Dataset: qPCR assays
Data Citation:
Buzzoni, D., Cunning, R., Baker, A. (2024) Results from qPCR assays to quantify the abundance and photochemical performance of symbionts relative to coral cells in three coral species collected from colonies in southeast Florida in April and October 2019 before, during, and after heat stress tests. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-01-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.918220.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
DOI:10.26008/1912/bco-dmo.918220.1
Spatial Extent: N:25.6788 E:-80.0974 S:25.6773 W:-80.1005
Emerald Reef off Key Biscayne, SE Florida (USA)
Temporal Extent: 2019-05-22 - 2020-03-05
Principal Investigator:
Andrew Baker (University of Miami Rosenstiel School of Marine and Atmospheric Science, UM-RSMAS)
Co-Principal Investigator:
Ross Cunning (Shedd Aquarium)
Student:
Daisy Buzzoni (University of Miami Rosenstiel School of Marine and Atmospheric Science, UM-RSMAS)
BCO-DMO Data Manager:
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2024-01-22
Restricted:
No
Validated:
Yes
Current State:
Final no updates expected
Results from qPCR assays to quantify the abundance and photochemical performance of symbionts relative to coral cells in three coral species collected from colonies in southeast Florida in April and October 2019 before, during, and after heat stress tests
Abstract:
This dataset contains results from assays to quantify the abundance and photochemical performance of Breviolum, Cladocopium, and Durusdinium symbionts relative to coral cells in Montastraea cavernosa, Orbicella faveolata, and Siderastrea siderea corals collected from colonies in southeast Florida in April 2019 and in October 2019, before, during and after aquarium-based experimental heat stress tests. Bulk genomic DNA was extracted from tissue scrapings taken from 2.5cm diameter cores of corals and was used as a template for symbiont genus-specific qPCR assays. The resulting CT values were used to calculate the relative abundance of each symbiont genus within each coral core over the course of the heat stress. The photochemical efficiency of each coral core was also measured periodically throughout heat stress tests using an imaging pulse amplitude modulated (I-PAM) fluorometer. The publication based on these data is Buzzoni, et al. (2023) (DOI: 10.1007/s00338-023-02428-x).