Dataset: Aligned mtDNA haplotypes
Deployment: Belize_2010

Aligned mtDNA haplotype data from the Belizean Barrier Reef in 2012.

Principal Investigator:

Peter Buston (Boston University, BU)

Co-Principal Investigator:

Cassidy C. D'Aloia (Woods Hole Oceanographic Institution, WHOI)

Contact:

Peter Buston (Boston University, BU)

BCO-DMO Data Manager:

Hannah Ake (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

An Integrative Investigation of Population Connectivity Using a Coral Reef Fish (Elacatinus Dispersal I)

Current State:

Final no updates expected

Version:

Data URL:

https://www.bco-dmo.org/dataset-deployment/738701/data

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Description

Aligned mtDNA haplotype data from the Belizean Barrier Reef in 2012.

Methods & Sampling

Dataset acquisition description

We collected tissue samples from 300 Elacatinus lori individuals at ten locations across the Belize barrier reef for population genetic analyses. We marked the location of each collection site with a Garmin GPSMAP 76Cx unit. Individuals were caught with slurp guns and we took a small clip from the caudal fin using scissors. All tissue samples were store in 95% EtOH. Genetic data were collected based on standard DNA extractions (DNEasy kits, Qiagen), and PCRs. Further details on all methods can be found in D’Aloia et al. (2014), Molecular Ecology.

Data Processing Description

Dataset Processing Description

Mitochondrial cytb sequences were processed in CODONCODE ALIGNER V.4.0.4. We joined forward and reverse contigs, then aligned them using the ClustalW algorithm. We retained 960 bp of high-quality sequence.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1260424	NSF Division of Ocean Sciences

Instruments

GPS receiver

Supplied Name: Garmin GPSMAP 76Cx unit

Supplied Description:

Used to mark site locations

Instrument Type

Generic Name: GPS receiver

Generic Description:

Acquires satellite signals and tracks your location.

This term has been deprecated. Use instead: https://www.bco-dmo.org/instrument/560

Parameters

None available yet

Database

Contribute Data

Dataset: Aligned mtDNA haplotypes
Deployment: Belize_2010

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Aligned mtDNA haplotypesDeployment: Belize_2010

Dataset acquisition description

Dataset Processing Description

Dataset: Aligned mtDNA haplotypes
Deployment: Belize_2010