Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Instruments
• Project Information
• Funding

Detection limits of the results of HPLC – derived photopigment concentrations for bioassays.

These are the detection limits for this dataset: HPLC Photopigment Data for Bioassays

Methodology from Pickney, J.L.: The USC HPLC Method - Technical Description.pdf

BCO-DMO Data Processing Notes:

-Reformatted column names to comply with BCO-DMO standards.
-Data were originally separated by month (September and November) into two spreadsheets. Data were combined into one file and the columns "month" and "year" were added.
-Colors and headers were removed.

Phytoplankton, traditionally viewed as primary producers at the base of aquatic food webs, provide an energy source for higher trophic levels. However, some phytoplankton species function as both primary producers and heterotrophic secondary consumers. Phytoplankton that are photosynthetically competent but also take up and assimilate organic compounds are classified as facultative mixotrophs or, more simply, photomixotrophs. Unfortunately, we currently have few estimates of the proportion of the phytoplankton community that function as photomixotrophs, their rate of secondary production, or their temporal variation in abundance. Current paradigms about trophodynamics in marine systems do not consider this potentially important alternative pathway for energy flow for phytoplankton. The implication is that we may be missing a significant, fundamental process that affects carbon cycling and trophodynamics in estuarine systems. Furthermore, changes in the DOC composition due to anthropogenic alterations may result in changes in phytoplankton community structure and possibly promote the proliferation of harmful algal bloom species. In terms of ecosystem function, even moderate rates of photomixotrophy could potentially alter our current understanding of phytoplankton productivity, overall C turnover, competitive interactions, and energy transfer in estuarine environments. This project will use a novel approach to provide quantitative measures of the in situ rates and magnitudes of facultative heterotrophy in natural, estuarine phytoplankton communities over seasonal time scales in a representative estuarine ecosystem. The project will utilize a unique 14C radiolabeling technique to quantify the in situ assimilation rates of DOC by estuarine photomixotrophs and estimate the amount of DOC converted to phytoplankton biomass by photomixotrophy over seasonal time scales. This information will provide new insights into carbon dynamics in estuaries, the contribution of DOC to estuarine food webs, and the importance of photomixotrophy in determining the structural and functional characteristics of estuarine phytoplankton communities.