Dataset: HPLC Photopigment Data for Bioassays - Detection Limits

Name: Detection limits for the results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project)
Published: 2017-07-28
Keywords: oceans

Cite This Dataset

Spatial Extent: N:33.452783 E:-79.071852 S:33.091684 W:-79.318359

Project:

Assimilation rates of dissolved organic carbon by photomixotrophic estuarine phytoplankton (Photomixotrophy)

Principal Investigator:

James L. Pinckney (University of South Carolina at Columbia)

BCO-DMO Data Manager:

Hannah Ake (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Final

Version Date:

2017-07-28

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Detection limits for the results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project)

Abstract:

Detection limits for the results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project)

Expand/Collapse All

Description

Detection limits of the results of HPLC – derived photopigment concentrations for bioassays.

Methods & Sampling

These are the detection limits for this dataset: HPLC Photopigment Data for Bioassays

Data Processing Description

Methodology from Pickney, J.L.: The USC HPLC Method - Technical Description.pdf

BCO-DMO Data Processing Notes:

-Reformatted column names to comply with BCO-DMO standards.
-Data were originally separated by month (September and November) into two spreadsheets. Data were combined into one file and the columns "month" and "year" were added.
-Colors and headers were removed.

Data Files

File
detection_limits.csv (Comma Separated Values (.csv), 23.35 KB) MD5:9b6c2216d2fc8ea2661d458e8c5e54f2 Primary data file for dataset ID 710183

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1260134

Deployments

None available yet

Instruments

High-Performance Liquid Chromatograph

Supplied Name: Temperature-controlled autosampler (Shimadzu SIL10-A vp) with a 500 µl injection loop

Supplied Description:

Used in HPLC Analysis

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

High-Performance Liquid Chromatograph

Supplied Name: Photodiode array detector (PDA, Shimadzu SPD-M10A vp; 200 to 800 nm range)

Supplied Description:

Used in HPLC analysis

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

Liquid Scintillation Counter

Supplied Name: Flow Scintillation Counter (Packard Radiomatic 525a, 500 ul counting cell)

Supplied Description:

Used to measure chl a

Instrument Type

Generic Name: Liquid Scintillation Counter

Acronym: LSC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/

Generic Description:

Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.

Pump

Supplied Name: Binary Gradient Pump (Shimadzu dual LC10-AT vp and Controller SCL-10A vp)

Supplied Description:

Used in HPLC analysis

Instrument Type

Generic Name: Pump

Generic Description:

A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Year	Year sample was taken; YYYY	unitless	year
Month	Month sample was taken	unitless	no_bcodmo_term
Sample_Number	Sample number	unitless	no_bcodmo_term
Chl_LOD	Chlorophyll c1+c2 effective limit of detection	micrograms per liter	chl_c1_c2
Chl_LOQ	Chlorophyll c1+c2 effective limit of quantification	micrograms per liter	chl_c1_c2
Perid_LOD	Peridinin effective limit of detection	micrograms per liter	peridinin
Perid_LOQ	Peridinin effective limit of quanitification	micrograms per liter	peridinin
ButFuc19_LOD	19' Butanoyloxyfucoxanthin effective limit of detection	micrograms per liter	fucox_but
ButFuc19_LOQ	19' Butanoyloxyfucoxanthin effective limit of quantification	micrograms per liter	fucox_but
Fuco_LOD	Fucoxanthin effective limit of detection	micrograms per liter	fucox
Fuco_LOQ	Fucoxanthin effective limit of quantification	micrograms per liter	fucox
HexFuc19_LOD	19' Hexanoyloxyfucoxathin effective limit of detection	micrograms per liter	fucox_hex
HexFuc19_LOQ	19' Hexanoyloxyfucoxathin effective limit of quantification	micrograms per liter	fucox_hex
Neo_LOD	Neoxanthin effective limit of detection	micrograms per liter	neox
Neo_LOQ	Neoxanthin effective limit of quantification	micrograms per liter	neox
Viola_LOD	Violaxanthin effective limit of detection	micrograms per liter	violax
Viola_LOQ	Violaxanthin effective limit of quantification	micrograms per liter	violax
Diad_LOD	Diatoxanthin effective limit of detection	micrograms per liter	diatox
Diad_LOQ	Diatoxanthin effective limit of quantification	micrograms per liter	diatox
Allox_LOD	Alloxanthin effective limit of detection	micrograms per liter	allox
Allox_LOQ	Alloxanthin effective limit of quantification	micrograms per liter	allox
Diat_LOD	Diatoxanthin effective limit of detection	micrograms per liter	diatox
Diat_LOQ	Diatoxanthin effective limit of quantification	micrograms per liter	diatox
Lutein_LOD	Lutein effective limit of detection	micrograms per liter	lutein
Lutein_LOQ	Lutein effective limit of quantification	micrograms per liter	lutein
Zeax_LOD	Zeaxanthin effective limit of detection	micrograms per liter	pigment_conc
Zeax_LOQ	Zeaxanthin effective limit of quantification	micrograms per liter	pigment_conc
Gyro_LOD	Gyroxanthin effective limit of detection	micrograms per liter	gyrox
Gyro_LOQ	Gyroxanthin effective limit of quantification	micrograms per liter	gyrox
Chl_b_LOD	Chlorophyll b effective limit of detection	micrograms per liter	chl_b
Chl_b_LOQ	Chlorophyll b effective limit of quantification	micrograms per liter	chl_b
Chl_a_LOD	Chlorophyll a effective limit of detection	micrograms per liter	chl_a
Chl_a_LOQ	Chlorophyll a effective limit of quantification	micrograms per liter	chl_a

Status message

Database

Contribute Data

Dataset: HPLC Photopigment Data for Bioassays - Detection Limits