Dataset: Trichodesmium physiology and cell growth
Deployment: lab_Webb_Hutchins_Fu

Trichodesmium growth rate and cell size related to pCO2 and nutrients

Principal Investigator:

David A. Hutchins (University of Southern California, USC)

Co-Principal Investigator:

Eric A. Webb (University of Southern California, USC)

Contact:

David A. Hutchins (University of Southern California, USC)

BCO-DMO Data Manager:

Ms Dicky Allison (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Adaptation of key N2-fixing cyanobacteria to changing CO2 (HiCO2_AdaptCyano)

Current State:

Final no updates expected

Version:

Final

Data URL:

https://www.bco-dmo.org/dataset-deployment/649911/data

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Description

Trichodesmium erythraeum growth rates per day for all replicates in each treatment calculated from cell counts. Trichodesmium erythraeum was grown in biological triplicate under two CO2 regimes (380 and 750 uatm pCO2) and four different nutrient regimes (replete, phosphorus-limited,iron-limited, iron/phosphorus colimited).

These data were utilized in the following publications:

Walworth, N. G., Fu, F. X., Webb, E. A., Saito, M. A., Moran, D., Mcllvin, M. R. & Hutchins, D. A. (2016a). Mechanisms of increased Trichodesmium fitness under iron and phosphorus co-limitation in the present and future ocean. Nature Communications, 7, 12081. doi: 10.1038/ncomms12081

Walworth, N. G., Lee, M. D., Suffridge, C., Qu, P., Fu, F., Saito, M. A., ... & Hutchins, D. A. (2018). Functional genomics and phylogenetic evidence suggest genus-wide cobalamin production by the globally distributed marine nitrogen fixer Trichodesmium. Frontiers in Microbiology, 9, 189. doi: 10.3389/fmicb.2018.00189

Genetic sequences from the Trichodesmium erythraeum used in this experiment can be accessed at The National Center for Biotechnology Information (NCBI) under the BioProject PRJNA312342.

Walworth, N. G., Lee, M. D., Fu, F. X., Hutchins, D. A., ... & Webb, E. A.(2016b). Molecular and physiological evidence of genetic assimilation to high CO2 in the marine nitrogen fixer Trichodesmium. Proceedings of the National Academy of Sciences, 113(47), E7367-E7374. doi: 10.1073/pnas.1605202113

Methods & Sampling

Dataset acquisition description

The following are excerpts from the Nature Communication paper Walworth et al. 2016a. Please refer to this reference for more details of the methodology used to generate these data.

"We grew the nitrogen-fixing cyanobacterium Trichodesmium for 1 year under Fe/P co-limitation following 7 years of both low and high CO2 selection. "

"Growth rates were calculated according to microscopic cell counts for reported values, or using in vivo chlorophyll fluorescence measurements with a Turner 10 AU fluorometer for semi-daily dilution calculations in real time during the experiments. Comparisons between cell counts and in vivo-based growth rates revealed no significant differences between the two methods of assessing biomass changes."

A picogram carbon/µm filament was used for all replicates in each treatment (cell size proxy).

Data Processing Description

Dataset Processing Description

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1260233	NSF Division of Ocean Sciences

Instruments

Turner Designs Fluorometer 10-AU

Supplied Name: Turner 10 AU fluorometer

Supplied Description:

Instrument Type

Generic Name: Turner Designs Fluorometer 10-AU

Acronym: Turner Fluorometer 10-AU

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/

Generic Description:

The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Treatments	CO2 and Nutrient regimes replicated three times; +P and -P speak of non-limiting or limiting phosphorus (20 μM and 0.5 μM phosphate respectively); +Fe or -Fe speak of limiting or non-limiting iron (500 nM iron); 380 or 750 indicate the partial pressure of CO2	micromoles;nanomoles;uatm	treatment
pg_C_per_um_filament	Picogram carbon per micron filament for all replicates in each treatment (cell size proxy)	picogram	cell_size
Growth_rates	rates calculated from cell counts	reciprocal time - per day (d-1) - because the cell numbers cancel out	growth

Database

Contribute Data

Dataset: Trichodesmium physiology and cell growth
Deployment: lab_Webb_Hutchins_Fu

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Trichodesmium physiology and cell growthDeployment: lab_Webb_Hutchins_Fu

Dataset acquisition description

Dataset Processing Description

Dataset: Trichodesmium physiology and cell growth
Deployment: lab_Webb_Hutchins_Fu