| Contributors | Affiliation | Role |
|---|---|---|
| Winter, Christian | University of Vienna | Principal Investigator |
| Bright, Monika | University of Vienna | Co-Principal Investigator |
| Tinta, Tinkara | National Institute of Biology, Slovenia | Scientist |
| Krause, Nicole | University of Vienna | Student |
| York, Amber D. | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
Samples were collected using a suction-sampler operated onboard the remotely-operated vehicle (ROV) SuBastian. Samples for the enumeration of prokaryotic cells and viruses were immediately fixed with glutaraldehyde at a final concentration of 0.5% at room temperature for 10 minutes, flash-frozen in liquid nitrogen, and stored at -80°C until analysis by flow cytometry (Brussaard et al. 2010). Upon thawing samples were stained with SYBR Green I for 10 minutes at room temperature for prokaryotes or at 80°C for viruses. The samples were then analyzed using a FACSAria III flow cytometer (Becton Dickinson), and prokaryotes and viruses were enumerated on cytograms of side scatter versus green fluorescence (Brussaard et al. 2010).
* Sheet 1 of submitted file "insituAbundances.xls" was imported into the BCO-DMO data system for this dataset. Table will appear as Data File: 962594_v1_insitu_abundances.csv (along with other download format options).
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
* ISO DateTime with timezone (UTC) column added in ISO 8601 format.
| File |
|---|
962594_v1_insitu_abundances.csv (Comma Separated Values (.csv), 976 bytes) MD5:d2c006f4ff10831a320e091d2a2b1456 Primary data file for dataset ID 962594, version 1 |
| Parameter | Description | Units |
| subsample_id | identification number of the sample during soi cruise | unitless |
| habitat | sample location, either surface vent, subsurface vent, or deep sea (vent, non vent, subvent) | unitless |
| latitude | geographic latitude, GPS position | decimal degrees |
| longitude | geographic longitude, GPS position | decimal degrees |
| date | date of sampling | unitless |
| time | sampling time (Time zone GMT-6) | unitless |
| ISO_DateTime_UTC | Datetime with timezone of sampling (ISO 8601 format) | unitless |
| depth | depth | meters (m) |
| prokaryotes | prokaryotic cell concentration | 10000 prokaryotic cells per milliliter (10^4 mL^-1) |
| viruses | virus concentration | 10000 viruses per milliliter (10^4 mL^-1) |
| Dataset-specific Instrument Name | FACSAria III flow cytometer (Becton Dickinson) |
| Generic Instrument Name | Flow Cytometer |
| Dataset-specific Description | FACS Aria III flow cytometer: The instrument was used to enumerate prokarytes and viruses after staining with the dsDNA-specific stain SYBR Green I. |
| Generic Instrument Description | Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) |
| Dataset-specific Instrument Name | |
| Generic Instrument Name | ROV SuBastian |
| Dataset-specific Description | ROV SuBastian and its Multi-Chamber Suction Sampler: Water samples were collected with ROV SuBastian suction sampler at the vent sites. |
| Generic Instrument Description | ROV SuBastian is operated from the research vessel Falkor and the R/V Falkor(too). The ROV is outfitted with a suite of sensors and scientific equipment to support scientific data and sample collection, as well as interactive research, experimentation, and technology development. More information available at https://schmidtocean.org/technology/robotic-platforms/4500-m-remotely-op... |
| Website | |
| Platform | R/V Falkor (too) |
| Report | |
| Start Date | 2023-06-29 |
| End Date | 2023-07-28 |
| Description | R/V Falkor (too) cruise to the East Pacific Rise (9°N 104°W) as part of the project "The Underworld of Hydrothermal Vents" which used the sub ROV SuBastian |
Since the discovery of deep-sea hydrothermal vents in 1977, scientists realize that life exists above and below the seafloor. The extent to which they are interconnected, however, remains poorly understood. We propose to characterize subsurface biosphere diversity, from viruses to animals, at deep-sea hydrothermal vents to elucidate the nature and extent of connectivity between the surficial and subsurface biospheres. We will test the hypotheses that 1) eukaryotic life is also an integral component of the subseafloor biosphere; 2) the subseafloor is inhabited by both cosmopolitan and endemic protists and fungi; and 3) the subseafloor habitats harbor larvae -and perhaps adult life stages- of vent endemic animals. This research will transform our understanding of the ecology/evolution of subseafloor habitats and our thinking about animal recruitment at deep-sea vents. Consistent with SOI's mission, we will expand our knowledge of limits of eukaryotic life. During a three-week cruise with R/V Falkor and ROV SuBastian to "East Pacific Rise" vents at the 9º50'N region, we will use in situ and lab experiments, molecular identification with amplicon sequencing, Illumina NextSeq 500 technology, 2bRAD population genetic analyses, and RT qPCR for functional analyses. Our data management plan includes deposition of metadata/samples to long-term repositories. Outreach includes inquiry-learning telepresence programs for children from USA and Europe.
| Funding Source | Award |
|---|---|
| Schmidt Ocean Institute (SOI) |