light-enhanced dark respiration) was measured (OA coral adaptation project)
Published: 2016-05-11
Keywords: biota, oceans

Cite This Dataset

Spatial Extent: N:-17.4907 E:-149.826 S:-17.4907 W:-149.826

Project:

Collaborative Research: Ocean Acidification and Coral Reefs: Scale Dependence and Adaptive Capacity (OA coral adaptation)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Co-Principal Investigator:

Robert Carpenter (California State University Northridge, CSUN)

Peter J. Edmunds (California State University Northridge, CSUN)

Contact:

Peter J. Edmunds (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2016-05-11

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Biomass-normalized dark respiration and net photosynthesis rates in coral and algae as a function of pCO2 where LEDR (light-enhanced dark respiration) was measured (OA coral adaptation project)

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Description

Related Reference: Comeau, S., Carpenter, R.C., Edmunds, P.J. Effects of pCO2 on photosynthesis and respiration of tropical scleractinian corals and calcified algae. ICES Journal of Marine Science doi:10.1093/icesjms/fsv267. Figures 3 and 4.

Methods & Sampling

Changes in dissolved oxygen in coral and algal samples were monitored during 30 min incubations with PreSens dipping oxygen optodes (PSt3) connected to Fibox 3 transmitters (Precision Sensing GmbH, Germany). The oxygen probes were calibrated every morning using a 2-point calibration in water-saturated air (100%) and anoxic seawater created by supersaturating seawater with sodium dithionite (Na2S2O4). Temperature was held constant during incubations by circulating water in a water jacket surrounding the incubation chamber. Chambers were positioned on magnetic stir plates, and seawater flow inside the chambers was regulated with magnetic stir bar that created vigorous and turbulent water motion. A similar irradiance to that used during incubations (i.e. 700 mmol quanta m22 s21) was maintained while the organisms were in the acrylic chambers by placing an LED lamp (75-W, Sol LED Module, Aquaillumination) above the chambers. On the same day that net photosynthesis was measured, and after at least 2 h of darkness (i.e. after 20:00 h), dark respiration was measured using an identical procedure to that used for photosynthesis, except the chamber was kept in darkness.

Data Processing Description

An Akaike information criterion (AIC) approach was used to determine if linear, logarithmic, or polynomial models best described the functional relationships of dark respiration, LDER, net photosynthesis, and gross photosynthesis against pCO2 for each species (see details in Comeau et al., 2013)

Comeau, S., Edmunds, P. J., Spindel, N. B., and Carpenter, R. C. 2013. The responses of eight coral reef calcifiers to increasing partial pressure of CO2 do not exhibit a tipping point. Limnology andOceanography, 58: 388–398.

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- added site, lab, lat, lon columns

Data Files

File
resp_photo_II_sort.csv (Comma Separated Values (.csv), 28.51 KB) MD5:a783e92afa507b30bf72d2d41a68ceb9 Primary data file for dataset ID 648416

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1415268

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
MCR_Edmunds		2010-01-01	Richard B Gump Research Station - Moorea LTER	Peter J. Edmunds (Principal Investigator)	data info

Instruments

Automatic titrator

Supplied Name:

Supplied Description:

open cell, potentiometric titrations (automatic titrator T50, Mettler-Toledo)

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Conductivity Meter

Supplied Name:

Supplied Description:

YSI 3100

Instrument Type

Generic Name: Conductivity Meter

Generic Description:

Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.

LI-COR LI-193 PAR Sensor

Supplied Name:

Supplied Description:

4-pi quantum sensor LI-193 and a LiCor LI-1400 meter data logger.

Instrument Type

Generic Name: LI-COR LI-193 PAR Sensor

Acronym: LI-COR LI-193 PAR

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0458/

Generic Description:

The LI-193 Underwater Spherical Quantum Sensor uses a Silicon Photodiode and glass filters encased in a waterproof housing to measure PAR (in the 400 to 700 nm waveband) in aquatic environments. Typical output is in micromol s-1 m-2. The LI-193 Sensor gives an added dimension to underwater PAR measurements as it measures photon flux from all directions. This measurement is referred to as Photosynthetic Photon Flux Fluence Rate (PPFFR) or Quantum Scalar Irradiance. This is important, for example, when studying phytoplankton, which utilize radiation from all directions for photosynthesis. LI-COR began producing Spherical Quantum Sensors in 1979; serial numbers for the LI-193 begin with SPQA-XXXXX (licor.com).

Optode

Supplied Name:

Supplied Description:

PreSens dipping oxygen optodes (PSt3) connected to Fibox 3 transmitters (Precision Sensing GmbH, Germany).

Instrument Type

Generic Name: Optode

Generic Description:

An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.

pH Sensor

Supplied Name:

Supplied Description:

Orion, 3-stars mobile mounted with a MettlerDG115-SCpHelectrode

Instrument Type

Generic Name: pH Sensor

Generic Description:

An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
site	location of experiments	unitless	site
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
taxon	taxonomic group	unitless	taxon
species	species name	unitless	species
pCO2	Partial pressure of carbon dioxide in the water body	uatm	pCO2
dark_respi	dark respiration	ug O2/h/g	dark_O2_respir
Net_Production	net production	ug O2/h/g	net_O2
light_respi	light respiration	ug O2/h/g	O2_respir
photosynthesis	rate of net photosynthesis normalized to biomass	ug O2/h/g	photosynthesis
dark_respi_biomass	dark respiration - normalized for biomass	ug O2/h/g	dark_O2_respir
net_production_biomass	net production - normalized for biomass	ug O2/h/g	net_O2
light_respi_biomass	light respiration - normalized for biomass	ug O2/h/g	O2_respir
photosynthesis_biomass	photosynthesis rate - normalized for biomass	ug O2/h/g	no_bcodmo_term

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Dataset: respiration and photosynthesis II