Dataset: Hard Substrates Isotopes, AT37-13 & AT42-03

Name: Averages and standard deviation across species for all macrofauna found on each carbonate rock collected during R/V Atlantis cruise AT37-13 in the Pacific margin of Costa Rica from May to June 2017
Published: 2021-02-17
Keywords: methane seep, deep sea, macrofauna, alvin, Costa Rica, continental slope, Tropical Pacific, d13C, d15N, hard substrate, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.747575.2

Spatial Extent: N:9.1306 E:-84.2132 S:8.30702 W:-84.8415

Temporal Extent: 2017-06-21 - 2018-11-05

Project:

Collaborative research: Quantifying the biological, chemical, and physical linkages between chemosynthetic communities and the surrounding deep sea (Costa Rica Seeps)

Principal Investigator:

Lisa A. Levin (University of California-San Diego, UCSD-SIO)

Co-Principal Investigator:

Gregory Rouse (University of California-San Diego, UCSD-SIO)

Student:

Olívia Soares Pereira (University of California-San Diego, UCSD-SIO)

Contact:

Lisa A. Levin (University of California-San Diego, UCSD-SIO)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-02-17

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Averages and standard deviation across species for all macrofauna found on each carbonate rock collected during R/V Atlantis cruise AT37-13 in the Pacific margin of Costa Rica from May to June 2017

Abstract:

Averages and standard deviation across species for all macrofauna found on each carbonate rock collected during R/V Atlantis cruise AT37-13 in the Pacific margin of Costa Rica from May to June 2017

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2021-02-17	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.747575.2	2021-04-22
2020-01-09	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.747575.1	2020-01-14

Description

Averages and standard deviation across species for all macrofauna found on each carbonate rock collected during AT37-13.

Methods & Sampling

Carbonate rocks were collected across seepage gradients at Mound 12 using HOV Alvin, and washed in miliQ water onboard. Before preserving, the sample was kept cold and animals were picked to sample tissue for isotope analysis. The tissue (aprox. 0.5 mg) was placed in pre weighted tin boats or combusted vials (500C overnight) and frozen at -70C. The remaining washed sample was sieved through 0.3 mm mesh, separating the sample in two fractions (a fine fraction with the meiofauna, and a coarser one with the macrofauna), both preserved in 96% Ethanol. In the laboratory, tissue samples were oven dried (60C) overnight, weighed and acidified with 12.5-25 ul 1N H3PO4 to remove inorganic C.

Data Processing Description

Stable isotope measurements (d13C and d15N) were carried out using a Costech elemental analyzer coupled to a Micromass Iso-prime isotope ratio mass spectrometer (EA/IRMS) at Washington State University.

BCO-DMO Processing:

modified parameter names;
replaced "-" with "nd" (no data)
replaced , with ; in substrate_number_and_dive column

Data Files

File
hard_substrate_isotopes.csv (Comma Separated Values (.csv), 13.61 KB) MD5:6c68daee3562c747471d9a29cd433aa1 Primary data file for dataset ID 747575

Supplemental Files

File
AT37-13_AT42-03_Raw_isotope_dataset filename: AT37-13_AT42-03_Raw_isotope_dataset_Feb16.xlsx (Octet Stream, 71.87 KB) MD5:986a99b8028cc3dd0c144b5a52111f92 Raw isotope dataset that is the base for the statistical (average and standard deviation) Hard Substrate Isotope dataset v2 (https://www.bco-dmo.org/dataset/747575)

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1634172

Deployments

Deployment	Start Date	Platform	Investigator
AT42-03_Alvin_Dives	2018-10-17	Alvin		data info
AT42-03	2018-10-17	R/V Atlantis	Erik E. Cordes (Chief Scientist)	data info
AT37-13_Alvin_Dives	2017-05-21	Alvin	Victoria J. Orphan (Principal Investigator)	data info
AT37-13	2017-05-20	R/V Atlantis	Erik E. Cordes (Chief Scientist)	data info

Instruments

Elemental Analyzer

Supplied Name: Costech elemental analyzer

Supplied Description:

Stable isotopes measurements (d13C and d15N) were carried out using a Costech elemental analyzer coupled to a Micromass Iso-prime isotope ratio mass spectrometer (EA/IRMS) at Washington State University.

Instrument Type

Generic Name: Elemental Analyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/

Generic Description:

Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.

Isotope-ratio Mass Spectrometer

Supplied Name: Micromass Iso-prime isotope ratio mass spectrometer

Supplied Description:

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise	Cruise ID, either AT37-13 or AT42-03	unitless	cruiseid
Site	Site name	unitless	site_descrip
Experiment	description	units	exp_type
Alvin_Dive_Sample_Number	Substrate number and Alivn dive from which the sample was collected	unitless	dive_id
Habitat	Seepage activity (active or inactive)	unitless	site_descrip
d13C_avg	Average of carbon isotope signature of animals on that substrate	per mil	d13C
d15N_avg	Average of nitrogen isotope signature of animals on that substrate	per mil	d15N
d13C_sd	Average of nitrogen isotope signature of animals on that substrate	per mil	d15N
d15N_sd	Standard deviation of nitrogen isotope signature of animals on that substrate	per mil	d15N
Latitude	Latitude of sampling location	decimal degrees	lat
Longitude	Longitude of sampling location	decimal degrees	lon

Status message

Database

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Dataset: Hard Substrates Isotopes, AT37-13 & AT42-03