Dataset: 18O-labeled water

Name: Analyses of nitrate reference solutions in 18O-labeled water with the denitrifier method (Biological Nitrogen Isotope Fractionation project)
Published: 2021-11-29
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.865519.1

Temporal Extent: 2020-06-16 - 2020-08-19

Project:

CAREER: The biological nitrogen isotope systematics of ammonium consumption and production (Biological Nitrogen Isotope Fractionation)

Principal Investigator:

Julie Granger (University of Connecticut, UConn)

Student:

Mengyang Zhou (University of Connecticut, UConn)

Contact:

Mengyang Zhou (University of Connecticut, UConn)

Analyst:

Bonnie X. Chang (University of Washington, UW)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-29

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Analyses of nitrate reference solutions in 18O-labeled water with the denitrifier method (Biological Nitrogen Isotope Fractionation project)

Abstract:

IAEA-NO3 and USGS-34 reference solutions were supplemented with 18O-labeled water and analyzed for N and O isotope ratios.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2021-11-29	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.865519.1	2022-01-14

Methods & Sampling

Sampling and analytical procedures:

 Effects of sample volume and salinity on O atom exchange with water

NO3- reference materials (IAEA-NO3 and USGS-34) were diluted with mixtures of DIW and NO3--deplete Sargasso ranging in salinity from 0 to 35 ppt to achieve NO3- concentrations of 1, 3, 5 and 20 µmol L-1 – corresponding to respective injection volumes of 10, 3.3, 2 and 0.5 mL – in order to attain 10 nmoles of N analyte. Reference materials at corresponding salinity and NO3- concentrations were supplemented with 18O-labled water, resulting in δ18OH2O values ranging from -6.8 to 335‰ vs. VSMOW.

Data Processing Description

Processing notes from researcher:

Data were processed using Microsoft Excel

BCO-DMO processing notes

Date formats were changed from mm/dd/yy to yyyy-mm-dd
Spaces and units removed from column headers

Data Files

File
zhou_et_al_lab_data-6.csv (Comma Separated Values (.csv), 5.46 KB) MD5:4358ad4b6ff79bd5ab684820bc619a41 Primary data file for dataset ID 865519

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1554474

Deployments

None available yet

Instruments

Gas Chromatograph Mass Spectrometer

Supplied Name: Delta V Advantage continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA)

Supplied Description:

Delta V Advantage continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) interfaced with a modified Thermo Fisher Scientific Gas Bench sample preparation device fronted by dual cold traps (Casciotti et al., 2002) and a GC Pal autosampler (CTC Analytics, Zwingen, Switzerland) - to measure N and O isotope ratio of nitrate using the denitrified method.

Instrument Type

Generic Name: Gas Chromatograph Mass Spectrometer

Generic Description:

Instruments separating gases, volatile substances or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay by a mass spectrometer.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	Date of the experiments; yyyy-mm-dd Format: %Y-%m-%d	unitless	date
stdev_of_delta_18ON2O	Standard deviation of delta 18O replicates in each trial	unitless	delta18O
delta_18OH2O	Reference materials were supplemented with 18O-labaled water. Delta 18OH2O values (‰ vs. VSMOW) were calculated from dilution	‰ vs. VSMOW	delta18O
delta_18ON2O	O isotopic composition of nitrate measured with the denitrifier method using a Thermo Delta V GC-IRMS with modified Gas Bench II and a PAL autosampler	‰ vs. N2Otank	delta18O
Solution	Nitrate reference materials IAEA-NO3 and USGS-34	unitless	NO3
Concentration	Concentrations of nitrate reference solutions	umol L-1	NO3
Trial	Trial name	unitless	replicate
Salinity_in_vial	Nitrate reference materials (IAEA-NO3 and USGS-34) were diluted with a mixture of DIW and nitrate-deplete surface Sargasso seawater to difference salinity	ppt	sal_diff
Strain	The denitrifying bacteria strains used in the laboratory experiment: P. aureofaciens	unitless	species
Sample_volume	Sample volume injected to aliquot 10 nmol of nitrate	mL	volume

Database

Contribute Data

Dataset: 18O-labeled water