Dataset: UConn Seasonal IPT

Name: Sets of sediment cores taken for isotope pairing and other nitrogen cycle parameters seasonally since November 2013 from two oyster farm and control sites in Rhode Island and New York
Published: 2016-10-14
Keywords: oceans

View Data: Data not available yet

Cite This Dataset

Project:

Microbial Regulation of Greenhouse Gas N2O Emission from Intertidal Oyster Reefs (Oyster Reef N2O Emission)

Principal Investigator:

Bongkeun Song (Virginia Institute of Marine Science, VIMS)

Co-Principal Investigator:

Craig Tobias (University of Connecticut, UConn - Avery Point)

Contact:

Amanda Vieillard (University of Connecticut, UConn - Avery Point)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

14 October 2016

Version Date:

2016-10-14

Restricted:

Yes

Release Date:

2019-03-01

Validated:

Current State:

Final with updates expected

Sets of sediment cores taken for isotope pairing and other nitrogen cycle parameters seasonally since November 2013 from two oyster farm and control sites in Rhode Island and New York

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Description

Sets of sediment cores taken for isotope pairing and other nitrogen cycle parameters seasonally since November 2013 from two oyster farm and control sites.

Status: Data are restricted from public access until 08 November 2017. Please contact the PI for prior access.

Methods & Sampling

Samples were collected at coastal oyster farms in Rhode Island (41 22' 45" N, 71 38' 43" W) and New York (41 16' 9" N, 71 59' 29" W). At both locations, the approximate depth is 2 meters.

Coupled direct denitrification (DNF), direct DNF, and dissimilatory nitrate reduction to ammonium (DNRA) are measured in sediments collected from each site in areas that are influenced by oysters and areas that are not. Seasonally five intact sediment cores are taken from directly underneath oyster growth and from control sites in both farms. Cores are incubated at in situ temperature with no air headspace and 15N-NO3- tracer added to the overlying water. Each core is sacrificed and slurried as a single time point. Water column dissolved inorganic nitrogen (DIN) samples are analyzed colorimetrically for concentration (WestCo SmartChem). N2 isotopologues are measured on the membrane inlet mass spectrometer (MIMS) and are used to calculate rates of both coupled and direct DNF via the isotope pairing method (IPT, Steingruber et al. 2001). Sediment bound 15N-NH4+ enrichment is measured using the azide hypobromide method (Zhang et al. 2007) and are run on the isotope ratio mass spectrometer (IRMS). 15N-NH4+ enrichment is then used to calculate rates of DNRA. Additionally, sediment sub-samples are run on an elemental analyzer (EA) coupled to IRMS for sediment C:N, 15N, and 13C. Finally, in order to constrain the overall 15N mass balance intermediate pools such as NO2 and N2O are also analyzed.

Related references:
Steingruber, S. M., Friedrich, J., Gächter, R., & Wehrli, B. 2001. Measurement of denitrification in sediments with the 15N isotope pairing technique. Applied and Environmental Microbiology, 67(9), 3771-3778. doi:10.1128/AEM.67.9.3771-3778.2001

Zhang, L., M. A. Altabet, T. Wu, O. Hadas. 2007. Sensitive measurement of NH4+ 15N/14N (15NH4+) at natural abundance levels in fresh and salt waters. Analytical Chemistry 79:5297-5303. doi:10.1021/ac070106d

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1233372

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
UConn_Tobias_2013-14		2013-10-25	Univ_Connecticut	Craig Tobias (Principal Investigator)	data info

Instruments

Discrete Analyzer

Supplied Name: WestCo SmartChem

Supplied Description:

Water column DIN samples are analyzed colorilmetrically for concentration (WestCo SmartChem).

Instrument Type

Generic Name: Discrete Analyzer

Generic Description:

Discrete analyzers utilize discrete reaction wells to mix and develop the colorimetric reaction, allowing for a wide variety of assays to be performed from one sample. These instruments are ideal for drinking water, wastewater, soil testing, environmental and university or research applications where multiple assays and high throughput are required.

Isotope-ratio Mass Spectrometer

Supplied Name: isotope ratio mass spectrometer

Supplied Description:

Sediment bound 15N-NH4+ enrichment is measured using the azide hypobromide method (Zhang et al. 2007) and are run on the isotope ratio mass spectrometer (IRMS).

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Membrane Inlet Mass Spectrometer

Supplied Name: membrane inlet mass spectrometer

Supplied Description:

N2 isotopologues are measured on the membrane inlet mass spectrometer (MIMS) and are used to calculate rates of both coupled and direct DNF via the isotope pairing method (IPT, Steingruber et al. 2001).

Instrument Type

Generic Name: Membrane Inlet Mass Spectrometer

Acronym: MIMS

Generic Description:

Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
date	Date of sampling formatted as yyyy-mm-dd	unitless	date
season	Season of sampling	unitless	season
location	Sampling location name	unitless	site
state	State of sampling location (NY or RI)	unitless	site
lat	Latitude of sampling location	decimal degrees	lat
lon	Longitude of sampling location	decimal degrees	lon
site	Site (Oyster or Control)	unitless	site
N2O_flux	N2O flux	micromoles per square meter per hour (umol m-2 h-1)	no_bcodmo_term
NO2_flux	NO2 flux	micromoles per square meter per hour (umol m-2 h-1)	no_bcodmo_term
NO3_flux	NO3 flux	micromoles per square meter per hour (umol m-2 h-1)	no_bcodmo_term
sediment_15N	Sediment 15N	micromoles (umol)	no_bcodmo_term
pcnt_OM	Percent organic matter	percent (%)	no_bcodmo_term
gC_per _gsed	grams of Carbon per grams of sediment	g C / g sediment	no_bcodmo_term
d13C	d13C	per mil	d13C
d15N	d15N	per mil	no_bcodmo_term
mgN_per_gsed	milligrams of Nitrogen per grams of sediment	mg N / g sediment	no_bcodmo_term
C_to_N	C:N	dimensionless (ratio)	C_to_N

Status message

Database

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Dataset: UConn Seasonal IPT