Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Supplemental Files
• Related Publications
• Related Datasets
• Parameters
• Instruments
• Project Information
• Funding

We sampled 86 sites within seagrass habitats throughout Back Sound, North Carolina, USA (3442’ N to 3439’ N, 7637’ W to 7631’ W during July 2013. Sampling sites were located across 21 seagrass landscapes which were defined by 200m x 80m rectangles (matching common isolated bed size and shape within our system). These landscapes were previously selected to represent independent gradients in both total seagrass cover (260-11,764 m2) and landscape patchiness (1-75 individual patches; Yeager et al. 2016). Sampling sites in the current study were haphazardly placed across all 21 landscapes, but always located within seagrass itself, and not the unvegetated matrix. 

Seagrass core sampling and laboratory processing:
One core sample was taken from each sampling site. Each core measured 30 cm in diameter and captured the above ground seagrass habitat as well as the top 10 cm of the sediment surface. All cores were taken within 2 h of low tide and the GPS location of each core was marked with a Garmin 72H handheld unit (Garmin International, Olathe, Kansas, USA). Low-tide depth was measured in situ at each site at the time of sampling to the nearest 10 cm.  

Core samples were taken back to the lab and screened using a 500-m sieve; benthic macrofauna were sorted from larger debris and seagrass tissue and transferred to a 70% ETOH solution for storage. These organisms were then identified to the lowest taxon possible, enumerated, dried (48 h at 60 C), and weighed to the nearest 0.0001g. Only polychaetes with intact anterior appendages were identified and counted for density estimates to prevent overestimation and because these are requisite features for accurate identification. 

The core (30-cm diameter, PVC) was gently placed by hand at each site and pushed down to a constant depth of 10 cm into the sediment.  The core was gently rotated to break seagrass rhizomes, then dug out by hand, lifted, and placed into a resealable 1.5-gallon plastic bag. The sample was transported back to the laboratory on ice. 

BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* date value "41429" changed to "6/4/2013"
* date format converted to ISO 8601 standard format yyyy-mm-dd
* data values that were a period, indicating no value, changed to the default missing data identifier in BCO-DMO, "nd" meaning "no data."

Data version 2 (2019-06-18)
* species column names updated to correct spelling and unaccepted synonyms. Species name columns and associated species list include accepted correct spellings and associated aphiaIDs as of 2019-06-18. Source of species names and accepted synonyms is the World Register of Marine Species. Species names reviewed and any ambiguous names cleared up with feedback from the data submitter.
* Date for CP6 5 changed from 2015-07-16 to 2013-07-16

Amount and quality of habitat is thought to be of fundamental importance to maintaining coastal marine ecosystems. This research will use large-scale field experiments to help understand how and why fish populations respond to fragmentation of seagrass habitats. The question is complex because increased fragmentation in seagrass beds decreases the amount and also the configuration of the habitat (one patch splits into many, patches become further apart, the amount of edge increases, etc). Previous work by the investigators in natural seagrass meadows provided evidence that fragmentation interacts with amount of habitat to influence the community dynamics of fishes in coastal marine landscapes. Specifically, fragmentation had no effect when the habitat was large, but had a negative effect when habitat was smaller. In this study, the investigators will build artificial seagrass habitat to use in a series of manipulative field experiments at an ambitious scale. The results will provide new, more specific information about how coastal fish community dynamics are affected by changes in overall amount and fragmentation of seagrass habitat, in concert with factors such as disturbance, larval dispersal, and wave energy. The project will support two early-career investigators, inform habitat conservation strategies for coastal management, and provide training opportunities for graduate and undergraduate students. The investigators plan to target students from underrepresented groups for the research opportunities.

Building on previous research in seagrass environments, this research will conduct a series of field experiments approach at novel, yet relevant scales, to test how habitat area and fragmentation affect fish diversity and productivity. Specifically, 15 by 15-m seagrass beds will be created using artificial seagrass units (ASUs) that control for within-patch-level (~1-10 m2) factors such as shoot density and length. The investigators will employ ASUs to manipulate total habitat area and the degree of fragmentation within seagrass beds in a temperate estuary in North Carolina. In year one, response of the fishes that colonize these landscapes will be measured as abundance, biomass, community structure, as well as taxonomic and functional diversity. Targeted ASU removals will then follow to determine species-specific responses to habitat disturbance. In year two, the landscape array and sampling regime will be doubled, and half of the landscapes will be seeded with post-larval fish of low dispersal ability to test whether pre- or post-recruitment processes drive landscape-scale patterns. In year three, the role of wave exposure (a natural driver of seagrass fragmentation) in mediating fish community response to landscape configuration will be tested by deploying ASU meadows across low and high energy environments.