Dataset: Photosynthesis-Irradiance curve (P-E)

Name: Photosynthesis-Irradiance curve (P-E) raw data for coral Orbicella faveolata from Varadero and Rosario reef sites, Colombia, Oct. 2016 and May 2017 (Varadero Reef project)
Published: 2018-03-05
Keywords: biota, oceans

Cite This Dataset

Spatial Extent: N:10.3028 E:-75.5819 S:10.3028 W:-75.5819

Temporal Extent: 2016-10 - 2017-05

Project:

RAPID: Coral robustness: lessons from an "improbable" reef (Varadero Reef)

Principal Investigator:

Mónica Medina (Pennsylvania State University, PSU)

Co-Principal Investigator:

Roberto Iglesias-Prieto (Pennsylvania State University, PSU)

Contact:

Tomás Lopez Lodoño (Pennsylvania State University, PSU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-03-05

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Photosynthesis-Irradiance curve (P-E) raw data for coral Orbicella faveolata from Varadero and Rosario reef sites, Colombia, Oct. 2016 and May 2017 (Varadero Reef project)

Abstract:

This dataset contains the results of the photosynthetic parameters calculated based on the analysis of the P-E curves from coral fragment of the species Orbicella faveolata used in the transplant experiment between three sites: Varadero (10°18'23.3"N, 75°35'08.0"W), Rosario (10°11'12.1"N, 75°44'43.0"W) and Abanico (10°18'5.80"N, 75°34'37.10"W). The tag number/color of each fragment, the date of data collection, and the sites of origin and destination are specified.

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Description

This dataset contains the raw data from the Photosynthesis-Irradiance (P-E) curves for each coral Orbicella faveolata from Varadero and Rosario Reef sites.

Related Reference:
Pizarro V, Rodríguez SC, López-Victoria M, Zapata FA, Zea S, Galindo-Martínez CT, Iglesias-Prieto R, Pollock J, Medina M. (2017) Unraveling the structure and composition of Varadero Reef, an improbable and imperiled coral reef in the Colombian Caribbean. PeerJ 5:e4119 https://doi.org/10.7717/peerj.4119

Methods & Sampling

The Varadero Reef is located south-west of the Cartagena Bay close to the southern strait that connects the Bay to the Caribbean Sea in Colombia. The Bay is a receiving estuary from the Magdalena River through the Canal del Dique, a man-made channel whose construction and operation dates back almost a century. The depth of the particular transplant site in Varadero is 3.5m, while in Abanico, a nearby site with low coral cover, and Rosario, a site inside a marine protected area located south-west with relatively high coral cover, is 3m and 12m.

The P-E curves were constructed by measuring the consumption/evolution of oxygen by each coral fragment used in the transplant experiment after exposing them to different levels of increasing light intensity. A high precision fiber-optical sensor connected to a PC-based oxygen meter was used to measure the oxygen concentration at each light level in hermetic water-jacked chambers with filtered seawater (0.45µm) and controlled magnetic stirrers producing agitation. The temperature was maintained at 28°C. Dark respiration was calculated by averaging the oxygen consumption rate at the beginning (pre-illumination) and at the end (post-illumination) of each incubation. Photosynthetic descriptors were calculated from the P-E curves based on Iglesias-Prieto and Trench (1994) and Osinga et al. (2012).

The software Pyro Oxygen Logger (PyroScience GmbH, Germany) was used to manage the oximeter and data operations during incubations.

Methodology Reference:
- Iglesias-Prieto, R. & R.K. Trench. 1994. Acclimation and adaptation to irradiance in symbiotic dinoflagellates. I. Responses of the photosynthetic unit to changes in photon flux density. Mar Ecol Prog Ser 113: 163-175. Stable URL: http://www.jstor.org/stable/24849586.
- Osinga, R., R. Iglesias-Prieto and S. Enríquez. 2012. Measuring photosynthesis in symbiotic invertebrates: a review of methodologies, rates and processes. 220-256. In: Najafpour, M.N. (Ed.) Applied Photosynthesis. InTech. 422 p. Full text url: http://library.wur.nl/WebQuery/wurpubs/fulltext/245530

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- blank values were replaced with no data value 'nd'

Data Files

File
PE_raw_all_v2.csv (Comma Separated Values (.csv), 71.17 KB) MD5:6bb306aafd0632618f2bbfbd2098a458 Primary data file for dataset ID 719130

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1642311

Deployments

None available yet

Instruments

Light Meter

Supplied Name: cosine light sensor (Waltz)

Supplied Description:

Instrument Type

Generic Name: Light Meter

Generic Description:

Light meters are instruments that measure light intensity. Common units of measure for light intensity are umol/m2/s or uE/m2/s (micromoles per meter squared per second or microEinsteins per meter squared per second). (example: LI-COR 250A)

Oxygen Sensor

Supplied Name: Optical oxygen meter FireStingO2 (Pyroscience)

Supplied Description:

Used to measure oxygen evolution.

Instrument Type

Generic Name: Oxygen Sensor

Acronym: Dissolved Oxygen Sensor

Generic Description:

An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Date_collected	date collected	unitless	date
Light_integ	light intensity	micromol quanta/meter^2/second	irradiance
Light_Level	light level	unitless	no_bcodmo_term
Slope_O2_prod	rate of oxygen production curve	micromol O2/centimeter^2/hour	photosynthesis
Rate_O2_prod	rate of oxygen production was normalized per surface area	micromol O2/centimeter^2/hour	photosynthesis
Fragment_id	fragment number	unitless	sample
Chamber_channel	sample chamber identifier	unitless	sample
Parent_Colony_id	parent colony number	unitless	sample
Transplanted_from	location coral fragment was transplanted from	unitless	site
Transplanted_to	location coral fragment was transplanted to	unitless	site
Species	taxonomic species name	unitless	species
Parent_tag_color	parent colony tag color	unitless	tagid
Fragment_tag_color	fragment tag color	unitless	tagid

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Database

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Dataset: Photosynthesis-Irradiance curve (P-E)