|Phillips, Nicole||Victoria University of Wellington||Principal Investigator, Contact|
|Biddle, Mathew||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
These data are from an experiment that test the nutritional strategies of Ceraesignum (Dendropoma) maximum larvae. For additional datasets see related files.
In this experiment, larval growth and metamorposis was tested using different food levels.
Larvae hatched on October 10, 2009 and ~50 were distributed into each tubs on 500mL filtered sea water (FSW). Because the greatest metamorphic success was in the Isochrysis galbana treatment during experiment 1, only that species was used in experiment 2.
Three food densities were created high food (4 x 104 cells mL-1 ), low food (4 x 103 cells mL-1 ) plus an Unfed treatment in which larvae were raised in FSW. Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. Each of the three treatments had three replicate containers; 50 larvae were placed into each container initially, exept two containers that had 1 extra. Every 3 days for 9 days starting on October 4, 2009, larvae were counted in each container to quantify survival. Water and food were also replenished. On days 6 and 8, fifteen larvae were transferred for the settlement challenge experiment.
For days 3 and 6: percent survival = (number alive on day of sampling/ number alive initial )*100
For day 9: percent survival = (number alive day 9/number remaining on day 8)*100
|FOOD_LEVEL||High (HI) Low (LO) or Unfed treatments||unitless|
|number_alive_initial||the number larvae in treatments alive initially||unitless|
|number_alive_day_3||number of alive larvae found on day 3||unitless|
|number_dead_d3||number of dead larvae found on day 3||unitless|
|number_alive_d6||number of alive larvae found on day 6||unitless|
|number_dead_d6||number of dead larvae found on day 6||unitless|
|number_transferred_day_6||number of larvae transferred to settlement challenges||unitless|
|number_transferred_day_8||number of larvae transferred to settlement challenges on day 8||unitless|
|number_remain||the number of larvae still tubs after larvae for settlement challenges were removed||unitless|
|number_alive_d9||number of alive larvae found on day 9||unitless|
|number_dead_9||number of dead larvae found on day 9||unitless|
|percent_survival_day_3||survival from day 1 to 3||unitless (percent)|
|percent_survival_day_6||survival from day 3 to 6||unitless (percent)|
|percent_survival_day_9||survival from day 6 to 9||unitless (percent)|
|Dataset-specific Instrument Name|| |
|Generic Instrument Name|| |
|Dataset-specific Description|| |
Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment.
|Generic Instrument Description|| |
A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
Osenberg et al Moorea
|Start Date|| |
|End Date|| |
Description from NSF abstract:
Ecological surprises are most likely to be manifest in diverse communities where many interactions remain uninvestigated. Coral reefs harbor much of the world's biodiversity, and recent studies by the investigators suggest that one overlooked, but potentially important, biological interaction involves vermetid gastropods. Vermetid gastropods are nonmobile, tube-building snails that feed via an extensive mucus net. Vermetids reduce coral growth by up to 80%, and coral survival by as much as 60%. Because effects vary among coral taxa, vermetids may substantially alter the structure of coral communities as well as the community of fishes and invertebrates that inhabit the coral reef.
The investigators will conduct a suite of experimental and observational studies that: 1) quantify the effects of four species of vermetids across coral species to assess if species effects and responses are concordant or idiosyncratic; 2) use meta-analysis to compare effects of vermetids relative to other coral stressors and determine the factors that influence variation in coral responses; 3) determine the role of coral commensals that inhabit the branching coral, Pocillopora, and evaluate how the development of the commensal assemblage modifies the deleterious effects of vermetids; 4) determine how vermetid mucus nets affect the local environment of corals and evaluate several hypotheses about proposed mechanisms; and 5) assess the long-term implications of vermetids on coral communities and the fishes and invertebrates that depend on the coral.
Note: The Principal Investigator, Dr. Craig W. Osenberg, was at the University of Florida at the time the NSF award was granted. Dr. Osenberg moved to the University of Georgia during the summer of 2014 (current contact information).