Dataset: Aligned mtDNA haplotypes

Name: Aligned mtDNA haplotype data from the Belizean Barrier Reef in 2012.
Published: 2018-06-18
Keywords: oceans

Cite This Dataset

Spatial Extent: N:16.789722 E:-88.075833 S:16.789722 W:-88.075833

Temporal Extent: 2012 - 2012

Project:

An Integrative Investigation of Population Connectivity Using a Coral Reef Fish (Elacatinus Dispersal I)

Principal Investigator:

Peter Buston (Boston University, BU)

Co-Principal Investigator:

Cassidy C. D'Aloia (Woods Hole Oceanographic Institution, WHOI)

Contact:

Peter Buston (Boston University, BU)

BCO-DMO Data Manager:

Hannah Ake (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-06-18

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Data URL:

https://www.bco-dmo.org/dataset/738694/data

Aligned mtDNA haplotype data from the Belizean Barrier Reef in 2012.

Expand/Collapse All

Description

Aligned mtDNA haplotype data from the Belizean Barrier Reef in 2012.

Methods & Sampling

We collected tissue samples from 300 Elacatinus lori individuals at ten locations across the Belize barrier reef for population genetic analyses. We marked the location of each collection site with a Garmin GPSMAP 76Cx unit. Individuals were caught with slurp guns and we took a small clip from the caudal fin using scissors. All tissue samples were store in 95% EtOH. Genetic data were collected based on standard DNA extractions (DNEasy kits, Qiagen), and PCRs. Further details on all methods can be found in D’Aloia et al. (2014), Molecular Ecology.

Data Processing Description

Mitochondrial cytb sequences were processed in CODONCODE ALIGNER V.4.0.4. We joined forward and reverse contigs, then aligned them using the ClustalW algorithm. We retained 960 bp of high-quality sequence.