|Ferrón, Sara||University of Hawaii at Manoa (SOEST)||Principal Investigator, Contact|
|Soenen, Karen||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
Rates of gross oxygen production rates and total daytime oxygen consumption were measured using the 18O-water in vitro method and membrane inlet mass spectrometry as described by Ferrón et al. (2016).
Seawater samples for the incubations were collected in triplicate before dawn at six different depths (5, 25, 45, 75, 100, and 125m) with 12-L Niskin® bottles attached to a CTD rosette. Subsamples were taken from the Niskin bottles in acid-washed volume- calibrated 150-mLquartz glass bottles with ground-glass stoppers. The bottles were first rinsed with the seawater sample, and then filled from bottom to top using acid- washed silicon tubing, allowing the water to overflow at least twice the volume of the bottles. Before closing, the incubation bottles were spiked with 650 µL of 18O-H2O (97.2% 18O, Medical Isotopes) for the surface samples (5-45 m) and 1,000 µL for deeper samples (75-125 m). The samples were deployed in situ at the corresponding depths before dawn using a free-drifting array, and incubated until dawn. After recovery, the incubated samples were poisoned with saturated mercuric chloride solution to inhibit biological activity. Time-zero samples were also collected pre-dawn and poisoned at the time of the deployment.
Data is processed as described by Ferrón et al. (2016). Gross oxygen production is determined from the change in the 18O isotope ratio of dissolved oxygen over the incubation:
GOP = [R(O2)final - R(O2)initial / R(H2O) - R(O2)initial ] x [O2]initial
where R(O2)initial and R(O2)final are the initial and final isotope ratios (18O/16O) for dissolved oxygen, respectively, [O2]initial is the initial dissolved oxygen concentration, and R(H2O) is the 18O isotope ratio of the incubation water.
The net oxygen change during the incubation is simultaneously determined from the net change in oxygen to argon molar ratios:
NOC = [ ((O2/Ar)final / (O2/Ar)initial) - 1 ] x [O2]initial
where (O2/Ar)final and (O2/Ar)initial are the final and initial oxygen to argon molar ratios, respectively.
The total daytime oxygen consumption is derived as the difference between GOP and NOC and extrapolated to 24 hours (Ferrón et al., 2016).
BCO-DMO Processing Notes:
* Adjusted column names to comply with database requirements
* Added ISO8601 format of date and times
(Octet Stream, 12.57 KB)
Gross oxygen production km1910: Data table with mean and standard deviation of three replicates of parameters GOP and R.
|Cruise_ID||Cruise identification number||unitless|
|Date||Date of the incubations||UTC|
|Latitude||Array deployment latitude, south is negative||decimal degrees|
|Longitude||Array deployment Longitude, west is negative||decimal degrees|
|Time_in||Local time of array deployment in HST (UTC-10h)||unitless|
|Time_out||Local time of array recovery in HST (UTC-10h)||unitless|
|Depth||Sampling and incubation depth||meters|
|GOP||Gross oxygen production||mmol O2 m-3 d-1|
|R||Total daytime O2 consumption in the light||mmol O2 m-3 d-1|
|ISO_DateTime_UTC_In||Sampling start date and time (UTC) in ISO8601 format: YYYY-MM-DDThh:mmz||unitless|
|ISO_DateTime_UTC_Out||Sampling start date and time (UTC) in ISO8601 format: YYYY-MM-DDThh:mmz||unitless|
|Dataset-specific Instrument Name|| |
Membrane inlet mass spectrometer
|Generic Instrument Name|| |
Membrane Inlet Mass Spectrometer
|Dataset-specific Description|| |
Membrane inlet mass spectrometer consists of a Pfeiffer Vacuum HiCube 80 Eco turbo pumping station connected to a HiQuayTM quadrupole mass spectrometer (QMG700), with a Balzers radio frequency generator (QMH 400-5) and a Balzers analyzer (QMA 430). The membrane inlet design is from Bay Instruments (Easton, Maryland).
|Generic Instrument Description|| |
Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.
R/V Kilo Moana
|Start Date|| |
|End Date|| |
NSF Chief Scientist Training Cruise. For more information, see Rolling Deck to Repository (R2R): https://www.rvdata.us/search/cruise/KM1910 (cruise DOI: 10.7284/908380)
NSF Award Abstract:
The PIs request funds to provide training in leading and organizing research cruises to early career researchers in the areas of Biological and Chemical Oceanography. Participants in this training program would be introduced to pre-cruise planning and logistics, receive training in commonly used oceanographic sampling equipment, and conduct shipboard measurements during a 10-day oceanographic cruise to the North Pacific Subtropical Gyre (NPSG). The goal of this training program is to prepare early career scientists for leading and participating in interdisciplinary oceanographic research at sea.
The proposed program addresses the broader impacts criteria successfully. The research cruise and follow-up reports and publications focus on interdisciplinary questions important for advancing the field. Given the rapid changes that oceanic systems are undergoing, it is important to have a cadre of junior scientists who are adept at managing interdisciplinary collaborations and conducting research at sea. The PIs are considering ways to connect with diverse audiences in recruiting participants. The impact on early career oceanographers will be very strong. This will create an experience that will be a major impact on the careers of the trainees, especially if they stay in the oceanography field.