Dataset: Ocean warming & acidification experiment: Acartia tonsa development time

Name: Acartia tonsa development time for transgenerational experiment
Published: 2023-09-07
Keywords: copepod, adaptation, climate change, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.906188.1

Project:

Collaborative Research: Response of marine copepods to warming temperature and ocean acidification (Copepod Response to Warming Temp and OA)

Principal Investigator:

Hans G. Dam (University of Connecticut, UConn)

Co-Principal Investigator:

Hannes Baumann (University of Connecticut, UConn)

Michael Finiguerra (University of Connecticut, UConn)

Melissa Pespeni (University of Connecticut, UConn)

Scientist:

Reid Brennan (University of Vermont, UVM)

Student:

James deMayo (University of Connecticut, UConn)

Gihong Park (University of Connecticut, UConn)

Contact:

James deMayo (University of Connecticut, UConn)

Technician:

Lydia Norton (University of Connecticut, UConn)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-09-07

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Acartia tonsa development time for transgenerational experiment

Abstract:

These data include development (i.e. maturation) time measurements for A. tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments. Data were calculated for naupliar development time (i.e. naupliar stage 1 to naupliar stage 6) and copepodite development time (i.e. copepodite stage 1 to adulthood).

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2023-09-07	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.906188.1	2023-09-07

Description

These data are part of at multigenerational experiment of Arcatia tonsa exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments.

Methods & Sampling

Development time was measured on 75 individuals from each replicate culture per treatment (n=4 per treatment). The 75 individuals were split among three 25-mL beakers. Life stage was measured every 2-3 days and recorded as either naupliar or copepodite. Average time through naupliar and copepodite development was calculated based on the proportion of individuals occupying each life stage over time. The target (actual ± standard deviation) conditions were as follows: ambient (AM) temperature = 18 °C (18 ± 0.34, N = 330), AM pCO2= 400 μatm (379 ± 36, N = 18; pH = 8.26 ± 0.1, N = 330); high temperature = 22 °C (22 ± 0.81, N = 336); and high pCO2= 2,000 µatm (2,301 ± 215, N = 18; pH = 7.55 ± 0.08, N = 330). AM target levels represented extant conditions for this species in northeast Atlantic estuaries.

Data Processing Description

Data was processed with R v4.0. by calculating a weighted average of the time it took to mature from nauplii to copepodite or from copepodite to adult.

Data Files

File
906188_v1_devtime.csv (Comma Separated Values (.csv), 126.30 KB) MD5:f56845bc3c537b897e24a43d96a27f01 Primary data file for dataset 906188.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1559180
NSF Division of Ocean Sciences	OCE-1559075

Deployments

None available yet

Instruments

Microscope - Optical

Supplied Name: Olympus SZH-ILLD stereo microscope

Supplied Description:

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Generation	the generation where the development time was measured.	unitless	stage
Treatment	the treatment (1=AM, 2=OA, 3=OW, 4=OWA).	unitless	treatment
Temp	the temperature condition of the relevant treatment	degrees Celsius (°C)	temp
pH	The pH condition of the relevant treatment	unitless	pH
Rep	The biological culture replicate	unitless	replicate
Beak	The 25-mL beaker within a culture replicate	unitless	sample_descrip
time	The day at which the beaker was measured	unitless	time
nx	The number of individuals at a particular time	unitless	count
Ndev	The number of individuals that matured from nauplii to copepodites on any given day.	unitless	count
Cdev	The number of individuals that matured from copepodites to adulthood on any given day.	unitless	count

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Database

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Dataset: Ocean warming & acidification experiment: Acartia tonsa development time