Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)

Website: https://www.bco-dmo.org/dataset/732600
Data Type: experimental
Version: 2
Version Date: 2018-03-26

Project
» Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Program
» Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)
ContributorsAffiliationRole
Kubler, Janet E.California State University Northridge (CSUN)Principal Investigator
Dudgeon, SteveCalifornia State University Northridge (CSUN)Co-Principal Investigator
Copley, NancyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager

Abstract
This dataset includes growth rates of Ulva measured at three weekly intervals as well as integrated over the experimental trial grown in culture under various pCO2 levels from May through July 2015.


Coverage

Spatial Extent: N:34 E:-118 S:33 W:-119
Temporal Extent: 2015-05-11 - 2015-07-27

Dataset Description

This dataset includes growth rates of Ulva measured at three weekly intervals as well as integrated over the experimental trial grown in culture under various pCO2 levels from May through July 2015.

Related Datasets:
Ulva: Carbonate chemistry pCO2: Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
Ulva: Chl a: Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
Ulva: CHN and stable isotopes: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)
Ulva: pH and temperature time-series: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)
Ulva: pH Drift: Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)
Ulva: Photosynthesis and respiration: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)


Methods & Sampling

Growth rate was measured in batch culture by pooling all plant thalli and summing them to attain the datum for the culture pot. Hence, the culture pot is the unit of replication. Growth rate was measured by recording the fresh weight of living material approximately every 6 days. Plant tissue was lightly stacked and gently pressed between layers of Kimwipes. This method reduced handling time and removed superficial water prior to weighing. The stack was then weighed together to attain the fresh weight for the pot. Relative growth rate was then calculated following Lüning (1990):  RGR(% . day-1 = (100*Ln(W2/W1))/(t2-t1), where W1, W2, t1, t2 correspond to fresh weights at times 1 (t1) and 2 (t2), respectively. The weekly relative growth rates track the dynamics of growth and its variation during the experiment. In addition to growth rates estimated approximately weekly, estimates of overall net and absolute growth rates integrated across the 3-week experiment were also made using the initial and final starting masses. The difference between the overall net and absolute growth rate estimates is that the latter estimate includes small tissue fragments derived from whole thalli that occasionally appeared at the end of a weekly growth interval, but were thought to not be healthy viable fragments that would generate new growth in the subsequent interval so were discarded at the beginning of the next growth interval.

Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection


Data Processing Description

BCO-DMO Processing Notes:
- added a conventional header with dataset name and description, PI names, version date


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Data Files

File
Ulva_Growth_pCO2_var.csv
(Comma Separated Values (.csv), 3.55 KB)
MD5:914ea098b2e5b3c7e8dc0450ee5257c2
Primary data file for dataset ID 732600

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Related Publications

Lüning, K. (1990). Seaweeds: Their Environment, Biogeography, and Ecophysiology. John Wiley and Sons, Inc. 978-0471624349
Methods

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Parameters

ParameterDescriptionUnits
LabelTrial and culture pot number unitless
pCO2_avgAverage pCO2 partial pressure in seawater tanks microatmospheres (µatm)
pCO2_sdVariability of pCO2 partial pressure - standard deviation microatmospheres (µatm)
GP1iPot biomass at the beginning of the first growth interval grams
GP1fPot biomass at the end of the first growth interval grams
GP2iPot biomass at the beginning of the second growth interval grams
GP2fPot biomass at the end of the second growth interval grams
GP3iPot biomass at the beginning of the third growth interval grams
GP3fPot biomass at the end of the third growth interval grams
RGR_GP1Calculated relative growth rate for the 1st growth interval %/day
RGR_GP2Calculated relative growth rate for the 2nd growth interval %/day
RGR_GP3Calculated relative growth rate for the 3rd growth interval %/day
RGR_GP1to3_NetCalculated net growth integrated across experiment duration %/day
added_massSummed biomass of small fragments from thalli in each growth interval grams
RGR_GP1to3_AbsCalculated absolute growth integrated across experiment duration grams
Total_massSum of GP3f and added_mass grams


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Instruments

Dataset-specific Instrument Name
Mettler Toledo AG204 Delta Range Analytical Balance
Generic Instrument Name
scale
Generic Instrument Description
An instrument used to measure weight or mass.


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Project Information

Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Coverage: Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)


Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, Ulva spp. and Plocamium cartilagineum, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in Plocamium, which relies solely on diffusive uptake of CO2, and populations of Ulva spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of Ulva spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on Ulva spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.



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Program Information

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)


Coverage: global


NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).

In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.

Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.

PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)

NSF media releases for the Ocean Acidification Program:

Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification

Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?

Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)

Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)

Press Release 13-102 World Oceans Month Brings Mixed News for Oysters

Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)

Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants

Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)

Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)

Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)



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Funding

Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)

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