Water was collected at the reported time, and water collection was in nearly all cases taken on the incoming tide. Water was collected from a dock in a 1 liter (L) bottle, from which water temperature, salinity, and chlorophyll a was measured. Water samples for macronutrients were also taken from the 1 L sample bottle and filtered through a 0.45-micrometer (µm) filter and collected in duplicate and stored in 60-milliliter (mL) HDPE bottles at -20 degrees Celsius before being sent for analysis. Macronutrients (silicate, orthophosphate, ammonium, nitrite, and nitrate + nitrite) were measured from duplicate samples by the Center for Coastal Studies using a nutrient autosampler system. Duplicate 1 mL water samples were preserved with 0.125% EM grade glutaldehyde and frozen and stored at -80 degrees Celsius for flow cyometry analysis at a later time. Water temperature and salinity were measured with certified instruments. In situ chlorophyll a was measured by reading fluorescence of a whole water sample on a calibrated instrument.

Know Issues or Problems:
Sampling was done weekly in nearly all cases, and in most cases sampling was done on Wednesdays. There are a few exceptions and an additional sample on 2021-07-30 was taken two days after the 'normal' weekly sampling.