14:I[71133,["4507","static/chunks/e2799680-446c489506865058.js","7665","static/chunks/7665-81c5407bc4489ff9.js","8500","static/chunks/8500-e4dd1cb1bb626827.js","9753","static/chunks/9753-028c0a26aa71f2d4.js","8437","static/chunks/8437-a09d0b83fcec76af.js","4557","static/chunks/4557-64c5efa221bc528f.js","947","static/chunks/947-410627998426400f.js","5357","static/chunks/5357-6142894e88cb7da2.js","8382","static/chunks/app/dataset/%5Bid%5D/data/view/page-177ea8adea7aed86.js"],"default"] 15:T54c,

Collection and analysis of "live" (unprocessed) water samples and separate preserved water samples for assessment of potential toxin-producing phytoplankton was done monthly. Samples were processed by Dr. Eli Meyer of Aquabiomics Inc. (Junction City, OR), by Dr. Ralph Elston of Aquatechnics (Carlsborg, WA), by Dr. Maria Kavanaugh of OSU's College of Earth, Ocean, and Atmospheric Sciences (Seascape Ecology Lab), and Dr. Manuel Garcia-Jaramillo in the Department of Environmental and Molecular Toxicology at OSU.

IFCB sampling following Dr. Kavanaugh's protocol: replicate (n = 3) bottle samples were collected at each site in September and November 2023, and in January, February, and June 2024. Dataset rows are from replicate bottle samples. Live samples are allowed to settle, then processed using Image FlowCytoBot (IFCB) sampling for the presence and abundance of preservation-sensitive phytoplankton (e.g., "naked" flagellates). Hardier plankters can be examined in both live and preserved samples.

Dataset rows: Each row corresponds to an instrument run. Generally, that would be one per sample bottle, but on occasion, samples would be run in duplicate (e.g., if the instrument had an issue with the first run), in which case a single sample might be accounted for in more than one row. The date is embedded in the File ID.

16:T6ee,This section documents curation actions performed prior to publication review with the submitter, and additional information relevant to understanding and reusing this dataset. It distinguishes changes made to the submitted (meta)data from unresolved issues and/or enhancements that improve future reuse and interoperability. CURATION ACTIONS PERFORMED ON DATA - Imported site metadata from "Copy of 6590_menge_site_descrip.xlsx" (sheet 1) into the BCO-DMO system. - Imported IFCB biovolume data from "IFCB analysis of biovolumes of key taxa_10_17_24.xlsx" (sheet 1) into the BCO-DMO system. - Renamed fields to comply with BCO-DMO naming conventions. - Joined the site list to the IFCB biovolume data matching on site code, adding fields: "site_lat", "site_lon", and "site_name". - Converted "date" field from format "%m-%d-%y" to ISO date format "%Y-%m-%d". - Converted "Date_Run_on_IFCB" from format "%m/%d/%Y" to ISO date format "%Y-%m-%d". - Saved the final data file as "994284_v1_ifcb_biovolumes_key_taxa.csv". CURATION ACTIONS PERFORMED ON METADATA - BCO-DMO's standard metadata entry and text formatting steps were performed. See: https://www.bco-dmo.org/how-to/standard-curation-edits ISSUES POTENTIALLY IMPACTING REUSE - This dataset has been published "as is". Final review by the data submitter was not received after the data were imported into the BCO-DMO data system. - The following column descriptions were not provided or are ambiguous: -- "date" was assumed to be date of sample collected; not confirmed by data provider. -- "File_Comment" was not described. -- "File_ID" was assumed to be the IFCB file name. -- In the "Preserved_Live column", descriptions were not provided for "LIVE (DECANTED)" and "LIVE (CONCENTRATED)".17:Tabe,NSF Award Abstract: On temperate rocky shores, mussels are a 'foundation' species, serving as prey for multiple predators, able to filter particles out of huge volumes of water, and harboring hundreds of other species, thereby serving a central role in community function. Hence, threats to their abundance and persistence are of significant ecological and societal concern. Past research has shown that mussels are highly resilient and well-adapted to acute, short-lived stresses such as disturbance from winter storms or short-term warming. In summer 2023, a novel pattern of mortality was discovered during routine field research: high numbers of dead mussels, many with tissue remaining in the shell were observed in a scattered pattern. That is, rather than a mass mortality (i.e., all mussels in a large area are killed), single dead mussels surrounded by live and apparently healthy mussels occurred, with multiple dead individuals per square meter. This project investigates the cause of the mortality event, which preliminary data suggests may be a harmful algal bloom. The project also builds capacity by supporting research training for several undergraduate students and informs the public and resource managers about an emerging threat to the stability of rocky intertidal ecosystems. The hypothesis under investigation is that the 2023 'scattered' mortality event at 8 study sites along the Oregon and northern California coasts affecting the rocky intertidal dominant, Mytilus californianus, may be an unusual consequence of a harmful algal bloom. During this event, densities of dead mussels have ranged from 0.7 to 10.6/m2. This novel pattern contrasts with typical mass mortalities of organisms where all individuals within a specific site or area die due to (e.g.) thermal stress or severe storms. The scattered mortality pattern persisted through late September 2023. Preliminary histological evidence suggests the ability of mussels to digest food is being suppressed, most likely by a water-borne toxin. To investigate the cause of the mortality event, the team is (1) collecting monthly (a) water samples for identification of dinoflagellate species and (b) mussel tissue samples for histological investigation of digestive and kidney tissues and high performance liquid chromatography and mass spectrometry analyses for the presence of algal-derived toxins; (2) continuing mussel bed surveys to determine the seasonality of the mortality event; and (3) quantifying mussel bed persistence through winter storms at sites with higher or lower rates of mortality. This research advances understanding of community responses to intense environmental stresses and how these might influence the future of rocky intertidal ecosystems.6:["$","$L14",null,{"dataset":{"_id":"994284","description":"","problem":"See \"BCO-DMO Curation Notes\".","acquisition":"$15","processing":"","bcodmoProcessing":"$16","title":"Results of imaging flow cytobot (IFCB) analysis of biovolumes of key phytoplankton taxa from waters collected near study sites along the rocky intertidal on the Oregon coast in 2023 and 2024","nickname":"IFCB analysis of biovolumes of key phytoplankton taxa","dataUrl":"","currentState":"Final","currentStateId":"7","validated":false,"restricted":false,"version":"1","version_date":"2026-04-02T04:00:00.000Z","abstract":"This data set contains results of imaging flow cytobot (IFCB) analysis of biovolumes of key phytoplankton taxa from waters collected near study sites along the rocky intertidal on the Oregon coast. Study sites include: Fogarty Creek, Boiler Bay, Yachats Beach, Strawberry Hill, Tokatee Klootchman, Cape Blanco, Rocky Point, and Kibesillah Hill. Data were generated via field sampling and lab processing from 2023 to 2024. These data were collected to quantify the mortality pattern and attempt to determine the cause.","citation":"Menge, B. A., Rothman, B., Vinton, A. (2026) Results of imaging flow cytobot (IFCB) analysis of biovolumes of key phytoplankton taxa from waters collected near study sites along the rocky intertidal on the Oregon coast in 2023 and 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-04-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.994284.1 [access date]","citationParts":{"identifier":"doi:10.26008/1912/bco-dmo.994284.1","versionDate":"2026-04-02","versionId":"1","year":"2026","title":"Results of imaging flow cytobot (IFCB) analysis of biovolumes of key phytoplankton taxa from waters collected near study sites along the rocky intertidal on the Oregon coast in 2023 and 2024","authors":"Menge, B. A., Rothman, B., Vinton, A.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.26008/1912/bco-dmo.994284.1","release_date":null,"field_dataset_location":"Rocky intertidal on the Oregon coast","temporal_extent":{"start":"2023-09-13T04:00:00.000Z","end":"2024-06-06T04:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1694577600","label":"2023-09-13"},"end":{"value":"1717646400","label":"2024-06-06"}},"spatial_extent":{"shape":"polygon","center":{"latitude":44.548449999319,"longitude":-124.09589999814},"wkt":"POLYGON ((-124.12 44.2525, -124.0718 44.2525, -124.0718 44.8444, -124.12 44.8444, -124.12 44.2525))","north":44.8444,"east":-124.0718,"south":44.2525,"west":-124.12},"dataset_type":["Other Field Results"],"keywords":["oceans"],"data_files":[{"id":"1010852","title":"994284_v1_ifcb_biovolumes_key_taxa.csv","url":"https://datadocs.bco-dmo.org/dataset/994284/file/4WA25qPsRyJNwj/994284_v1_ifcb_biovolumes_key_taxa.csv","showTableUrl":"https://www.bco-dmo.org/dataset/994284/data/view/1010852","description":"Primary data file for dataset ID 994284, version 1","filename":"994284_v1_ifcb_biovolumes_key_taxa.csv","bytesize":14725,"checksum":{"value":"978e4b97ff8c054b1cfc158bcf221d12","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"bcodmo_dataset_994284_v1","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"51222","name":"Bruce A. Menge"},"performedAtOrganization":{"_id":"9","name":"Oregon State University"}},{"role":{"_id":"130","name":"Scientist"},"performedBy":{"_id":"994288","name":"Brenna Rothman"},"performedAtOrganization":{"_id":"9","name":"Oregon State University"}},{"role":{"_id":"130","name":"Scientist"},"performedBy":{"_id":"994291","name":"Audrey Vinton"},"performedAtOrganization":{"_id":"27","name":"University of Washington"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"51498","name":"Shannon Rauch"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[],"funding":[{"_id":"910809","name":"OCE-2346837","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=2346837"}],"instruments":[{"_id":"994296","name":"Imaging FlowCytoBot, McLane Scientific","description":"Gain settings: PMT A: 0.65, PMT B: 0.55. PMT thresholds: PMTA: 0.15, PMTB: 0.14. Flow rate of 5 ml/20 min per sample. Subset of samples were live seawater samples with no preservative, most were fixed in parformaldehyde (0.4% final concentration).","type_id":"840093","type_name":"Imaging FlowCytobot","type_desc":"The Imaging FlowCytobot (IFCB) is an in-situ automated submersible imaging flow cytometer that generates images of particles in-flow taken from the aquatic environment. https://mclanelabs.com/imaging-flowcytobot/","type_acronym":"IFCB"}],"parameters":[{"_id":"995868","name":"Preserved_Live","format":"","frictionlessType":"string","description":"

Indicates live or preserved samples

","units":{"_id":"","name":"unitless"},"type":{"_id":"1741","name":"sample_descrip","description":"

text description or other traits of the sample collected

"}},{"_id":"995869","name":"site","format":"","frictionlessType":"string","description":"

Sampling site code

","units":{"_id":"","name":"unitless"},"type":{"_id":"1124","name":"site","description":"

Sampling site identification.

"}},{"_id":"995870","name":"site_name","format":"","frictionlessType":"string","description":"

Sampling site name

","units":{"_id":"","name":"unitless"},"type":{"_id":"1124","name":"site","description":"

Sampling site identification.

"}},{"_id":"995871","name":"site_lat","format":"","frictionlessType":"number","description":"

Sampling site latitude

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"730","name":"latitude","description":"

latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes

"}},{"_id":"995872","name":"site_lon","format":"","frictionlessType":"number","description":"

Sampling site longitude

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"731","name":"longitude","description":"

longitude, in decimal degrees, East is positive, negative denotes West; Reported in some datasets as degrees, minutes

"}},{"_id":"995873","name":"date","format":"%Y-%m-%d","frictionlessType":"date","description":"

Date of sample collection

","units":{"_id":"","name":"unitless"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"995874","name":"File_Comment","format":"","frictionlessType":"string","description":"

[not defined]

","units":{"_id":"","name":"unitless"},"type":{"_id":"778","name":"comment","description":"

free text comments, may only have meaning to submitting PI

"}},{"_id":"995875","name":"Date_Run_on_IFCB","format":"%Y-%m-%d","frictionlessType":"date","description":"

Date run on IFCB

","units":{"_id":"","name":"unitless"},"type":{"_id":"1070","name":"date","description":"

"}},{"_id":"995876","name":"File_ID","format":"","frictionlessType":"string","description":"

IFCB file name

","units":{"_id":"","name":"unitless"},"type":{"_id":"659543","name":"file_name","description":"

File name

"}},{"_id":"995877","name":"Volume_Processed_by_Instrument_ml","format":"","frictionlessType":"number","description":"

Volume processed by the IFCB

","units":{"_id":"","name":"milliliters (mL)"},"type":{"_id":"1871","name":"sample_volume","description":"

volume of the sample, i.e., sample size

"}},{"_id":"995878","name":"Diatom_microns3_ml","format":"","frictionlessType":"number","description":"

Diatom biovolume

","units":{"_id":"","name":"cubic microns per milliliter"},"type":{"_id":"754","name":"biovolume","description":"