Dataset: Ocean warming & acidification experiment: Acartia tonsa survival

Name: Acartia tonsa survival data for transgenerational ocean warming and acidification data
Published: 2023-09-07
Keywords: copepod, adaptation, climate change, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.906222.1

Project:

Collaborative Research: Response of marine copepods to warming temperature and ocean acidification (Copepod Response to Warming Temp and OA)

Principal Investigator:

Hans G. Dam (University of Connecticut, UConn)

Co-Principal Investigator:

Hannes Baumann (University of Connecticut, UConn)

Michael Finiguerra (University of Connecticut, UConn)

Melissa Pespeni (University of Vermont, UVM)

Scientist:

Reid Brennan (University of Vermont, UVM)

Student:

James deMayo (University of Connecticut, UConn)

Gihong Park (University of Connecticut, UConn)

Contact:

James deMayo (University of Connecticut, UConn)

Technician:

Lydia Norton (University of Connecticut, UConn)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-09-07

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Acartia tonsa survival data for transgenerational ocean warming and acidification data

Abstract:

These data include survivorship measurements collected for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments. Data was collected as the proportion of surviving individuals on any day (x) relative to the starting number of individuals for an single experiment.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2023-09-07	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.906222.1	2023-09-07

Description

These data are part of at multigenerational experiment of Arcatia tonsa exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments.

Methods & Sampling

Survival was measured from nauplius 1 (N1) stage to copepodid 6 (adult) stage. For a given generation, all adults from the previous generation were removed from the culture and allowed to lay eggs in food-replete media for 48 h. Resulting nauplii were chosen for tracking survival. Unhatched eggs and any nauplii not chosen for survival analysis were returned to their respective replicate cultures for continued population maintenance. To measure survival for all generations where life-history traits were evaluated, three 250-mL beakers for each replicate culture were supplied with 25 randomly chosen N1 nauplii each and housed in the plexiglass enclosure described above (n = 21 per treatment). Copepods were checked every 48-72 h. The number of dead, live, and missing copepods were logged for each beaker along with general stage (i.e. nauplius, copepodite, adult female, or adult male). Nauplii were grown with media at levels of 500 μg C L-1 to prevent overgrowth of phytoplankton and allow for adequate nauplii grazing. Following the naupliar stages, copepods were grown with food-replete (800 μg C L-1) media as described earlier. Food was replaced with fresh media on monitoring days. The target (actual ± standard deviation) conditions were as follows: ambient (AM) temperature = 18 °C (18 ± 0.34, N = 330), AM pCO2= 400 μatm (379 ± 36, N = 18; pH = 8.26 ± 0.1, N = 330); high temperature = 22 °C (22 ± 0.81, N = 336); and high pCO2= 2,000 µatm (2,301 ± 215, N = 18; pH = 7.55 ± 0.08, N = 330). AM target levels represented extant conditions for this species in northeast Atlantic estuaries.

Data Processing Description

The fraction of survived individuals (lx) was calculated as nx/ni where nx represents the number of live individuals on day x, and ni represents initial individuals. Average survival was calculated per each replicate culture at each generation measured. Differences in day-specific survival between replicates and treatments was assessed using the ‘survival’ package in R.

Data Files

File
906222_v1_survival.csv (Comma Separated Values (.csv), 117.58 KB) MD5:ee08914513f0ec4ffff3cf3d027bb4e1 Primary data file for dataset 906222

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1559180
NSF Division of Ocean Sciences	OCE-1559075

Deployments

None available yet

Instruments

Microscope - Optical

Supplied Name: Olympus SZH-ILLD stereoscope

Supplied Description:

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Generation	The corresponding generation when survival was measured.	unitless	sample_descrip
Treatment	The corresponding treatment evaluated.	unitless	treatment
Temp	The target temperature in Celsius for each treatment.	degrees Celsius(°C)	temperature
pH	The pH for each treatment	unitless	pH
Rep	The biological culture replicate for each treatment.	unitless	replicate
Beak	The replicate 25mL beaker used in survival assays.	unitless	sample_descrip
time	The number of days after the start of the experiment.	unitless	time_elapsed
nx	The number of surviving individuals.	unitless	count
lx	The proportion of surviving individuals remaining.	unitless	ratio

Database

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Dataset: Ocean warming & acidification experiment: Acartia tonsa survival