Collection and analysis of "live" (unprocessed) water samples and separate preserved water samples for assessment of potential toxin-producing phytoplankton was done monthly. Samples were processed by Dr. Eli Meyer of Aquabiomics Inc. (Junction City, OR), by Dr. Ralph Elston of Aquatechnics (Carlsborg, WA), by Dr. Maria Kavanaugh of OSU's College of Earth, Ocean, and Atmospheric Sciences (Seascape Ecology Lab), and Dr. Manuel Garcia-Jaramillo in the Department of Environmental and Molecular Toxicology at OSU.
IFCB sampling following Dr. Kavanaugh's protocol: replicate (n = 3) bottle samples were collected at each site in September and November 2023, and in January, February, and June 2024. Dataset rows are from replicate bottle samples. Live samples are allowed to settle, then processed using Image FlowCytoBot (IFCB) sampling for the presence and abundance of preservation-sensitive phytoplankton (e.g., "naked" flagellates). Hardier plankters can be examined in both live and preserved samples.
Dataset rows: Each row corresponds to an instrument run. Generally, that would be one per sample bottle, but on occasion, samples would be run in duplicate (e.g., if the instrument had an issue with the first run), in which case a single sample might be accounted for in more than one row. The date is embedded in the File ID.