1b:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1c:T41b,

Water was collected at the reported time, and water collection was in nearly all cases taken on the incoming tide. Water was collected from a dock in a 1 liter (L) bottle, from which water temperature, salinity, and chlorophyll a were measured. Water samples for macronutrients were also taken from the 1 L sample bottle and filtered through a 0.45-micrometer (µm) filter and collected in duplicate and stored in 60-milliliter (mL) HDPE bottles at -20 degrees Celsius before being sent for analysis. Macronutrients (silicate, orthophosphate, ammonium, nitrite, and nitrate + nitrite) were measured from duplicate samples by the Center for Coastal Studies using a nutrient autosampler system. Duplicate 1 mL water samples were preserved with 0.125% EM grade glutaldehyde and frozen and stored at -80 degrees Celsius for flow cyometry analysis at a later time. Water temperature and salinity were measured with certified instruments. In situ chlorophyll a was measured by reading fluorescence of a whole water sample on a calibrated instrument.

1d:T5f5,Version History: Version 1: Version date: 2023-08-21 Description: This version contained data from 2021-03-10 to 2023-03-29. Additional data have been added in version 2 and important corrections were made to existing data. Notably, in version 1 the units for AirTemp were incorrectly reported as degrees Celsius and the data were offset by 2 weeks. BCO-DMO Processing for v1: - Imported original file named "RWU_cyanophage_timeseries_env_data.csv" into the BCO-DMO system. - Renamed fields to comply with BCO-DMO naming conventions (replaced "." with underscore). - Converted the original date/time field to ISO 8601 format (local time zone of EST/EDT). - Created a date/time field in ISO 8601 format in UTC time zone. - Removed the original date/time field. - Added columns for the sampling site latitude and longitude. - Saved the final file as "906562_v1_narragansett_bay_time-series.csv". Version 2: version date: 2025-04-03 Description: This version extends the dataset to include sampling dates of 2021-03-10 to 2023-09-27. Important corrections have also been made to existing data. Notably, in version 1 the units for AirTemp were incorrectly reported, and the data were offset by 2 weeks. Version 2 corrects these issues. BCO-DMO Processing for v2: - Imported original file "RWU_cyanophage_timeseries_env_data_v2_w_localtime.csv" to the BCO-DMO system. - Removed "NA" (missing data are empty/blank in the final csv file). - Saved the final file as "906562_v2_narragansett_bay_time-series.csv". 1e:T41b,This dataset includes basic water characteristics measured in Narragansett Bay (Rhode Island, USA) over two years as part of a study of the dynamics of marine cyanobacteria (Synechococcus) and viruses infecting cyanobacteria (cyanophage) in this estuarine ecosystem. The project principal investigators include Nathan Ahlgren (Clark University) and Marcie Marston (Roger Williams University), and the latter collected the data and water with the assistance of students and/or technicians. Water was sampled and measured once per week for a span of two and a half years. Water was sampled from a dock located on the campus of Roger Williams University (RWU) at an approximate latitude and longitude coordinates of 41.649737, -71.256378. Water was collected on an incoming tide in the morning and the following parameters were measured: water temperature, salinity, in situ chlorophyll a, nitrate + nitrite, phosphate, and silicate. Water samples were collected and preserved for measurement of Synechococcus abundance by flow cytometry at a later date.1f:Td40,NSF Award Abstract: Viral infection influences the flow of nutrients in the oceans and the diversity and structure of ecologically important microbial communities. Understanding which viruses infect which hosts is critical to understanding the exact impact of viruses, but there are still critical gaps in knowledge about how widely viruses can infect specific bacteria types and how this can change over time. This project includes the isolation and characterization of hundreds of co-occurring photosynthetic bacteria (cyanobacteria from the genus Synechococcus) and viruses that infect them (cyanophage) from Narragansett Bay, Rhode Island to assess the degree to which they can infect each other and identify specific genes that control cross-infection. DNA collected for over 10 years from Narragansett Bay is used to track Synechococcus and cyanophage communities to determine how virus-host interactions play out in shaping the diversity of natural Synechococcus and cyanophage communities over seasonal cycles and from year to year. This work provides knowledge of how individual viral-host interactions in a natural community can lead to the stable co-existence of particular species of viruses and bacteria in a coastal ecosystem over time. This project supports 15 undergraduate student researchers, a graduate student and a postdoctoral fellow who also receives training in effective practices in science teaching. Integration of the study's results into undergraduate courses and outreach activities facilitates authentic opportunities for students to contribute to research and engagement of local junior and senior high school students. The team of scientists and students are conducting a phylogenetically-informed study of natural communities of co-occurring Synechococcus and cyanophage, a model tractable system in Narragansett Bay, to characterize phage-host interactions across different scales of diversity and time. The team's goal is to isolate a large collection of ~100 Synechococcus and ~200 cyanophage from Narragansett Bay and to conduct infection assays and comparative genomics on these isolates. They employ amplicon sequencing of highly variable loci for both Synechococcus and cyanophage to characterize community dynamics across broad to fine genetic scales - ecotypes to within-species variants - for 10 years of archived monthly samples and a new weekly time-series over two years. These studies address the following three key questions: 1) Are there inherent boundaries of genetic relatedness (i.e. ecotype, species, or finer levels) at which the patterns of infection networks fundamentally shift from being mostly nested to mostly modular? (2) What are the underlying mechanisms and genetic loci that determine the boundaries of infection, i.e., host range and phage susceptibility? and, (3) How do host-phage interactions at different phylogenetic levels influence community structure over short (weeks to months) and long (year-to-year) time scales? Results from this project help to better understand how phytoplankton and bacterioplankton communities are shaped by viral predation and how host and phage diversity is created, maintained, and structured in the oceans. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.6:["$","$L1b",null,{"dataset":{"_id":"906562","description":"","problem":"Sampling was done weekly in nearly all cases, and in most cases sampling was done on Wednesdays. 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Duplicate samples for macronutrient measurements were made. This dataset reports the mean value of duplicate measurements when both measurements were above the detection limit (flag=0). In rare cases where one duplicate was measured as below detection limit and the other duplicate value was above the detection limit, the latter was reported and a flag value for that measurement is provided (flag=1).

\n","bcodmoProcessing":"$1d","title":"Water characteristics measured in Narragansett Bay over two years, from March 2021 to September 2023","nickname":"Narragansett Bay RWU Time-series","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"2","version_date":"2025-04-03T04:00:00.000Z","abstract":"$1e","citation":"Ahlgren, N. A., Marston, M. (2025) Water characteristics measured in Narragansett Bay over two years, from March 2021 to September 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2025-04-03 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.906562.2 [access date]","citationParts":{"identifier":"doi:10.26008/1912/bco-dmo.906562.2","versionDate":"2025-04-03","versionId":"2","year":"2025","title":"Water characteristics measured in Narragansett Bay over two years, from March 2021 to September 2023","authors":"Ahlgren, N. 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Date and approximate time of water sampling in the local time zone (EST/EDT) in ISO 8601 format

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Date/Time (Local) ISO formatted
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\n2009-08-30T14:05:00[.xx]Z (UTC time)
\n2009-08-30T14:05:00[.xx]-05:00
\nThe dashes and the colons can be dropped.
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Note the Time Zone (TZ) as +/-HH:MM
\nTime Zone signage is for conversion from local to UTC
\nWest Coast USA in summer is +7 (add 7 hrs to local time for UTC)

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Date and approximate time of water sampling in UTC in ISO 8601 format

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Date/Time (UTC) ISO formatted
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\nThe dashes and the colons can be dropped.
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Sample ISO_datetime_utc:
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"}},{"_id":"957860","name":"WaterTemp","format":"","frictionlessType":"number","description":"

Water temperature

","units":{"_id":"","name":"degrees Celsius"},"type":{"_id":"733","name":"temperature","description":"

water temperature at measurement depth

"}},{"_id":"957861","name":"AirTemp","format":"","frictionlessType":"integer","description":"

Air temperature in Fahrenheit

","units":{"_id":"","name":"degrees Fahrenheit"},"type":{"_id":"997","name":"temp_air","description":"

air temperature; see dataset for units of measurement, may be in degrees C or F

"}},{"_id":"957862","name":"Salinity","format":"","frictionlessType":"number","description":"

Water salinity

","units":{"_id":"","name":"Practical salinity units (PSU)"},"type":{"_id":"955","name":"sal","description":"

Salinity: The amount (mass or quantity of matter) of salt per amount (volume or mass) of a body of water.

"}},{"_id":"957863","name":"ChlA","format":"","frictionlessType":"number","description":"

Chlorophyll a

","units":{"_id":"","name":"micrograms per liter (ug/L)"},"type":{"_id":"766","name":"chlorophyll a","description":"

Total chlorophyll a pigment. See dataset for units of measurement; often reported in: milligrams per cubic meter (mg/m3); micrograms/liter (ug/L); nanograms/liter (ng/L).

CAUTION Within JGOFS MERGED water column products: this parameter is derived from HPLC methodology and the units are nanograms/liter; however; this parameter name is also used for a variety of water and nonwater column sampling; derived via the fluorometric method; units will also vary.

"}},{"_id":"957864","name":"NO2NO3","format":"","frictionlessType":"number","description":"

Water nitrate plus nitrite; BDL = below detection limit

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"902","name":"NO3_NO2","description":"

Nitrate and Nitrite, µM (micromolar) or µg-at NO3-N and NO2-N/l

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Water orthophosphate; BDL = below detection limit

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"937","name":"PO4","description":"

Orthophosphate (phosphate, reactive phosphorus), Various units.

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Water silicate; BDL = below detection limit

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1209","name":"SiO4","description":"

Silicate; reactive silica. Also referred to as dissolved silicate, dissolved silica.

"}},{"_id":"957867","name":"NH4","format":"","frictionlessType":"number","description":"

Water ammonium; BDL = below detection limit

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"901","name":"Ammonium","description":"

Ammonium and ammonia concentration parameters in any body of fresh or salt water.

"}},{"_id":"957868","name":"NO2","format":"","frictionlessType":"number","description":"

Water nitrite; BDL = below detection limit

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1192","name":"NO2","description":"

Nitrite

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Flag for ammonium data. 0 = mean of duplicate measurements; 1 = value of one of the duplicates, the other duplicate was BDL.

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Data flag. Multiple uses which are dataset dependent. Some uses: data quality, collection occurrence or success, depth indicator. See dataset documentation for specific details

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Flag for nitrate plus nitrite data. 0= mean of duplicate measurements; 1= value of one of the duplicates, the other duplicate was BDL.

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Data flag. Multiple uses which are dataset dependent. Some uses: data quality, collection occurrence or success, depth indicator. See dataset documentation for specific details

"}},{"_id":"957871","name":"Latitude","format":"","frictionlessType":"number","description":"

Approximate latitude where sample was collected

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"730","name":"latitude","description":"

latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes

"}},{"_id":"957872","name":"Longitude","format":"","frictionlessType":"number","description":"

Approximate longitude where sample was collected

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"731","name":"longitude","description":"

longitude, in decimal degrees, East is positive, negative denotes West; Reported in some datasets as degrees, minutes

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